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The map label for this gene is ack1 [H]

Identifier: 28377144

GI number: 28377144

Start: 193763

End: 194950

Strand: Direct

Name: ack1 [H]

Synonym: lp_0210

Alternate gene names: 28377144

Gene position: 193763-194950 (Clockwise)

Preceding gene: 28377142

Following gene: 28377148

Centisome position: 5.86

GC content: 45.37

Gene sequence:

>1188_bases
ATGCAAAAAACATTAATTATTAACGCAGGTAGTTCATCATTGAAATGGCAATTATTTGCTATGCCTGCTGAAACGGTGGT
TGCTAGTGGTCTCGTTGAACGGATTAGTATGCCCGGTTCAATTTTCACCATCAAGTATGGTGACCATCAAAAGTTTGAAA
CGACAGTCGATAATTTAGATCAAAATCATGCGGCGCAGATGTTATTGGCTGAATTACAGCGATTAGCAATTATTGACTCA
TTAACGGAAATTACGGCAGTTGCTCACCGAGTCGTTGCGGGTGGCGAAACGTTCAAGCAAGCAGTTGAAGTGACGCCAGC
GGTACTCGAGGCCATTAAGGGACTTAGTGACTTTGCACCATTGCATAATCCGATGGAGGCACGTGGTATTGAGACGATGG
CTCAGGCTTTACCGACCGTTAAACAGTACGCGGTATTTGATAGTCAATTCTTTACCGATTTACCAGAAATGAATGCAATT
TACAGTTTGCCATATGAATTGACCCAAAAATACCATATTCGCCGTTACGGTGAACATGGTATCTCGCATGGTTACTTAAC
CGGTCGGGCTGCAGAACTGCTGGCTAAGCCGAAAGATGACCTCAACCTGGTGACGTTACACTTAGGTAGTGGCGCGTCAT
TAGCTGCCGTAAAAAATGGCCGGGCCTTCGACACATCGATGGGCTTTACGCCATTAACGGGTGTCACAATGGGAACGCGC
GCGGGTGACGTTGATCCGGCCGTATTGCCATTCCTCATGAAAGCTTTAAAGATCGATGATCCGAATGAAATCATGATGAT
GCTTAACAATCAGTCTGGGTTGTTAGGAATCTCAGGAATTTCACCAGATATGCGTGAAATCCGTGCGCAAGAAGCCACGA
ATCCCCAAGCTCAACTAGCTGTTGAAATTTTTGTCAATCGGATTGTTAAGTATGCCGGTAGCTATTTGACAGAATTAAAA
CGGGCGGATGCGCTGATTTTTGCGGGCGGCATTGGCGAGCATAATGCGACCTTGCGCCAGCAAATTATTGATGAACTGAG
CATCTTTGGTGTGACCCTGGATCCGAAATTAAATGAAGCGGGTCAAGAAGGACTCATCAGTGGCGCAGATTCCAAGATTA
AAGTGATGTTGATCCCAACTAACGAAGAATTAGCGATGGTGCGACAAGTTGCGGCGTTGCAAAATTAA

Upstream 100 bases:

>100_bases
GGACTAGCGAATGAATTAAGATGTCAAATGACACGATTGAAAAAAGTTGAGCAATTTTCAGAAAAGTTCGGTATTCTATT
ACATGGGAGTTGATTGAGAA

Downstream 100 bases:

>100_bases
GCTTCTCTTAATTTACGACTGCAATCAGTCTGCCGCTGTATAGCGCGTGCTATACTAACTGGCAAGGTACCCATGATGGG
ACTTGTAAGGAGGGAAGCTC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MQKTLIINAGSSSLKWQLFAMPAETVVASGLVERISMPGSIFTIKYGDHQKFETTVDNLDQNHAAQMLLAELQRLAIIDS
LTEITAVAHRVVAGGETFKQAVEVTPAVLEAIKGLSDFAPLHNPMEARGIETMAQALPTVKQYAVFDSQFFTDLPEMNAI
YSLPYELTQKYHIRRYGEHGISHGYLTGRAAELLAKPKDDLNLVTLHLGSGASLAAVKNGRAFDTSMGFTPLTGVTMGTR
AGDVDPAVLPFLMKALKIDDPNEIMMMLNNQSGLLGISGISPDMREIRAQEATNPQAQLAVEIFVNRIVKYAGSYLTELK
RADALIFAGGIGEHNATLRQQIIDELSIFGVTLDPKLNEAGQEGLISGADSKIKVMLIPTNEELAMVRQVAALQN

