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The map label for this gene is afr [H]

Identifier: 28210621

GI number: 28210621

Start: 964188

End: 965216

Strand: Direct

Name: afr [H]

Synonym: CTC00910

Alternate gene names: 28210621

Gene position: 964188-965216 (Clockwise)

Preceding gene: 28210620

Following gene: 28210622

Centisome position: 34.44

GC content: 28.09

Gene sequence:

>1029_bases
ATGAAATTAAGATGGGGTATCATTGGTGCAAGTGGTATAGCTGATGAATGCACTATACCTGGAATATTAAAAACTAATAA
TTCAGAAGTAGTTGCAGTTATGAGTAGAAGTATAGAAAAAGCTGAAAAAATTAGAGAAAAATATAATATAAAAAAAGTAT
ATTCAAATGTAGAAGATTTATTGAATGATAAAGAGGTTGATGCAGTATATATTGCAACACCTGTTTTTGATCATAAAAAT
AGTGTTATTTTATCTGCAAAGGCTAAAAAACATATATTTCTTGAAAAACCTATGGGATTAACTACAGAAGAGTGCGAAGA
AATGATAAAGGTTACAAAAGAAAATGACGTTAAATTAGGAATTGCTTTTATGATGAGATTTGGGGAACATCATAGAAAAG
TTAGAAAGTTATTAAAAGAAAATATAATAGGAGATATTATTAATACAAGAATACAATATAGTTGGTGGTATCCAGATGAT
AATGTTTGGAGGCTTGATCCAAAATTAAGTGGTGGTGGTACAGTAGTAGATTTAGGCAGCCATTGCATTGACTTAATACA
ATACTTATTAGATACCAAAATAAAAACTGTTTCAGCAATGCTTGGAACAAAAACTTTTAACTATGAAGTTGAAGATTCAG
CTCAAATAATGTGTGAGATGGAAAATGGAGTATATGGTGCTTTTAATTTTCACTTTAATATTCCAAATAGTATATCAAAT
AATACTATGGAAATTTACGGTACAAAAGGTTCTATAGTTTGTTATGACACAATTGGAAGAGAAGATGTAGGTAAAGTTTT
AATTAAATTATCTGGAGAAAATGGAGAAATAGATTTCTCAGATTATACTTTAGAAGAACTATCTATAAATCCAGTTGACC
GTTATGAGAAAGAAATTCAAGCTTTTTCAGATGCAGTTATAAACAATGAAGAATTACCTGTTTCAGGTGAAGCAGGTTTA
AATGTAGTTAGAATAACTTCAGCTATTTACAAATCTTATAAAAATAAAAGATTTGTAAATGTTTATTAA

Upstream 100 bases:

>100_bases
GAAAGAAAATAGATAGTACGATAAAGTAAAAACTCACATTTATAAATGTGAGTTTTTATATAAGAAAAGAGTTATTAAAT
TATATAAGGGAGGGATTTAA

Downstream 100 bases:

>100_bases
TAATAAAACAGAGGTATTAATATGAAGGATTATAAAAAGATGTTAAAAGACTTAGAACAACAAGAAAAAGAGTTAGTATT
TTCTGAGTTTACAAATGAAA

Product: oxidoreductase, putative glucose--fructose oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKLRWGIIGASGIADECTIPGILKTNNSEVVAVMSRSIEKAEKIREKYNIKKVYSNVEDLLNDKEVDAVYIATPVFDHKN
SVILSAKAKKHIFLEKPMGLTTEECEEMIKVTKENDVKLGIAFMMRFGEHHRKVRKLLKENIIGDIINTRIQYSWWYPDD
NVWRLDPKLSGGGTVVDLGSHCIDLIQYLLDTKIKTVSAMLGTKTFNYEVEDSAQIMCEMENGVYGAFNFHFNIPNSISN
NTMEIYGTKGSIVCYDTIGREDVGKVLIKLSGENGEIDFSDYTLEELSINPVDRYEKEIQAFSDAVINNEELPVSGEAGL
NVVRITSAIYKSYKNKRFVNVY

Sequences:

