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The map label for this gene is ipk

Identifier: 28377360

GI number: 28377360

Start: 412982

End: 413848

Strand: Direct

Name: ipk

Synonym: lp_0460

Alternate gene names: 28377360

Gene position: 412982-413848 (Clockwise)

Preceding gene: 28377359

Following gene: 28377361

Centisome position: 12.48

GC content: 49.48

Gene sequence:

>867_bases
GTGCAGATCGTTGAAAAAGCACCGGCCAAAATCAATTTAGGACTGGATACCTTATTTGAACATCCAAATGGGGATAAAGA
GTGGGACATGGTGATGACGTCGGTCGATCTGGCCGACTATGTCATGTTAGAATCATTGCATACGAACCGAATCGAAGTGG
TCACCGATAGTGGCTTTTTGCCGAATGATCGCCGTAACTTAGCTTTTCAAGCGGTGAGCGTTTTAAAACGGTATTGTCAT
GTTGATCGGGGTGTCCGTATCAAAATAAGAAAGGCCATTCCGGTAGCGGCTGGTCTAGGAGGCGGCTCATCTGATGCCGC
CGCTGTTTTACGGGGCTTGAACCGGATGTGGAATCTCCACTTGGACTTAGCCACGTTAGCACGTTTGGGATTGCAAGTAG
ATTCGGATGTGCCATACTGTGTCTACAGTCAGACGGCCCACGTGACGGGAAAAGGCGATGTGGTTACCCCACTGCCTAAG
TTGCCGCCGATGTGGATTATTCTAGCGAAGCCGAAAGTCAGTGTGTCAACGCCAAATATTTTGCGGCAGGTCAATTACGA
GCGCATCGACCAGCATCCCAATATTGAGGCCTTGTTGGCTGGTATTCAACAGCAGGATTTCGCGGAGATTTTTGCGAATA
TGGGCAACGTTTTAGAGCCAATCACGGCAAAACGCTACCCCGAGATTTTACAGATCAAGCGGCAATTGTTAACGTTTGGG
GCGGATGCGGCCCAGATGAGCGGCACCGGACCAACTGTGTTCGGTGTGTGTCGCAAACAATCGCGGGCGCAACGCGTGTA
TAATTCCTTGAAGGGTTTTTGTCGGGAAGTATACCTTGTGCGGCCGGTTAATTTAAATGAACATTAG

Upstream 100 bases:

>100_bases
CCCAAGCAAGGTCTTTTTATTTTGCCTTGATATTAGTATAATTGTATCAAAGTAAGTCAGCGACCCCGGGTAATCGCGGT
CATGGAAGAGGGTGAGACGC

Downstream 100 bases:

>100_bases
ACCGCAGTAGTCCGCCGTGATGGTGGGCTTTTTTTATTGGTACGGGCTTAGGTGAGCCGAGTCTCTAATTTACTGCTACG
CTTAAACGGTAGCGCTGAGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MQIVEKAPAKINLGLDTLFEHPNGDKEWDMVMTSVDLADYVMLESLHTNRIEVVTDSGFLPNDRRNLAFQAVSVLKRYCH
VDRGVRIKIRKAIPVAAGLGGGSSDAAAVLRGLNRMWNLHLDLATLARLGLQVDSDVPYCVYSQTAHVTGKGDVVTPLPK
LPPMWIILAKPKVSVSTPNILRQVNYERIDQHPNIEALLAGIQQQDFAEIFANMGNVLEPITAKRYPEILQIKRQLLTFG
ADAAQMSGTGPTVFGVCRKQSRAQRVYNSLKGFCREVYLVRPVNLNEH

Sequences:

>Translated_288_residues
MQIVEKAPAKINLGLDTLFEHPNGDKEWDMVMTSVDLADYVMLESLHTNRIEVVTDSGFLPNDRRNLAFQAVSVLKRYCH
VDRGVRIKIRKAIPVAAGLGGGSSDAAAVLRGLNRMWNLHLDLATLARLGLQVDSDVPYCVYSQTAHVTGKGDVVTPLPK
LPPMWIILAKPKVSVSTPNILRQVNYERIDQHPNIEALLAGIQQQDFAEIFANMGNVLEPITAKRYPEILQIKRQLLTFG
ADAAQMSGTGPTVFGVCRKQSRAQRVYNSLKGFCREVYLVRPVNLNEH
>Mature_288_residues
MQIVEKAPAKINLGLDTLFEHPNGDKEWDMVMTSVDLADYVMLESLHTNRIEVVTDSGFLPNDRRNLAFQAVSVLKRYCH
VDRGVRIKIRKAIPVAAGLGGGSSDAAAVLRGLNRMWNLHLDLATLARLGLQVDSDVPYCVYSQTAHVTGKGDVVTPLPK
LPPMWIILAKPKVSVSTPNILRQVNYERIDQHPNIEALLAGIQQQDFAEIFANMGNVLEPITAKRYPEILQIKRQLLTFG
ADAAQMSGTGPTVFGVCRKQSRAQRVYNSLKGFCREVYLVRPVNLNEH

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=29.3286219081272, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_LACPL (Q88Z91)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784252.1
- ProteinModelPortal:   Q88Z91
- SMR:   Q88Z91
- GeneID:   1064146
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0460
- NMPDR:   fig|220668.1.peg.386
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK00128
- BioCyc:   LPLA220668:LP_0460-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32025; Mature: 32025

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVEKAPAKINLGLDTLFEHPNGDKEWDMVMTSVDLADYVMLESLHTNRIEVVTDSGFL
CCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PNDRRNLAFQAVSVLKRYCHVDRGVRIKIRKAIPVAAGLGGGSSDAAAVLRGLNRMWNLH
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHE
LDLATLARLGLQVDSDVPYCVYSQTAHVTGKGDVVTPLPKLPPMWIILAKPKVSVSTPNI
ECHHHHHHHCCEECCCCCEEEECCCEEECCCCCEECCCCCCCCEEEEEECCCCCCCCHHH
LRQVNYERIDQHPNIEALLAGIQQQDFAEIFANMGNVLEPITAKRYPEILQIKRQLLTFG
HHHCCHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
ADAAQMSGTGPTVFGVCRKQSRAQRVYNSLKGFCREVYLVRPVNLNEH
CCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure
MQIVEKAPAKINLGLDTLFEHPNGDKEWDMVMTSVDLADYVMLESLHTNRIEVVTDSGFL
CCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
PNDRRNLAFQAVSVLKRYCHVDRGVRIKIRKAIPVAAGLGGGSSDAAAVLRGLNRMWNLH
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHE
LDLATLARLGLQVDSDVPYCVYSQTAHVTGKGDVVTPLPKLPPMWIILAKPKVSVSTPNI
ECHHHHHHHCCEECCCCCEEEECCCEEECCCCCEECCCCCCCCEEEEEECCCCCCCCHHH
LRQVNYERIDQHPNIEALLAGIQQQDFAEIFANMGNVLEPITAKRYPEILQIKRQLLTFG
HHHCCHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
ADAAQMSGTGPTVFGVCRKQSRAQRVYNSLKGFCREVYLVRPVNLNEH
CCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566