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The map label for this gene is cspL

Identifier: 28377002

GI number: 28377002

Start: 36680

End: 36880

Strand: Direct

Name: cspL

Synonym: lp_0031

Alternate gene names: 28377002

Gene position: 36680-36880 (Clockwise)

Preceding gene: 28376998

Following gene: 28377003

Centisome position: 1.11

GC content: 40.8

Gene sequence:

>201_bases
ATGAAGAATGGTACAGTAAAATGGTTCAATGCGGATAAAGGGTTTGGTTTTATTACGGGTGAAGACGGTACCGATGTCTT
TGTTCATTTTTCAGCGATTCAAACTGATGGCTTTAAAACCTTAGATGAAGGTCAAAAAGTAACTTATGATGAAGAACAGG
GCGATCGTGGCCCCCAAGCAACGAACGTTCAACCACAATAG

Upstream 100 bases:

>100_bases
ATACTTAAGCCACTTAACGCGTGGAACGTTATGATTGATTGGCGCTCCCAAGGTTAACGATGTTGTTACTGCAGTCAGTT
CTGAGAGGACTTATCTAATA

Downstream 100 bases:

>100_bases
TAACTGTTGGGCCGAACCATTATCATTAACTGGTTCGGTTTTTATTTGAAGTGACGGGTTGTCAGCTAAACAAGAAAGCT
AAGTGGTCGGAATGGATTTT

Product: cold shock protein CspL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MKNGTVKWFNADKGFGFITGEDGTDVFVHFSAIQTDGFKTLDEGQKVTYDEEQGDRGPQATNVQPQ

Sequences:

>Translated_66_residues
MKNGTVKWFNADKGFGFITGEDGTDVFVHFSAIQTDGFKTLDEGQKVTYDEEQGDRGPQATNVQPQ
>Mature_66_residues
MKNGTVKWFNADKGFGFITGEDGTDVFVHFSAIQTDGFKTLDEGQKVTYDEEQGDRGPQATNVQPQ

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=72.1311475409836, Blast_Score=90, Evalue=2e-20,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=68.8524590163934, Blast_Score=87, Evalue=1e-19,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=68.8524590163934, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=68.8524590163934, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=65.5737704918033, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=62.2950819672131, Blast_Score=77, Evalue=1e-16,
Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=53.030303030303, Blast_Score=74, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSP2_LACPL (P96349)

Other databases:

- EMBL:   Y08940
- EMBL:   AL935252
- RefSeq:   NP_783894.1
- ProteinModelPortal:   P96349
- SMR:   P96349
- GeneID:   1061250
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0031
- HOGENOM:   HBG717773
- OMA:   FISAENG
- ProtClustDB:   CLSK272208
- BioCyc:   LPLA220668:LP_0031-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- PRINTS:   PR00050
- SMART:   SM00357

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 7300; Mature: 7300

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGTVKWFNADKGFGFITGEDGTDVFVHFSAIQTDGFKTLDEGQKVTYDEEQGDRGPQA
CCCCCEEEEECCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCC
TNVQPQ
CCCCCC
>Mature Secondary Structure
MKNGTVKWFNADKGFGFITGEDGTDVFVHFSAIQTDGFKTLDEGQKVTYDEEQGDRGPQA
CCCCCEEEEECCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCC
TNVQPQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9139925; 12566566