Sequences:

>Translated_395_residues
MQKTLIINAGSSSLKWQLFAMPAETVVASGLVERISMPGSIFTIKYGDHQKFETTVDNLDQNHAAQMLLAELQRLAIIDS
LTEITAVAHRVVAGGETFKQAVEVTPAVLEAIKGLSDFAPLHNPMEARGIETMAQALPTVKQYAVFDSQFFTDLPEMNAI
YSLPYELTQKYHIRRYGEHGISHGYLTGRAAELLAKPKDDLNLVTLHLGSGASLAAVKNGRAFDTSMGFTPLTGVTMGTR
AGDVDPAVLPFLMKALKIDDPNEIMMMLNNQSGLLGISGISPDMREIRAQEATNPQAQLAVEIFVNRIVKYAGSYLTELK
RADALIFAGGIGEHNATLRQQIIDELSIFGVTLDPKLNEAGQEGLISGADSKIKVMLIPTNEELAMVRQVAALQN
>Mature_395_residues
MQKTLIINAGSSSLKWQLFAMPAETVVASGLVERISMPGSIFTIKYGDHQKFETTVDNLDQNHAAQMLLAELQRLAIIDS
LTEITAVAHRVVAGGETFKQAVEVTPAVLEAIKGLSDFAPLHNPMEARGIETMAQALPTVKQYAVFDSQFFTDLPEMNAI
YSLPYELTQKYHIRRYGEHGISHGYLTGRAAELLAKPKDDLNLVTLHLGSGASLAAVKNGRAFDTSMGFTPLTGVTMGTR
AGDVDPAVLPFLMKALKIDDPNEIMMMLNNQSGLLGISGISPDMREIRAQEATNPQAQLAVEIFVNRIVKYAGSYLTELK
RADALIFAGGIGEHNATLRQQIIDELSIFGVTLDPKLNEAGQEGLISGADSKIKVMLIPTNEELAMVRQVAALQN

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=37.5, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI145693184, Length=388, Percent_Identity=35.3092783505155, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 42893; Mature: 42893

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTLIINAGSSSLKWQLFAMPAETVVASGLVERISMPGSIFTIKYGDHQKFETTVDNLD
CCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCC
QNHAAQMLLAELQRLAIIDSLTEITAVAHRVVAGGETFKQAVEVTPAVLEAIKGLSDFAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
LHNPMEARGIETMAQALPTVKQYAVFDSQFFTDLPEMNAIYSLPYELTQKYHIRRYGEHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHHHHHHHHCCC
ISHGYLTGRAAELLAKPKDDLNLVTLHLGSGASLAAVKNGRAFDTSMGFTPLTGVTMGTR
CCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCEEECCCCCCCCCCCEECCC
AGDVDPAVLPFLMKALKIDDPNEIMMMLNNQSGLLGISGISPDMREIRAQEATNPQAQLA
CCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHCCCHHHHHH
VEIFVNRIVKYAGSYLTELKRADALIFAGGIGEHNATLRQQIIDELSIFGVTLDPKLNEA
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCCC
GQEGLISGADSKIKVMLIPTNEELAMVRQVAALQN
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKTLIINAGSSSLKWQLFAMPAETVVASGLVERISMPGSIFTIKYGDHQKFETTVDNLD
CCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCC
QNHAAQMLLAELQRLAIIDSLTEITAVAHRVVAGGETFKQAVEVTPAVLEAIKGLSDFAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
LHNPMEARGIETMAQALPTVKQYAVFDSQFFTDLPEMNAIYSLPYELTQKYHIRRYGEHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHHHHHHHHCCC
ISHGYLTGRAAELLAKPKDDLNLVTLHLGSGASLAAVKNGRAFDTSMGFTPLTGVTMGTR
CCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCEEECCCCCCCCCCCEECCC
AGDVDPAVLPFLMKALKIDDPNEIMMMLNNQSGLLGISGISPDMREIRAQEATNPQAQLA
CCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHCCCHHHHHH
VEIFVNRIVKYAGSYLTELKRADALIFAGGIGEHNATLRQQIIDELSIFGVTLDPKLNEA
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCCC
GQEGLISGADSKIKVMLIPTNEELAMVRQVAALQN
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]