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The map label for this gene is amy2 [H]

Identifier: 28377119

GI number: 28377119

Start: 155655

End: 156977

Strand: Direct

Name: amy2 [H]

Synonym: lp_0179

Alternate gene names: 28377119

Gene position: 155655-156977 (Clockwise)

Preceding gene: 28377118

Following gene: 28377120

Centisome position: 4.71

GC content: 45.58

Gene sequence:

>1323_bases
ATGGCACGCGATACGCAAACGCAATTACGCAATGAGATGATTTACTCAGTCTTTGTTAGAAACTACTCAGAAGCTGGTAA
TTTTGCTGGCGTAACTGCTGACTTACAACGAATTAAGGATTTAGGAACCGATATTTTGTGGCTACTGCCAATCAATCCGA
TCGGTGAGGTCAACCGTAAGGGGACACTTGGTTCGCCATATGCAATCAAGGACTACCGTGGGATCAATCCGGAATACGGG
ACTTTAGCTGACTTTAAAGCACTCACGGATAGGGCGCATGAATTAGGAATGAAAGTGATGTTAGACATTGTCTATAATCA
CACTTCGCCTGATTCAGTCTTAGCAACCGAACATCCAGAGTGGTTTTATCACGACGCGGATGGTCAGTTGACGAATAAGG
TCGGCGACTGGAGTGACGTTAAAGACTTAGACTATGGTCACCATGAGTTGTGGCAGTATCAAATCGACACACTCTTGTAT
TGGAGCCAGTTTGTGGACGGTTACCGTTGTGATGTTGCGCCATTAGTGCCACTTGATTTCTGGCTTGAAGCGCGGAAACA
GGTTAATGCGAAGTACCCGGAAACGTTATGGTTAGCGGAGTCAGCCGGCAGTGGTTTTATTGAGGAACTGCGGTCACAAG
GCTACACGGGTCTGTCTGACAGTGAACTTTATCAAGCTTTTGATATGACATACGATTACGACGTCTTCGGTGATTTTAAA
GATTACTGGCAAGGACGCAGTACGGTCGAACGGTATGTTGACTTGTTACAACGCCAAGATGCCACTTTCCCTGGTAATTA
TGTCAAGATGCGCTTCTTGGAAAATCATGATAATGCACGCATGATGAGCTTGATGCACAGCAAAGCTGAAGCCGTTAATA
ACTTGACCTGGATCTTCATGCAACGTGGTATTCCGTTAATCTATAACGGGCAAGAATTTTTGGCCGAACACCAACCATCA
TTGTTCGATCGGGACACCATGGTTGCAGATCGTCATGGGGACGTGACACCACTGATTCAAAAGCTAGTGACTATTAAGCA
ACTACCATTACTGCGTGCTGCGGACTACCAATTGGCAGTCGTAGAAGAAGGTATCGTTAAGATTACTTACCGTGCGGCTG
GCGAAGCGTTAACAGCCTGGATTCCGTTAAAAGGTCAGGTCACAGCAGTTGCGACTAAGCTAGCAGCTGGTAGCTATCAA
AATCTGTTAACAGATGGTCCTACTGAAGTTGTGGATGGCAAGCTGACAGTTGATGGTCAACCAGTATTAATTAAATATGT
CACCAATACGGCGGTGACTAAAGTTGCTGACCAGTCCAATTAG

Upstream 100 bases:

>100_bases
TGTGTTGTGGGTATATCTGAAAACAAAATTCAAAGATCCGGAAGTTGACCAAACCATGTTGCTTTAGGTCAATTAGTAAG
CATAGAAATGAGGAAGATTA

Downstream 100 bases:

>100_bases
CTGATTAATAGCTTTGAATAATCGATCAAAAAGCAACCGTTTGCGGGCGATTGCACGTGTAATAGTAGTTTAGAAGTGAG
AAAACAAGGGAGTGGATTTC

Product: alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MARDTQTQLRNEMIYSVFVRNYSEAGNFAGVTADLQRIKDLGTDILWLLPINPIGEVNRKGTLGSPYAIKDYRGINPEYG
TLADFKALTDRAHELGMKVMLDIVYNHTSPDSVLATEHPEWFYHDADGQLTNKVGDWSDVKDLDYGHHELWQYQIDTLLY
WSQFVDGYRCDVAPLVPLDFWLEARKQVNAKYPETLWLAESAGSGFIEELRSQGYTGLSDSELYQAFDMTYDYDVFGDFK
DYWQGRSTVERYVDLLQRQDATFPGNYVKMRFLENHDNARMMSLMHSKAEAVNNLTWIFMQRGIPLIYNGQEFLAEHQPS
LFDRDTMVADRHGDVTPLIQKLVTIKQLPLLRAADYQLAVVEEGIVKITYRAAGEALTAWIPLKGQVTAVATKLAAGSYQ
NLLTDGPTEVVDGKLTVDGQPVLIKYVTNTAVTKVADQSN

Sequences:

