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The map label for this gene is rpoD [H]

Identifier: 28210583

GI number: 28210583

Start: 921750

End: 922598

Strand: Direct

Name: rpoD [H]

Synonym: CTC00867

Alternate gene names: 28210583

Gene position: 921750-922598 (Clockwise)

Preceding gene: 28210582

Following gene: 28210585

Centisome position: 32.93

GC content: 30.39

Gene sequence:

>849_bases
TTGGAGGCTAGTAACGCAAAAGTAGTGGACGATTATATAAATATGTATTTAAAAGAGATTGGTCAATCAGATTTACTTAC
AGCTGAAGAGGAAATTGATTTAGCAAAGAGAATTGAAAAGGGCGATGAAAGAGCAAAAAGTGAGTTAATTAAAAGTAATT
TAAGATTAGTTGTTAGCATAGCTAAGAAGTATATAGGAAGAGGATTAAGTTTTTTGGATTTAGTGCAAGAAGGAAACCTA
GGATTGATGAAAGCTGTAGATAAATATGATTATAAAAAAGGATATAGATTTAGTACTTATGCTACTTGGTGGATAAAACA
AGCGGTAACAAGATCAATAGCCGATCAATCAAGGACTATAAGAATACCTGTACATATGAATGAAGTGATTAGTAAGATGA
TAAGAACTAAAAAGAAATTAGAACAAGAATTAGATAGAGAACCAACAGAGGAAGAAATAGCAGAAACAGCAGAAATTTCT
ATAGAAAAAATAAAGAGAATATATAATGTTTCTCAGGATCCTGTATCTTTAGAAACACCTGTAGGTGAAAACGAAGATAC
TATATTACAAAATTTTATATCAGATGATAGATATTTACCAGAAGATAAAGTAACAGAACAAATGTTAGGAGATACATTAT
CTAATGTTTTATCTACATTATCACCCAGAGAAGAAAGAATAGTTAGATTAAGATACGGTTTAGTTGGAGATGGAGAGAGT
AGAACCTTAGAAGAGATTGGAAGAGAATTTAATTTGACAAGGGAAAGAATAAGACAAATAGAGGCAAGAGCAATAAGAAA
ACTTAGACATCCAACTAGAATTAAGAAATTACAAGGCTATTTTGAGTAA

Upstream 100 bases:

>100_bases
AGGAATAAGTAGAAGTTTATTGAAGTATATAATCTGGTGCCATATTTCAAATATGAATTACCTATAAGCATAACAGGATA
TGTAAAAGGGGTGAAAATAA

Downstream 100 bases:

>100_bases
ACCATAAGCTAGGAGATTAAAGTTTCCTAGCTTATTTTATACTTTCAAAATATCTTTTATAACTTGAGAAGCTATAATCA
TTCCAGCAACGGGAGGAACA

Product: RNA polymerase sigma factor rpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEASNAKVVDDYINMYLKEIGQSDLLTAEEEIDLAKRIEKGDERAKSELIKSNLRLVVSIAKKYIGRGLSFLDLVQEGNL
GLMKAVDKYDYKKGYRFSTYATWWIKQAVTRSIADQSRTIRIPVHMNEVISKMIRTKKKLEQELDREPTEEEIAETAEIS
IEKIKRIYNVSQDPVSLETPVGENEDTILQNFISDDRYLPEDKVTEQMLGDTLSNVLSTLSPREERIVRLRYGLVGDGES
RTLEEIGREFNLTRERIRQIEARAIRKLRHPTRIKKLQGYFE

Sequences:

>Translated_282_residues
MEASNAKVVDDYINMYLKEIGQSDLLTAEEEIDLAKRIEKGDERAKSELIKSNLRLVVSIAKKYIGRGLSFLDLVQEGNL
GLMKAVDKYDYKKGYRFSTYATWWIKQAVTRSIADQSRTIRIPVHMNEVISKMIRTKKKLEQELDREPTEEEIAETAEIS
IEKIKRIYNVSQDPVSLETPVGENEDTILQNFISDDRYLPEDKVTEQMLGDTLSNVLSTLSPREERIVRLRYGLVGDGES
RTLEEIGREFNLTRERIRQIEARAIRKLRHPTRIKKLQGYFE
>Mature_282_residues
MEASNAKVVDDYINMYLKEIGQSDLLTAEEEIDLAKRIEKGDERAKSELIKSNLRLVVSIAKKYIGRGLSFLDLVQEGNL
GLMKAVDKYDYKKGYRFSTYATWWIKQAVTRSIADQSRTIRIPVHMNEVISKMIRTKKKLEQELDREPTEEEIAETAEIS
IEKIKRIYNVSQDPVSLETPVGENEDTILQNFISDDRYLPEDKVTEQMLGDTLSNVLSTLSPREERIVRLRYGLVGDGES
RTLEEIGREFNLTRERIRQIEARAIRKLRHPTRIKKLQGYFE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=270, Percent_Identity=51.1111111111111, Blast_Score=287, Evalue=5e-79,
Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=43.1297709923664, Blast_Score=230, Evalue=8e-62,
Organism=Escherichia coli, GI1789871, Length=277, Percent_Identity=28.5198555956679, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788231, Length=210, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32718; Mature: 32718

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEASNAKVVDDYINMYLKEIGQSDLLTAEEEIDLAKRIEKGDERAKSELIKSNLRLVVSI
CCCCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKYIGRGLSFLDLVQEGNLGLMKAVDKYDYKKGYRFSTYATWWIKQAVTRSIADQSRTI
HHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEE
RIPVHMNEVISKMIRTKKKLEQELDREPTEEEIAETAEISIEKIKRIYNVSQDPVSLETP
EECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VGENEDTILQNFISDDRYLPEDKVTEQMLGDTLSNVLSTLSPREERIVRLRYGLVGDGES
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCH
RTLEEIGREFNLTRERIRQIEARAIRKLRHPTRIKKLQGYFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCC
>Mature Secondary Structure
MEASNAKVVDDYINMYLKEIGQSDLLTAEEEIDLAKRIEKGDERAKSELIKSNLRLVVSI
CCCCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKKYIGRGLSFLDLVQEGNLGLMKAVDKYDYKKGYRFSTYATWWIKQAVTRSIADQSRTI
HHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEE
RIPVHMNEVISKMIRTKKKLEQELDREPTEEEIAETAEISIEKIKRIYNVSQDPVSLETP
EECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VGENEDTILQNFISDDRYLPEDKVTEQMLGDTLSNVLSTLSPREERIVRLRYGLVGDGES
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCH
RTLEEIGREFNLTRERIRQIEARAIRKLRHPTRIKKLQGYFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]