Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 28210941

GI number: 28210941

Start: 1343746

End: 1344561

Strand: Direct

Name: rnhB

Synonym: CTC01255

Alternate gene names: 28210941

Gene position: 1343746-1344561 (Clockwise)

Preceding gene: 28210940

Following gene: 28210943

Centisome position: 48.0

GC content: 27.08

Gene sequence:

>816_bases
ATGGAGAAAGAAATTTATAAAATGGACTTTAATGATATGACTTTTAAAGATATAAAAGATATTGTAGATGGAATGAAGAA
AGTTGAAAATATAGAATGTTTAATATATATAAATGATATTCTAATGAAAGATAAAAGAAAAACAGTGCAAAATTTAAGTC
AGAGCATTAATAAATTTATATGTAGAAGAGAAGAAGAAATAGTAAGATTAAAAAAAATGTATGATTTTGATAAAAAATTT
GCTAATAATGTTCTAATTGCAGGAGTAGATGAGGTAGGAAGAGGACCATTAGCAGGACCAATCGTTGCAGCAGCTGTAAT
TTTAGATTTAAACTATAAAAACCATAATGATTTGTTTTATGGTTTGAAAGATTCCAAAAAGCTTAGAGCAAAGGAAAGGG
AGAATTTATCTCATATAATAAAGGATAAAGCTCTATACTATAATATATTTGAGTTAGACAATCATATGATAGACACTAGT
GGAATTGCTTGGTGCAATAATGAAGTTTTAAAAAAATCTACACTGGGTTTAGAGGTGACTCCTGGAATTGTAATATCTGA
TGGATTTAGCATAAAAAATATAAATATAAAAAATGAATACATAATAAAAGGAGATGCTAAAAGTGCAAGTATTGCAGCAG
CATCTATTATAGCAAAAGTATATAGAGATAAAAAAATGGAGGAGTACTCTAAAATATATCCTCATTACGGATTTGAAAAA
AATTCTGGTTATGGAACAGATGAACACACAAAGGCATTAAAAAAATACGGACCTTGCCCTATCCATAGAATGAGTTTTTT
ATCTAATTATATTTAA

Upstream 100 bases:

>100_bases
TAGATTATAATAGAGTAGCTATAATGATTTTAGATGAGTTTAGAGGTGGAAAACTGGGAAGGATATCTCTGGAAAAACCA
GAATAGGTAAGGGAGATAAT

Downstream 100 bases:

>100_bases
TAGCTGGAGCTAAAAATTTTAGAGTTGAAATGCATCTTTAATTAATCTAATAGAATAGTCATCATTATAATTATTAAAGA
TAATTTCAATTACATCAAAT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MEKEIYKMDFNDMTFKDIKDIVDGMKKVENIECLIYINDILMKDKRKTVQNLSQSINKFICRREEEIVRLKKMYDFDKKF
ANNVLIAGVDEVGRGPLAGPIVAAAVILDLNYKNHNDLFYGLKDSKKLRAKERENLSHIIKDKALYYNIFELDNHMIDTS
GIAWCNNEVLKKSTLGLEVTPGIVISDGFSIKNINIKNEYIIKGDAKSASIAAASIIAKVYRDKKMEEYSKIYPHYGFEK
NSGYGTDEHTKALKKYGPCPIHRMSFLSNYI

Sequences:

>Translated_271_residues
MEKEIYKMDFNDMTFKDIKDIVDGMKKVENIECLIYINDILMKDKRKTVQNLSQSINKFICRREEEIVRLKKMYDFDKKF
ANNVLIAGVDEVGRGPLAGPIVAAAVILDLNYKNHNDLFYGLKDSKKLRAKERENLSHIIKDKALYYNIFELDNHMIDTS
GIAWCNNEVLKKSTLGLEVTPGIVISDGFSIKNINIKNEYIIKGDAKSASIAAASIIAKVYRDKKMEEYSKIYPHYGFEK
NSGYGTDEHTKALKKYGPCPIHRMSFLSNYI
>Mature_271_residues
MEKEIYKMDFNDMTFKDIKDIVDGMKKVENIECLIYINDILMKDKRKTVQNLSQSINKFICRREEEIVRLKKMYDFDKKF
ANNVLIAGVDEVGRGPLAGPIVAAAVILDLNYKNHNDLFYGLKDSKKLRAKERENLSHIIKDKALYYNIFELDNHMIDTS
GIAWCNNEVLKKSTLGLEVTPGIVISDGFSIKNINIKNEYIIKGDAKSASIAAASIIAKVYRDKKMEEYSKIYPHYGFEK
NSGYGTDEHTKALKKYGPCPIHRMSFLSNYI

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=187, Percent_Identity=39.0374331550802, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CLOTE (Q895L8)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781885.1
- ProteinModelPortal:   Q895L8
- SMR:   Q895L8
- GeneID:   1059539
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01255
- NMPDR:   fig|212717.1.peg.1107
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   CTET212717:CTC_01255-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 31132; Mature: 31132

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEIYKMDFNDMTFKDIKDIVDGMKKVENIECLIYINDILMKDKRKTVQNLSQSINKFI
CCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
CRREEEIVRLKKMYDFDKKFANNVLIAGVDEVGRGPLAGPIVAAAVILDLNYKNHNDLFY
HCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEE
GLKDSKKLRAKERENLSHIIKDKALYYNIFELDNHMIDTSGIAWCNNEVLKKSTLGLEVT
ECCCCHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCEECCCCCEECCCHHHHHHCCCEEEC
PGIVISDGFSIKNINIKNEYIIKGDAKSASIAAASIIAKVYRDKKMEEYSKIYPHYGFEK
CCEEEECCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NSGYGTDEHTKALKKYGPCPIHRMSFLSNYI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEKEIYKMDFNDMTFKDIKDIVDGMKKVENIECLIYINDILMKDKRKTVQNLSQSINKFI
CCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
CRREEEIVRLKKMYDFDKKFANNVLIAGVDEVGRGPLAGPIVAAAVILDLNYKNHNDLFY
HCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEE
GLKDSKKLRAKERENLSHIIKDKALYYNIFELDNHMIDTSGIAWCNNEVLKKSTLGLEVT
ECCCCHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCEECCCCCEECCCHHHHHHCCCEEEC
PGIVISDGFSIKNINIKNEYIIKGDAKSASIAAASIIAKVYRDKKMEEYSKIYPHYGFEK
CCEEEECCEEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NSGYGTDEHTKALKKYGPCPIHRMSFLSNYI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129