Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 28377064

GI number: 28377064

Start: 101064

End: 101717

Strand: Direct

Name: thiE

Synonym: lp_0115

Alternate gene names: 28377064

Gene position: 101064-101717 (Clockwise)

Preceding gene: 28377063

Following gene: 28377065

Centisome position: 3.05

GC content: 52.45

Gene sequence:

>654_bases
ATGACGCTTAAATTTGAACCCACACAATTACGTGCTTATTTTGTGTGCGGGACACAAGACGTGCCGGGACAGGATCTAAA
TATGGTCGTCCAAACGGCCTTGGATGCAGGCGTCACGGCATTCCAATACCGAGATAAAGGCAATAGCCAGTTGACTACGG
TTGAGCGTTTTGCATTGGGGCAGCAGCTGCGGGAACGCTGTGCGCAAGCGCACGTTCCCTTTATCGTCGATGATGATGTC
GAGCTGGCACTCGCACTGCAAGCAGATGGGATTCACGTTGGTCAAAAGGACGACTGGGTGACACAAGTTATTCAACGGGT
CGCTAATCAAATGTTTGTCGGTTTGTCTTGTTCAACCTTAGCTGAAGTGCAGATTGCTAACCAATTGGAAGGCATTGCCT
ATCTTGGTAGTGGTCCCATCTTTCCAACGACATCTAAGGCCGATGCGGATCCAGTAGTCGGTCTGACGGGGTTGCGACAA
TTAGTCATGACTGCATCCTGTCCCGTTGTCGCCATTGGCGGAATTACTGTCGCGCAATTGCCAGCTATTGCGGCTACTGG
TGCGGCTGGCGCTGCGGTGATCTCAATGTTAACGCGTAGTCCCGATATGGCCGCCACGGTCAAGGCGATGCTAACGGCTA
CGGAGGGGCACTGA

Upstream 100 bases:

>100_bases
AAGGCGTACGTTGCTGGGACGATTCAGGATGGTATCCAGGTCGGGCATGGTCATGGCCCGTTGAATCATTGGGCAACCCC
AAGTGAGCAGGTGATCGTTC

Downstream 100 bases:

>100_bases
TGAAACATGACGGGATTTTTCGGAGCCAATTTTTATTGTGGCTCGGTGCGGCTATCTCAATTGCCGAAATCTTAACAGGT
ACGCTGATGGCACCACTGGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTLKFEPTQLRAYFVCGTQDVPGQDLNMVVQTALDAGVTAFQYRDKGNSQLTTVERFALGQQLRERCAQAHVPFIVDDDV
ELALALQADGIHVGQKDDWVTQVIQRVANQMFVGLSCSTLAEVQIANQLEGIAYLGSGPIFPTTSKADADPVVGLTGLRQ
LVMTASCPVVAIGGITVAQLPAIAATGAAGAAVISMLTRSPDMAATVKAMLTATEGH

Sequences:

>Translated_217_residues
MTLKFEPTQLRAYFVCGTQDVPGQDLNMVVQTALDAGVTAFQYRDKGNSQLTTVERFALGQQLRERCAQAHVPFIVDDDV
ELALALQADGIHVGQKDDWVTQVIQRVANQMFVGLSCSTLAEVQIANQLEGIAYLGSGPIFPTTSKADADPVVGLTGLRQ
LVMTASCPVVAIGGITVAQLPAIAATGAAGAAVISMLTRSPDMAATVKAMLTATEGH
>Mature_216_residues
TLKFEPTQLRAYFVCGTQDVPGQDLNMVVQTALDAGVTAFQYRDKGNSQLTTVERFALGQQLRERCAQAHVPFIVDDDVE
LALALQADGIHVGQKDDWVTQVIQRVANQMFVGLSCSTLAEVQIANQLEGIAYLGSGPIFPTTSKADADPVVGLTGLRQL
VMTASCPVVAIGGITVAQLPAIAATGAAGAAVISMLTRSPDMAATVKAMLTATEGH

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=179, Percent_Identity=32.4022346368715, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=207, Percent_Identity=27.0531400966184, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_LACPL (Q890C0)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783956.1
- ProteinModelPortal:   Q890C0
- SMR:   Q890C0
- GeneID:   1061356
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0115
- NMPDR:   fig|220668.1.peg.90
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   CLSK821159
- BioCyc:   LPLA220668:LP_0115-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22845; Mature: 22714

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKFEPTQLRAYFVCGTQDVPGQDLNMVVQTALDAGVTAFQYRDKGNSQLTTVERFALG
CCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
QQLRERCAQAHVPFIVDDDVELALALQADGIHVGQKDDWVTQVIQRVANQMFVGLSCSTL
HHHHHHHHHCCCCEEECCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHHEECCCHHHH
AEVQIANQLEGIAYLGSGPIFPTTSKADADPVVGLTGLRQLVMTASCPVVAIGGITVAQL
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHH
PAIAATGAAGAAVISMLTRSPDMAATVKAMLTATEGH
CHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHEECCCC
>Mature Secondary Structure 
TLKFEPTQLRAYFVCGTQDVPGQDLNMVVQTALDAGVTAFQYRDKGNSQLTTVERFALG
CCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
QQLRERCAQAHVPFIVDDDVELALALQADGIHVGQKDDWVTQVIQRVANQMFVGLSCSTL
HHHHHHHHHCCCCEEECCCCEEEEEEECCCEECCCCCHHHHHHHHHHHHHHEECCCHHHH
AEVQIANQLEGIAYLGSGPIFPTTSKADADPVVGLTGLRQLVMTASCPVVAIGGITVAQL
HHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHH
PAIAATGAAGAAVISMLTRSPDMAATVKAMLTATEGH
CHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566