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The map label for this gene is fmt

Identifier: 28210908

GI number: 28210908

Start: 1307532

End: 1308464

Strand: Direct

Name: fmt

Synonym: CTC01220

Alternate gene names: 28210908

Gene position: 1307532-1308464 (Clockwise)

Preceding gene: 28210907

Following gene: 28210909

Centisome position: 46.71

GC content: 28.62

Gene sequence:

>933_bases
TTGATGAAAATAGTATTTATGGGCACACCTGAATTTGCAGTACCATCTTTAGAAAGGCTTATTAAAGAATTTGGTGTTAA
AGCTGTTTTCACTCAACCAGATAGACCTAAAGGAAGAGGTAAAAAACTCTCAATATCCCCTATAAAAGAAGTTGCACTAA
GAGAAAATATAAAAATTTTACAACCACAAAAATTAAGAGATGATAGGGAAGCGATAGAATTTTTAAAAAAATTATCACCA
GATTTTATTATAGTAGTAGCCTATGGACAAATTCTATCCAAGGAGATATTAGATATTCCTAAATATGGATGCATAAATTT
ACATGCATCATTATTACCTAAATACAGAGGAGCAGCTCCTATAAATTGGGCCATAATAAATGGAGAAAAATTTAGTGGTA
ATACAACTATGTTTATGGATGTAGGTTTAGATACAGGAGATATGCTATTAAAAGATGAATTTAAAATAGAAGATAATACT
ACAGCAGGAGAACTTCATAATAAATTAATGGAAAGTGGAGGAGAACTTTTAGTAAAAACTATAAATGGATTAGTAGAGGA
TAGTATAGAACCTGAAAAACAAAAGGACGAAGAGTCTTGCTATGCATCCATGTTAGATAAAAAAATGGCTAAAATTAATT
GGAATTTAGAAAGTGAAGATATAAAAAATCTTATAAAGGGATTAAATCCATGGCCTTTAGCCTATACTTATTATAAGGGA
ACTATGATGAAAATATACGAAGCAGAAATAATAGATAAAAAAGAAGATTTTTCACCAGGTTTTATAATAGATGTATCAAA
AGGAGGAATAAAAGTTGCTACTAAAGATGGAATATTATTATTGAAAAAAATACAGTTCCCATCTAAAAAACCTATGTATG
TAGAAGAGTATATAAGAGGTAATAAAATAGAAAAAGGGATTATATTAGAGTAA

Upstream 100 bases:

>100_bases
GAAGGAGAGGAATTTTTAGCAAAGGCTCTATGCCATGAAATAGATCATTTAAATGGAGTTCTATTTGTAGATAAAATAAT
AGATGTGAAGGGGGAATAAT

Downstream 100 bases:

>100_bases
AAAGGAGGGATATGTATGTTCTATTATGATAGTACTATGATAATTCTAATACCTGCACTTATAATATCTTTCATAGCTCA
AATGAAAGTAAATAGTACTT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MMKIVFMGTPEFAVPSLERLIKEFGVKAVFTQPDRPKGRGKKLSISPIKEVALRENIKILQPQKLRDDREAIEFLKKLSP
DFIIVVAYGQILSKEILDIPKYGCINLHASLLPKYRGAAPINWAIINGEKFSGNTTMFMDVGLDTGDMLLKDEFKIEDNT
TAGELHNKLMESGGELLVKTINGLVEDSIEPEKQKDEESCYASMLDKKMAKINWNLESEDIKNLIKGLNPWPLAYTYYKG
TMMKIYEAEIIDKKEDFSPGFIIDVSKGGIKVATKDGILLLKKIQFPSKKPMYVEEYIRGNKIEKGIILE

Sequences:

>Translated_310_residues
MMKIVFMGTPEFAVPSLERLIKEFGVKAVFTQPDRPKGRGKKLSISPIKEVALRENIKILQPQKLRDDREAIEFLKKLSP
DFIIVVAYGQILSKEILDIPKYGCINLHASLLPKYRGAAPINWAIINGEKFSGNTTMFMDVGLDTGDMLLKDEFKIEDNT
TAGELHNKLMESGGELLVKTINGLVEDSIEPEKQKDEESCYASMLDKKMAKINWNLESEDIKNLIKGLNPWPLAYTYYKG
TMMKIYEAEIIDKKEDFSPGFIIDVSKGGIKVATKDGILLLKKIQFPSKKPMYVEEYIRGNKIEKGIILE
>Mature_310_residues
MMKIVFMGTPEFAVPSLERLIKEFGVKAVFTQPDRPKGRGKKLSISPIKEVALRENIKILQPQKLRDDREAIEFLKKLSP
DFIIVVAYGQILSKEILDIPKYGCINLHASLLPKYRGAAPINWAIINGEKFSGNTTMFMDVGLDTGDMLLKDEFKIEDNT
TAGELHNKLMESGGELLVKTINGLVEDSIEPEKQKDEESCYASMLDKKMAKINWNLESEDIKNLIKGLNPWPLAYTYYKG
TMMKIYEAEIIDKKEDFSPGFIIDVSKGGIKVATKDGILLLKKIQFPSKKPMYVEEYIRGNKIEKGIILE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=316, Percent_Identity=28.1645569620253, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI238814322, Length=315, Percent_Identity=28.2539682539683, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI164663775, Length=320, Percent_Identity=25, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789683, Length=302, Percent_Identity=41.7218543046358, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=27.4021352313167, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI133930964, Length=297, Percent_Identity=27.6094276094276, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45550868, Length=304, Percent_Identity=29.2763157894737, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585660, Length=241, Percent_Identity=26.1410788381743, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_CLOTE (Q895Q1)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781852.1
- ProteinModelPortal:   Q895Q1
- SMR:   Q895Q1
- GeneID:   1059545
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01220
- NMPDR:   fig|212717.1.peg.1074
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q895Q1
- ProtClustDB:   PRK00005
- BioCyc:   CTET212717:CTC_01220-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35099; Mature: 35099

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIVFMGTPEFAVPSLERLIKEFGVKAVFTQPDRPKGRGKKLSISPIKEVALRENIKIL
CEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCHHHHHHHHCCCEEE
QPQKLRDDREAIEFLKKLSPDFIIVVAYGQILSKEILDIPKYGCINLHASLLPKYRGAAP
CCHHCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHCCHHCCCCC
INWAIINGEKFSGNTTMFMDVGLDTGDMLLKDEFKIEDNTTAGELHNKLMESGGELLVKT
EEEEEECCCEECCCEEEEEEECCCCCCEEEECCEEECCCCCHHHHHHHHHHCCCHHHHHH
INGLVEDSIEPEKQKDEESCYASMLDKKMAKINWNLESEDIKNLIKGLNPWPLAYTYYKG
HHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCEEEEEECC
TMMKIYEAEIIDKKEDFSPGFIIDVSKGGIKVATKDGILLLKKIQFPSKKPMYVEEYIRG
CEEEEEHHHHCCCCCCCCCCEEEEECCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHCC
NKIEKGIILE
CCCCCCCCCC
>Mature Secondary Structure
MMKIVFMGTPEFAVPSLERLIKEFGVKAVFTQPDRPKGRGKKLSISPIKEVALRENIKIL
CEEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCHHHHHHHHCCCEEE
QPQKLRDDREAIEFLKKLSPDFIIVVAYGQILSKEILDIPKYGCINLHASLLPKYRGAAP
CCHHCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEHHHHCCHHCCCCC
INWAIINGEKFSGNTTMFMDVGLDTGDMLLKDEFKIEDNTTAGELHNKLMESGGELLVKT
EEEEEECCCEECCCEEEEEEECCCCCCEEEECCEEECCCCCHHHHHHHHHHCCCHHHHHH
INGLVEDSIEPEKQKDEESCYASMLDKKMAKINWNLESEDIKNLIKGLNPWPLAYTYYKG
HHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCEEEEEECC
TMMKIYEAEIIDKKEDFSPGFIIDVSKGGIKVATKDGILLLKKIQFPSKKPMYVEEYIRG
CEEEEEHHHHCCCCCCCCCCEEEEECCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHCC
NKIEKGIILE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129