>Translated_342_residues
MKLRWGIIGASGIADECTIPGILKTNNSEVVAVMSRSIEKAEKIREKYNIKKVYSNVEDLLNDKEVDAVYIATPVFDHKN
SVILSAKAKKHIFLEKPMGLTTEECEEMIKVTKENDVKLGIAFMMRFGEHHRKVRKLLKENIIGDIINTRIQYSWWYPDD
NVWRLDPKLSGGGTVVDLGSHCIDLIQYLLDTKIKTVSAMLGTKTFNYEVEDSAQIMCEMENGVYGAFNFHFNIPNSISN
NTMEIYGTKGSIVCYDTIGREDVGKVLIKLSGENGEIDFSDYTLEELSINPVDRYEKEIQAFSDAVINNEELPVSGEAGL
NVVRITSAIYKSYKNKRFVNVY
>Mature_342_residues
MKLRWGIIGASGIADECTIPGILKTNNSEVVAVMSRSIEKAEKIREKYNIKKVYSNVEDLLNDKEVDAVYIATPVFDHKN
SVILSAKAKKHIFLEKPMGLTTEECEEMIKVTKENDVKLGIAFMMRFGEHHRKVRKLLKENIIGDIINTRIQYSWWYPDD
NVWRLDPKLSGGGTVVDLGSHCIDLIQYLLDTKIKTVSAMLGTKTFNYEVEDSAQIMCEMENGVYGAFNFHFNIPNSISN
NTMEIYGTKGSIVCYDTIGREDVGKVLIKLSGENGEIDFSDYTLEELSINPVDRYEKEIQAFSDAVINNEELPVSGEAGL
NVVRITSAIYKSYKNKRFVNVY

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=253, Percent_Identity=26.8774703557312, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1787574, Length=350, Percent_Identity=25.7142857142857, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87082405, Length=206, Percent_Identity=33.0097087378641, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693182, Length=258, Percent_Identity=23.6434108527132, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581117, Length=256, Percent_Identity=26.5625, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920536, Length=266, Percent_Identity=23.6842105263158, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24581115, Length=189, Percent_Identity=26.984126984127, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 38713; Mature: 38713

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRWGIIGASGIADECTIPGILKTNNSEVVAVMSRSIEKAEKIREKYNIKKVYSNVEDL
CEEEEEEEECCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNDKEVDAVYIATPVFDHKNSVILSAKAKKHIFLEKPMGLTTEECEEMIKVTKENDVKLG
CCCCCCCEEEEEECEECCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHH
IAFMMRFGEHHRKVRKLLKENIIGDIINTRIQYSWWYPDDNVWRLDPKLSGGGTVVDLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCEEEEHHH
HCIDLIQYLLDTKIKTVSAMLGTKTFNYEVEDSAQIMCEMENGVYGAFNFHFNIPNSISN
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCEEEEEEEEEECCCCCCC
NTMEIYGTKGSIVCYDTIGREDVGKVLIKLSGENGEIDFSDYTLEELSINPVDRYEKEIQ
CEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCEEECCCCCHHHCCCCCHHHHHHHHH
AFSDAVINNEELPVSGEAGLNVVRITSAIYKSYKNKRFVNVY
HHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKLRWGIIGASGIADECTIPGILKTNNSEVVAVMSRSIEKAEKIREKYNIKKVYSNVEDL
CEEEEEEEECCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNDKEVDAVYIATPVFDHKNSVILSAKAKKHIFLEKPMGLTTEECEEMIKVTKENDVKLG
CCCCCCCEEEEEECEECCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCHH
IAFMMRFGEHHRKVRKLLKENIIGDIINTRIQYSWWYPDDNVWRLDPKLSGGGTVVDLGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCEEEEHHH
HCIDLIQYLLDTKIKTVSAMLGTKTFNYEVEDSAQIMCEMENGVYGAFNFHFNIPNSISN
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCEEEEEEEEEECCCCCCC
NTMEIYGTKGSIVCYDTIGREDVGKVLIKLSGENGEIDFSDYTLEELSINPVDRYEKEIQ
CEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCEEECCCCCHHHCCCCCHHHHHHHHH
AFSDAVINNEELPVSGEAGLNVVRITSAIYKSYKNKRFVNVY
HHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA