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The map label for this gene is ogt [H]

Identifier: 28211090

GI number: 28211090

Start: 1525054

End: 1525524

Strand: Direct

Name: ogt [H]

Synonym: CTC01413

Alternate gene names: 28211090

Gene position: 1525054-1525524 (Clockwise)

Preceding gene: 28211083

Following gene: 28211091

Centisome position: 54.48

GC content: 28.45

Gene sequence:

>471_bases
ATGAATAGCATATATTCCTACCAAACTGAAATAGGCAAAATTTATATAGTTGAAAATGGCATTGCAATTACCCATTTATA
TTTTAATGAATGTCCTATATTAGATAATTTTAAGAAAAAGGAAACTGAATTGATTAAAAAAGCTTATAACCAACTAAAGG
AGTATTTTTTAGGAAAGAGAAAAGAATTTGATCTTCCCCTTTTGCCTAAAGGTACAGATTTTCAACAAAAGGTTTGGAAA
GCATTAAGAGAAATTCCCTTTGGAGAAACTAAAAGTTATGGAGAAATTGCTAAAAATATTGGGAACCCTAAAGCTGCACG
TGCAGTTGGTATGGCTAATAATAAAAACCCTATCTCCATTTTTATTCCCTGTCATAGAGTAATTGGTTCAAATGGAAAAT
TAGTAGGATATGGTGGTGGACTAAAAATTAAAGAATACTTATTAAAAATTGAAAAAAATATAAATAGTTAA

Upstream 100 bases:

>100_bases
TTATTAATAAATTCTTAAATATATACTTTTCTAATTATTCATTTTAAAAATATAGTATTATATATATAAGATAAATAATG
AATATTGGGAGGTACGCCAA

Downstream 100 bases:

>100_bases
TAAAAAGGAGATAAACATTGAATAAAAAAATTAGAGAGGAAATTTTTGAATTAATAGATGAAGATTATAAGAAGTTTTCT
TCTTCTCTTTTACCTAATAT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNSIYSYQTEIGKIYIVENGIAITHLYFNECPILDNFKKKETELIKKAYNQLKEYFLGKRKEFDLPLLPKGTDFQQKVWK
ALREIPFGETKSYGEIAKNIGNPKAARAVGMANNKNPISIFIPCHRVIGSNGKLVGYGGGLKIKEYLLKIEKNINS

Sequences:

>Translated_156_residues
MNSIYSYQTEIGKIYIVENGIAITHLYFNECPILDNFKKKETELIKKAYNQLKEYFLGKRKEFDLPLLPKGTDFQQKVWK
ALREIPFGETKSYGEIAKNIGNPKAARAVGMANNKNPISIFIPCHRVIGSNGKLVGYGGGLKIKEYLLKIEKNINS
>Mature_156_residues
MNSIYSYQTEIGKIYIVENGIAITHLYFNECPILDNFKKKETELIKKAYNQLKEYFLGKRKEFDLPLLPKGTDFQQKVWK
ALREIPFGETKSYGEIAKNIGNPKAARAVGMANNKNPISIFIPCHRVIGSNGKLVGYGGGLKIKEYLLKIEKNINS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=97, Percent_Identity=48.4536082474227, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=54.3689320388349, Blast_Score=121, Evalue=2e-29,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=52.8089887640449, Blast_Score=99, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=52.4390243902439, Blast_Score=95, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17137554, Length=116, Percent_Identity=39.6551724137931, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17732; Mature: 17732

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00374 MGMT ; PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIYSYQTEIGKIYIVENGIAITHLYFNECPILDNFKKKETELIKKAYNQLKEYFLGKR
CCCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KEFDLPLLPKGTDFQQKVWKALREIPFGETKSYGEIAKNIGNPKAARAVGMANNKNPISI
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHEECCCCCCCCEEE
FIPCHRVIGSNGKLVGYGGGLKIKEYLLKIEKNINS
EEECHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSIYSYQTEIGKIYIVENGIAITHLYFNECPILDNFKKKETELIKKAYNQLKEYFLGKR
CCCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KEFDLPLLPKGTDFQQKVWKALREIPFGETKSYGEIAKNIGNPKAARAVGMANNKNPISI
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHEECCCCCCCCEEE
FIPCHRVIGSNGKLVGYGGGLKIKEYLLKIEKNINS
EEECHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]