>Translated_440_residues
MARDTQTQLRNEMIYSVFVRNYSEAGNFAGVTADLQRIKDLGTDILWLLPINPIGEVNRKGTLGSPYAIKDYRGINPEYG
TLADFKALTDRAHELGMKVMLDIVYNHTSPDSVLATEHPEWFYHDADGQLTNKVGDWSDVKDLDYGHHELWQYQIDTLLY
WSQFVDGYRCDVAPLVPLDFWLEARKQVNAKYPETLWLAESAGSGFIEELRSQGYTGLSDSELYQAFDMTYDYDVFGDFK
DYWQGRSTVERYVDLLQRQDATFPGNYVKMRFLENHDNARMMSLMHSKAEAVNNLTWIFMQRGIPLIYNGQEFLAEHQPS
LFDRDTMVADRHGDVTPLIQKLVTIKQLPLLRAADYQLAVVEEGIVKITYRAAGEALTAWIPLKGQVTAVATKLAAGSYQ
NLLTDGPTEVVDGKLTVDGQPVLIKYVTNTAVTKVADQSN
>Mature_439_residues
ARDTQTQLRNEMIYSVFVRNYSEAGNFAGVTADLQRIKDLGTDILWLLPINPIGEVNRKGTLGSPYAIKDYRGINPEYGT
LADFKALTDRAHELGMKVMLDIVYNHTSPDSVLATEHPEWFYHDADGQLTNKVGDWSDVKDLDYGHHELWQYQIDTLLYW
SQFVDGYRCDVAPLVPLDFWLEARKQVNAKYPETLWLAESAGSGFIEELRSQGYTGLSDSELYQAFDMTYDYDVFGDFKD
YWQGRSTVERYVDLLQRQDATFPGNYVKMRFLENHDNARMMSLMHSKAEAVNNLTWIFMQRGIPLIYNGQEFLAEHQPSL
FDRDTMVADRHGDVTPLIQKLVTIKQLPLLRAADYQLAVVEEGIVKITYRAAGEALTAWIPLKGQVTAVATKLAAGSYQN
LLTDGPTEVVDGKLTVDGQPVLIKYVTNTAVTKVADQSN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1790687, Length=139, Percent_Identity=30.9352517985612, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=131, Percent_Identity=32.824427480916, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=131, Percent_Identity=32.824427480916, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=131, Percent_Identity=32.0610687022901, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=114, Percent_Identity=35.0877192982456, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583745, Length=381, Percent_Identity=25.7217847769029, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586599, Length=282, Percent_Identity=28.0141843971631, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586597, Length=210, Percent_Identity=29.0476190476191, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24583747, Length=394, Percent_Identity=23.3502538071066, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583749, Length=394, Percent_Identity=23.3502538071066, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549022, Length=204, Percent_Identity=26.9607843137255, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24586589, Length=123, Percent_Identity=32.520325203252, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586591, Length=123, Percent_Identity=30.0813008130081, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24586587, Length=211, Percent_Identity=26.0663507109005, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 49916; Mature: 49785

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTQTQLRNEMIYSVFVRNYSEAGNFAGVTADLQRIKDLGTDILWLLPINPIGEVNRK
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
GTLGSPYAIKDYRGINPEYGTLADFKALTDRAHELGMKVMLDIVYNHTSPDSVLATEHPE
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
WFYHDADGQLTNKVGDWSDVKDLDYGHHELWQYQIDTLLYWSQFVDGYRCDVAPLVPLDF
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
WLEARKQVNAKYPETLWLAESAGSGFIEELRSQGYTGLSDSELYQAFDMTYDYDVFGDFK
HHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHH
DYWQGRSTVERYVDLLQRQDATFPGNYVKMRFLENHDNARMMSLMHSKAEAVNNLTWIFM
HHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEE
QRGIPLIYNGQEFLAEHQPSLFDRDTMVADRHGDVTPLIQKLVTIKQLPLLRAADYQLAV
CCCCCEEECCHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEECCCEEEE
VEEGIVKITYRAAGEALTAWIPLKGQVTAVATKLAAGSYQNLLTDGPTEVVDGKLTVDGQ
EECCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHCCCEEEECCC
PVLIKYVTNTAVTKVADQSN
CEEEEEECCHHHHHHCCCCC
>Mature Secondary Structure 
ARDTQTQLRNEMIYSVFVRNYSEAGNFAGVTADLQRIKDLGTDILWLLPINPIGEVNRK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
GTLGSPYAIKDYRGINPEYGTLADFKALTDRAHELGMKVMLDIVYNHTSPDSVLATEHPE
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
WFYHDADGQLTNKVGDWSDVKDLDYGHHELWQYQIDTLLYWSQFVDGYRCDVAPLVPLDF
CEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
WLEARKQVNAKYPETLWLAESAGSGFIEELRSQGYTGLSDSELYQAFDMTYDYDVFGDFK
HHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHH
DYWQGRSTVERYVDLLQRQDATFPGNYVKMRFLENHDNARMMSLMHSKAEAVNNLTWIFM
HHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEE
QRGIPLIYNGQEFLAEHQPSLFDRDTMVADRHGDVTPLIQKLVTIKQLPLLRAADYQLAV
CCCCCEEECCHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEECCCEEEE
VEEGIVKITYRAAGEALTAWIPLKGQVTAVATKLAAGSYQNLLTDGPTEVVDGKLTVDGQ
EECCEEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHCCCEEEECCC
PVLIKYVTNTAVTKVADQSN
CEEEEEECCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2458257 [H]