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The map label for this gene is dut

Identifier: 28210898

GI number: 28210898

Start: 1296538

End: 1296975

Strand: Direct

Name: dut

Synonym: CTC01210

Alternate gene names: 28210898

Gene position: 1296538-1296975 (Clockwise)

Preceding gene: 28210897

Following gene: 28210899

Centisome position: 46.32

GC content: 31.28

Gene sequence:

>438_bases
ATGACAGAATATACATTAAAAATAAAATTATTAAAAGAAGATGCAAAATTACCTCAATATGCCCATGATAATGATGCGGG
AATGGATTTATTTTCTACAGAGGATAAAATTATAAATCCAGGAAAGCATGCACTCATTTTAACGGGAATAAGTATAGAAT
TACCTGAAAATACAGAAGCACAAATAAGACCAAGAAGTGGCTTAGCTCTAAAAAATGGCGTTACTGTATTGAATACTCCA
GGAACTATAGATGCAGGATATAGGGGAGAAATAGGAATTATATTGATTAACCATGGTGAGAAAAATTTTCAAGTGGAAAA
GGGTATGAAAATTGCTCAAATGGTTATAAAGCCAATTGTTAAAGTTAATATTGAAGAAGTAAAAATGTTATCTGAAAGTC
AAAGAGGCAAAGGTGGATTTGGCTCTACAGGAAAATAA

Upstream 100 bases:

>100_bases
TATTGGAGCTACTTTAGAAAAAGAAATTGATGAAGATACGGGAATTAAAATATTAAACTTTTAATATAAAATTATAATTT
TATAACTTAGGAGGAGTATA

Downstream 100 bases:

>100_bases
AGCATATAAATATGAGAAGGTTATTTTATTTAATTTATATTTAGTTTTAGAATGTTAAGGGGGAGACAGTGGTGAAGTTG
CGTAAAAATCTAAAATTGGC

Product: deoxyuridine 5-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTEYTLKIKLLKEDAKLPQYAHDNDAGMDLFSTEDKIINPGKHALILTGISIELPENTEAQIRPRSGLALKNGVTVLNTP
GTIDAGYRGEIGIILINHGEKNFQVEKGMKIAQMVIKPIVKVNIEEVKMLSESQRGKGGFGSTGK

Sequences:

>Translated_145_residues
MTEYTLKIKLLKEDAKLPQYAHDNDAGMDLFSTEDKIINPGKHALILTGISIELPENTEAQIRPRSGLALKNGVTVLNTP
GTIDAGYRGEIGIILINHGEKNFQVEKGMKIAQMVIKPIVKVNIEEVKMLSESQRGKGGFGSTGK
>Mature_144_residues
TEYTLKIKLLKEDAKLPQYAHDNDAGMDLFSTEDKIINPGKHALILTGISIELPENTEAQIRPRSGLALKNGVTVLNTPG
TIDAGYRGEIGIILINHGEKNFQVEKGMKIAQMVIKPIVKVNIEEVKMLSESQRGKGGFGSTGK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=42.8571428571429, Blast_Score=116, Evalue=9e-27,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=42.8571428571429, Blast_Score=116, Evalue=9e-27,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=42.8571428571429, Blast_Score=115, Evalue=2e-26,
Organism=Escherichia coli, GI1790071, Length=132, Percent_Identity=35.6060606060606, Blast_Score=92, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988561, Length=144, Percent_Identity=39.5833333333333, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319729, Length=144, Percent_Identity=38.1944444444444, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24583610, Length=140, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19921126, Length=140, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CLOTE (Q895R1)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781842.1
- ProteinModelPortal:   Q895R1
- SMR:   Q895R1
- GeneID:   1059158
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01210
- NMPDR:   fig|212717.1.peg.1064
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- PhylomeDB:   Q895R1
- BioCyc:   CTET212717:CTC_01210-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15830; Mature: 15698

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: BINDING 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYTLKIKLLKEDAKLPQYAHDNDAGMDLFSTEDKIINPGKHALILTGISIELPENTEA
CCCEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEECCCCCEEEEEEEEEECCCCCCE
QIRPRSGLALKNGVTVLNTPGTIDAGYRGEIGIILINHGEKNFQVEKGMKIAQMVIKPIV
EEECCCCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEHHCCHHHHHHHHHHHH
KVNIEEVKMLSESQRGKGGFGSTGK
HCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TEYTLKIKLLKEDAKLPQYAHDNDAGMDLFSTEDKIINPGKHALILTGISIELPENTEA
CCEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCCEECCCCCEEEEEEEEEECCCCCCE
QIRPRSGLALKNGVTVLNTPGTIDAGYRGEIGIILINHGEKNFQVEKGMKIAQMVIKPIV
EEECCCCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEHHCCHHHHHHHHHHHH
KVNIEEVKMLSESQRGKGGFGSTGK
HCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129