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The map label for this gene is rlmH

Identifier: 28377010

GI number: 28377010

Start: 45638

End: 46117

Strand: Direct

Name: rlmH

Synonym: lp_0045

Alternate gene names: 28377010

Gene position: 45638-46117 (Clockwise)

Preceding gene: 28377009

Following gene: 28377011

Centisome position: 1.38

GC content: 43.33

Gene sequence:

>480_bases
ATGAACATCAAAATTATCTGTGTCGGGAAATTAAAAGAGAAATACTTCAAACAGGGGATTGCGGAATATGCGAAAAGAAT
GAGTAAATTTGCAAAATTCCAGATTGTTGAAGTGCCAGATGAAAAGGCTCCAGAGTCATTAAGTAACGCAGAGATGGAAA
ATGTGAAGGCCAAAGAGGGCCAACGGATCCTAGAAAAGATCAAGGATCGCGATTACGTATACGCGCTAGCAATTTTGGGC
AAAGAACGCTCGAGTGAAGAATTTGCGGCGGAAATTGATAAACTAACGACGTATGGTCACAGTGATATTGATTTCGTAAT
CGGTGGTTCGCTGGGATTATCACCGGAAGTGCTGAAGCGCGCGGATACTCAGATTTCGTTTGGGCGTTTCACGTTACCTC
ACCAATTAATGCGGTTAGTGCTGTCGGAGCAGGTTTATCGGGCGTTTATGATTAATGTGGGAAGTCCGTACCATAAGTGA

Upstream 100 bases:

>100_bases
ATAAATATTGTGGCGAACTGGTGTTCAACACAATATATTGATTATTGAGTTATGCACCTGTGGATAACTATGTTAATAAC
TTGTGTAAAGGCGGTCAATT

Downstream 100 bases:

>100_bases
TGCTAAAATTTGTTTTTCAATAGTTACGGATTTATTAGGTGAATAGGAATTCTAATCTGTTTTTGATGGTCGTATTTATA
ACAGTTTAGATAAGGCTGCA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNIKIICVGKLKEKYFKQGIAEYAKRMSKFAKFQIVEVPDEKAPESLSNAEMENVKAKEGQRILEKIKDRDYVYALAILG
KERSSEEFAAEIDKLTTYGHSDIDFVIGGSLGLSPEVLKRADTQISFGRFTLPHQLMRLVLSEQVYRAFMINVGSPYHK

Sequences:

>Translated_159_residues
MNIKIICVGKLKEKYFKQGIAEYAKRMSKFAKFQIVEVPDEKAPESLSNAEMENVKAKEGQRILEKIKDRDYVYALAILG
KERSSEEFAAEIDKLTTYGHSDIDFVIGGSLGLSPEVLKRADTQISFGRFTLPHQLMRLVLSEQVYRAFMINVGSPYHK
>Mature_159_residues
MNIKIICVGKLKEKYFKQGIAEYAKRMSKFAKFQIVEVPDEKAPESLSNAEMENVKAKEGQRILEKIKDRDYVYALAILG
KERSSEEFAAEIDKLTTYGHSDIDFVIGGSLGLSPEVLKRADTQISFGRFTLPHQLMRLVLSEQVYRAFMINVGSPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=31.4465408805031, Blast_Score=97, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_LACPL (Q890H4)

Other databases:

- EMBL:   AL935252
- RefSeq:   NP_783902.1
- ProteinModelPortal:   Q890H4
- SMR:   Q890H4
- GeneID:   1061237
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0045
- NMPDR:   fig|220668.1.peg.36
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   LPLA220668:LP_0045-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18109; Mature: 18109

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKIICVGKLKEKYFKQGIAEYAKRMSKFAKFQIVEVPDEKAPESLSNAEMENVKAKEG
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
QRILEKIKDRDYVYALAILGKERSSEEFAAEIDKLTTYGHSDIDFVIGGSLGLSPEVLKR
HHHHHHHCCCCCEEHEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
ADTQISFGRFTLPHQLMRLVLSEQVYRAFMINVGSPYHK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIKIICVGKLKEKYFKQGIAEYAKRMSKFAKFQIVEVPDEKAPESLSNAEMENVKAKEG
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
QRILEKIKDRDYVYALAILGKERSSEEFAAEIDKLTTYGHSDIDFVIGGSLGLSPEVLKR
HHHHHHHCCCCCEEHEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
ADTQISFGRFTLPHQLMRLVLSEQVYRAFMINVGSPYHK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566