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The map label for this gene is dacB [H]

Identifier: 28211671

GI number: 28211671

Start: 2183257

End: 2184516

Strand: Direct

Name: dacB [H]

Synonym: CTC02051

Alternate gene names: 28211671

Gene position: 2183257-2184516 (Clockwise)

Preceding gene: 28211665

Following gene: 28211699

Centisome position: 77.99

GC content: 27.14

Gene sequence:

>1260_bases
ATGGTGTTAAGGAGGATAATTATGAAAAAATATATATTAACATTTTTAATATTTTTCCTTAGTTTCGTCTGTATTAATTC
TGCTACAATCAATGCTAAAACCTCAAAGCCACCTTCTGTATCTGCTGATGGGGCTATTTTAATGGATGCAAAAACTGGGA
AAGTATTATATGAAAAAAATATTAATAAGCCTTACCCTCCTGCCTCTACAACCAAAATAATGACAGCACTTCTTACTTTA
GAAAACTGTAACTTAGATGATGAAGTAGTAGTTGGTAAAAAACCTCCTTTAGCTGATGGAAGTAAAATATTTATTCATGA
AGGAGAAAAGCTAAAAGTAAAAGACTTATTATATGCTCTACTTTTAGAATCAGCTAATGATGCCGCTGAAGCTTTAGCTG
AACATATTGGCGGTTCTATAAATGATTTTTCTAAATTAATGAATAAAAGAGCCAAAGAACTTGGCTGTACTAATACTAAC
TTTGTTAATCCTAATGGTCTTTATAACGCTAAACATAGAACCACCGCACAGGATTTAGCTTTAATTATGAAAGAAGTTTC
TCAGTATGAAGATTATAGAAAAATATCTAATACTATTACATATAAGATAAAACCAACTAACAAAAGTAAAAAAGAAAGAC
CTTTGTGGAATAAAAATAAATTGGTTCAACAAAATTCTAAATATTATTTTAAAGGTTGTAATGGCGGTAAAACTGGTTAC
ACAACACAGTCACAACATTCTTATGTTGTAAGTGCTAATAGGGGCGACTTTCAACTTATAGTAGTATTAATACATGATAG
CAAAAAAACTTTTTTTGAAGATTCTGTAAACCTTTTGAACTATGGATTTAATAATTTTGAATTAATAAAAATGTATTCTA
GAGGTGATATTGTAACAGCATATAATGAGAAAGATTTAAGCTTAAATTTAATTGCCCGTGATGATTTTTATTATATAAAA
GAAAAGAATGATCCTTCAACGGCTATTTTAAATTTAGAAAAGAAAAATTTAAATTCAATTTCCTTTCATAAAGGAGATAC
TATCTTAAATGCTCAAGTTCTTTTAAATAATAATAATGTCGGCACATTGAATCTAGCTAGTGATCGTAATCATGAAACTT
CTATTTTTAAAATAAACTCTGAGAATAAATTAATTTTCTTTACCATTTTTGTAGTTTTATTTTTAATTTTTATGGTATCT
ATAATTAGATTTAGAAAAAAAGTGAAAAGAAAAAAACAGCAACGTAAGTATTTTTATTAA

Upstream 100 bases:

>100_bases
ATTTATATTACCTCCTTAGAAAGTTTTACTATTTCACATTATTGACAAATGCCTAAATAATTATCGCATTATAATTATTT
TTTTGATATAATTATTTTTA

Downstream 100 bases:

>100_bases
TTCCATAAAAGAAATCCATATTAGACCTTAACTCTAATATGGATTTCTTTTAATTATTCTCTCTTTTTTTAAAAATTTCC
ATTGAATCCTTAATATCAGA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MVLRRIIMKKYILTFLIFFLSFVCINSATINAKTSKPPSVSADGAILMDAKTGKVLYEKNINKPYPPASTTKIMTALLTL
ENCNLDDEVVVGKKPPLADGSKIFIHEGEKLKVKDLLYALLLESANDAAEALAEHIGGSINDFSKLMNKRAKELGCTNTN
FVNPNGLYNAKHRTTAQDLALIMKEVSQYEDYRKISNTITYKIKPTNKSKKERPLWNKNKLVQQNSKYYFKGCNGGKTGY
TTQSQHSYVVSANRGDFQLIVVLIHDSKKTFFEDSVNLLNYGFNNFELIKMYSRGDIVTAYNEKDLSLNLIARDDFYYIK
EKNDPSTAILNLEKKNLNSISFHKGDTILNAQVLLNNNNVGTLNLASDRNHETSIFKINSENKLIFFTIFVVLFLIFMVS
IIRFRKKVKRKKQQRKYFY

Sequences:

>Translated_419_residues
MVLRRIIMKKYILTFLIFFLSFVCINSATINAKTSKPPSVSADGAILMDAKTGKVLYEKNINKPYPPASTTKIMTALLTL
ENCNLDDEVVVGKKPPLADGSKIFIHEGEKLKVKDLLYALLLESANDAAEALAEHIGGSINDFSKLMNKRAKELGCTNTN
FVNPNGLYNAKHRTTAQDLALIMKEVSQYEDYRKISNTITYKIKPTNKSKKERPLWNKNKLVQQNSKYYFKGCNGGKTGY
TTQSQHSYVVSANRGDFQLIVVLIHDSKKTFFEDSVNLLNYGFNNFELIKMYSRGDIVTAYNEKDLSLNLIARDDFYYIK
EKNDPSTAILNLEKKNLNSISFHKGDTILNAQVLLNNNNVGTLNLASDRNHETSIFKINSENKLIFFTIFVVLFLIFMVS
IIRFRKKVKRKKQQRKYFY
>Mature_419_residues
MVLRRIIMKKYILTFLIFFLSFVCINSATINAKTSKPPSVSADGAILMDAKTGKVLYEKNINKPYPPASTTKIMTALLTL
ENCNLDDEVVVGKKPPLADGSKIFIHEGEKLKVKDLLYALLLESANDAAEALAEHIGGSINDFSKLMNKRAKELGCTNTN
FVNPNGLYNAKHRTTAQDLALIMKEVSQYEDYRKISNTITYKIKPTNKSKKERPLWNKNKLVQQNSKYYFKGCNGGKTGY
TTQSQHSYVVSANRGDFQLIVVLIHDSKKTFFEDSVNLLNYGFNNFELIKMYSRGDIVTAYNEKDLSLNLIARDDFYYIK
EKNDPSTAILNLEKKNLNSISFHKGDTILNAQVLLNNNNVGTLNLASDRNHETSIFKINSENKLIFFTIFVVLFLIFMVS
IIRFRKKVKRKKQQRKYFY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=285, Percent_Identity=35.7894736842105, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082022, Length=331, Percent_Identity=28.7009063444109, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786851, Length=299, Percent_Identity=30.1003344481605, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=29.5546558704453, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47812; Mature: 47812

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRRIIMKKYILTFLIFFLSFVCINSATINAKTSKPPSVSADGAILMDAKTGKVLYEKN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEECC
INKPYPPASTTKIMTALLTLENCNLDDEVVVGKKPPLADGSKIFIHEGEKLKVKDLLYAL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEECCCEEEHHHHHHHH
LLESANDAAEALAEHIGGSINDFSKLMNKRAKELGCTNTNFVNPNGLYNAKHRTTAQDLA
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
LIMKEVSQYEDYRKISNTITYKIKPTNKSKKERPLWNKNKLVQQNSKYYFKGCNGGKTGY
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCC
TTQSQHSYVVSANRGDFQLIVVLIHDSKKTFFEDSVNLLNYGFNNFELIKMYSRGDIVTA
CCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHCHHHHHCCCCCEEEEEEECCCCEEEE
YNEKDLSLNLIARDDFYYIKEKNDPSTAILNLEKKNLNSISFHKGDTILNAQVLLNNNNV
ECCCCCEEEEEEECCEEEEEECCCCCEEEEEEECCCCCEEEEECCCEEEEEEEEEECCCE
GTLNLASDRNHETSIFKINSENKLIFFTIFVVLFLIFMVSIIRFRKKVKRKKQQRKYFY
EEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVLRRIIMKKYILTFLIFFLSFVCINSATINAKTSKPPSVSADGAILMDAKTGKVLYEKN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEECC
INKPYPPASTTKIMTALLTLENCNLDDEVVVGKKPPLADGSKIFIHEGEKLKVKDLLYAL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEECCCEEEHHHHHHHH
LLESANDAAEALAEHIGGSINDFSKLMNKRAKELGCTNTNFVNPNGLYNAKHRTTAQDLA
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
LIMKEVSQYEDYRKISNTITYKIKPTNKSKKERPLWNKNKLVQQNSKYYFKGCNGGKTGY
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCC
TTQSQHSYVVSANRGDFQLIVVLIHDSKKTFFEDSVNLLNYGFNNFELIKMYSRGDIVTA
CCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHCHHHHHCCCCCEEEEEEECCCCEEEE
YNEKDLSLNLIARDDFYYIKEKNDPSTAILNLEKKNLNSISFHKGDTILNAQVLLNNNNV
ECCCCCEEEEEEECCEEEEEECCCCCEEEEEEECCCCCEEEEECCCEEEEEEEEEECCCE
GTLNLASDRNHETSIFKINSENKLIFFTIFVVLFLIFMVSIIRFRKKVKRKKQQRKYFY
EEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]