Click here to switch to the map view.

The map label for this gene is glpF1 [H]

Identifier: 28377059

GI number: 28377059

Start: 96329

End: 97045

Strand: Direct

Name: glpF1 [H]

Synonym: lp_0108

Alternate gene names: 28377059

Gene position: 96329-97045 (Clockwise)

Preceding gene: 28377058

Following gene: 28377060

Centisome position: 2.91

GC content: 44.07

Gene sequence:

>717_bases
TTGGTACATCAGTTGATTGCTGAATTTATGGGCACAGCCCTAATGATTATTTTTGGGGTGGGTGTTCATTGTAGTTCCGT
ATTAAAGGGAACCAAGTATCGGGGTTCCGGTCATATTTTTGCCATTACAACTTGGGGTTTTGGGATTAGCGTTGCCTTAT
TCATTTTTGGCAATGTTTGCATTAACCCGGCAATGGTTTTGGCGCAGTGTCTATTAGGAAATATCGCATGGTCTTTATTT
ATTCCGTATTCCGTTGCGGAAGTGCTTGGTGGGGTCGTGGGCTCAGTGATCGTTTGGATCATGTATGCTGATCATTTTAA
AGCCTCAACAGATGAGATTTCACCCATTACAATTCGGAACTTATTCTGTACGGCACCAGCCGTTCGTAACTTACCGCGGA
ATTTCTTTGTGGAATTATTTGATACATTTATTTTTATTTCGGGTATTTTAGCGATTTCTGAGATTAAAACGCCGGGAATC
GTGCCAATTGGCGTTGGCCTACTTGTATGGGCCATTGGGATGGGACTTGGTGGTCCAACTGGGTTCGCAATGAACTTAGC
TCGAGATATGGGGCCCCGGATTGCCCACGCCATCTTACCAATTGCCAATAAAGCCGATAGTGATTGGCAATACGGTATCA
TTGTGCCAGGAATTGCGCCCTTTGTCGGGGCAGCAATTGCCGCTTGGTTTATGCATGGCTTTTTTGGAATTAATTAA

Upstream 100 bases:

>100_bases
GATTGTGCCCAGCTGGCGCAGCAATTTCATATTCCGTTTAGAACGGTCTACCAGGCAGCCTTAGTGGCGGTAGATCAATT
AGATGAGGAGGCGTAATTTT

Downstream 100 bases:

>100_bases
ATTAAATAGAAGAAGGGTTTTATTATGGCAACATTAGCAACAAAAAAAGCAACGTTAGTAGCAGCATTAAAGGATTTACA
ACGGGTCACCGTGGCCTTTT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MVHQLIAEFMGTALMIIFGVGVHCSSVLKGTKYRGSGHIFAITTWGFGISVALFIFGNVCINPAMVLAQCLLGNIAWSLF
IPYSVAEVLGGVVGSVIVWIMYADHFKASTDEISPITIRNLFCTAPAVRNLPRNFFVELFDTFIFISGILAISEIKTPGI
VPIGVGLLVWAIGMGLGGPTGFAMNLARDMGPRIAHAILPIANKADSDWQYGIIVPGIAPFVGAAIAAWFMHGFFGIN

Sequences:

>Translated_238_residues
MVHQLIAEFMGTALMIIFGVGVHCSSVLKGTKYRGSGHIFAITTWGFGISVALFIFGNVCINPAMVLAQCLLGNIAWSLF
IPYSVAEVLGGVVGSVIVWIMYADHFKASTDEISPITIRNLFCTAPAVRNLPRNFFVELFDTFIFISGILAISEIKTPGI
VPIGVGLLVWAIGMGLGGPTGFAMNLARDMGPRIAHAILPIANKADSDWQYGIIVPGIAPFVGAAIAAWFMHGFFGIN
>Mature_238_residues
MVHQLIAEFMGTALMIIFGVGVHCSSVLKGTKYRGSGHIFAITTWGFGISVALFIFGNVCINPAMVLAQCLLGNIAWSLF
IPYSVAEVLGGVVGSVIVWIMYADHFKASTDEISPITIRNLFCTAPAVRNLPRNFFVELFDTFIFISGILAISEIKTPGI
VPIGVGLLVWAIGMGLGGPTGFAMNLARDMGPRIAHAILPIANKADSDWQYGIIVPGIAPFVGAAIAAWFMHGFFGIN

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI22538420, Length=242, Percent_Identity=28.099173553719, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4826645, Length=238, Percent_Identity=30.2521008403361, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4502187, Length=236, Percent_Identity=29.6610169491525, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI157266307, Length=242, Percent_Identity=29.3388429752066, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790362, Length=251, Percent_Identity=30.6772908366534, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992966, Length=253, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994009, Length=256, Percent_Identity=26.5625, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531431, Length=254, Percent_Identity=28.3464566929134, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17531429, Length=254, Percent_Identity=28.3464566929134, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=218, Percent_Identity=29.8165137614679, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25450; Mature: 25450

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHQLIAEFMGTALMIIFGVGVHCSSVLKGTKYRGSGHIFAITTWGFGISVALFIFGNVC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
INPAMVLAQCLLGNIAWSLFIPYSVAEVLGGVVGSVIVWIMYADHFKASTDEISPITIRN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHH
LFCTAPAVRNLPRNFFVELFDTFIFISGILAISEIKTPGIVPIGVGLLVWAIGMGLGGPT
HHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
GFAMNLARDMGPRIAHAILPIANKADSDWQYGIIVPGIAPFVGAAIAAWFMHGFFGIN
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVHQLIAEFMGTALMIIFGVGVHCSSVLKGTKYRGSGHIFAITTWGFGISVALFIFGNVC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
INPAMVLAQCLLGNIAWSLFIPYSVAEVLGGVVGSVIVWIMYADHFKASTDEISPITIRN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHH
LFCTAPAVRNLPRNFFVELFDTFIFISGILAISEIKTPGIVPIGVGLLVWAIGMGLGGPT
HHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCC
GFAMNLARDMGPRIAHAILPIANKADSDWQYGIIVPGIAPFVGAAIAAWFMHGFFGIN
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]