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The map label for this gene is trxB [C]

Identifier: 28210559

GI number: 28210559

Start: 893891

End: 894838

Strand: Direct

Name: trxB [C]

Synonym: CTC00842

Alternate gene names: 28210559

Gene position: 893891-894838 (Clockwise)

Preceding gene: 28210557

Following gene: 28210560

Centisome position: 31.93

GC content: 29.96

Gene sequence:

>948_bases
GTGAATAAAGAAGAAAAGATACTGGATTTAGTTATTATAGGCTCTGGACCAGCGGGATTAACGGCAGCTATATATGCATC
TAGAATGAAATTAGATTTTATAGTATTAGAAAATGAGCTTGTTGGAGGTCAGATAAGATCTACCTATTCTATAGAGAATT
ATCCAGGTTTTAGAAGTATATCCGGTGAAGAATTAGTAAATAAGATATATGATCATGCAGTGGATTTAGGAGCAAAGATA
GATGAATTTGACGAAATACAATATATAAAATTAACAGAGGAAGAAAAGCTAATAGAAACATATGAATATATATATAAACC
TAAAGCAGTAATAATAGCAGCAGGTGCTAAATACAGACCGCTTCCTATTCCAGAAGAGAAAAAGTACCATGGTAATGGAA
TTCACCATTGTGAGTTGTGTGATGGAGAATTTTATCAGAATAAGAGTATAATGGTGGTAGGAGGAGGAAATTCAGCTTTA
GAAGGAGCTATATTTTTATCAAGATATGCAAAGAATATAACTATGGTGCATCAACTTGATTATTTTCAAGGAGAGAAATC
CCTTCAAGATGAGGTGTTTAATAATAGTAAGATAAAAATACTTTGGAATACAGATATTAAAAATGCTTTTGGTGAAAATA
AATTAGAAGAGGTTTTAGTAGAAGATTTAAAGACAAAAGAACAATATAAAATAAATGTTGATGGGATATTTGTTTATATA
GGTATGGTTCCTAATACAGATTTATATAAAGATTATATAAATTTAAATGAAAGAGGATATATAATAGCTGATGAAAGTAC
AAAAACAAATATAGAAGGAGTTTTTGCAGCAGGTGATGTAAGAGCAAAACAATTTAGACAATTAGTAACAGCTAATGCAG
ATGGAGCTGTAGCAGCATTATCTGCTGAAAAATATATATCTAAAAAGAGGAGGGAAAATAATGATTAA

Upstream 100 bases:

>100_bases
ATTTATAATGTGAAGTATTGACAGAAATAAAGATGTATATTACTATATTAGTTAATAATTACTATTAATTAACATAAAAT
ATTGATTGGCAGGTGATTAG

Downstream 100 bases:

>100_bases
AGTATATTCAACACCAACTTGCCCTTATTGTAAGAAAGCAAAAGCTTATTTTGATTCAAAAAATGTAGAGTATAAAGATG
TTAATGTAGCACAAGATGAA

Product: thioredoxin reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNKEEKILDLVIIGSGPAGLTAAIYASRMKLDFIVLENELVGGQIRSTYSIENYPGFRSISGEELVNKIYDHAVDLGAKI
DEFDEIQYIKLTEEEKLIETYEYIYKPKAVIIAAGAKYRPLPIPEEKKYHGNGIHHCELCDGEFYQNKSIMVVGGGNSAL
EGAIFLSRYAKNITMVHQLDYFQGEKSLQDEVFNNSKIKILWNTDIKNAFGENKLEEVLVEDLKTKEQYKINVDGIFVYI
GMVPNTDLYKDYINLNERGYIIADESTKTNIEGVFAAGDVRAKQFRQLVTANADGAVAALSAEKYISKKRRENND

Sequences:

>Translated_315_residues
MNKEEKILDLVIIGSGPAGLTAAIYASRMKLDFIVLENELVGGQIRSTYSIENYPGFRSISGEELVNKIYDHAVDLGAKI
DEFDEIQYIKLTEEEKLIETYEYIYKPKAVIIAAGAKYRPLPIPEEKKYHGNGIHHCELCDGEFYQNKSIMVVGGGNSAL
EGAIFLSRYAKNITMVHQLDYFQGEKSLQDEVFNNSKIKILWNTDIKNAFGENKLEEVLVEDLKTKEQYKINVDGIFVYI
GMVPNTDLYKDYINLNERGYIIADESTKTNIEGVFAAGDVRAKQFRQLVTANADGAVAALSAEKYISKKRRENND
>Mature_315_residues
MNKEEKILDLVIIGSGPAGLTAAIYASRMKLDFIVLENELVGGQIRSTYSIENYPGFRSISGEELVNKIYDHAVDLGAKI
DEFDEIQYIKLTEEEKLIETYEYIYKPKAVIIAAGAKYRPLPIPEEKKYHGNGIHHCELCDGEFYQNKSIMVVGGGNSAL
EGAIFLSRYAKNITMVHQLDYFQGEKSLQDEVFNNSKIKILWNTDIKNAFGENKLEEVLVEDLKTKEQYKINVDGIFVYI
GMVPNTDLYKDYINLNERGYIIADESTKTNIEGVFAAGDVRAKQFRQLVTANADGAVAALSAEKYISKKRRENND

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=309, Percent_Identity=36.5695792880259, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI87081763, Length=315, Percent_Identity=33.015873015873, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=34.9358974358974, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321898, Length=314, Percent_Identity=35.031847133758, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 35553; Mature: 35553

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEEKILDLVIIGSGPAGLTAAIYASRMKLDFIVLENELVGGQIRSTYSIENYPGFRSI
CCCCCEEEEEEEECCCCCCHHHHHHHHHEEEEEEEEECCCCCCEEEEEEEECCCCCCCCC
SGEELVNKIYDHAVDLGAKIDEFDEIQYIKLTEEEKLIETYEYIYKPKAVIIAAGAKYRP
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCC
LPIPEEKKYHGNGIHHCELCDGEFYQNKSIMVVGGGNSALEGAIFLSRYAKNITMVHQLD
CCCCCCCCCCCCCCEEEEECCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEH
YFQGEKSLQDEVFNNSKIKILWNTDIKNAFGENKLEEVLVEDLKTKEQYKINVDGIFVYI
HHCCCHHHHHHHCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHCCCCEEEEEECEEEEEE
GMVPNTDLYKDYINLNERGYIIADESTKTNIEGVFAAGDVRAKQFRQLVTANADGAVAAL
EECCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEE
SAEKYISKKRRENND
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKEEKILDLVIIGSGPAGLTAAIYASRMKLDFIVLENELVGGQIRSTYSIENYPGFRSI
CCCCCEEEEEEEECCCCCCHHHHHHHHHEEEEEEEEECCCCCCEEEEEEEECCCCCCCCC
SGEELVNKIYDHAVDLGAKIDEFDEIQYIKLTEEEKLIETYEYIYKPKAVIIAAGAKYRP
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCC
LPIPEEKKYHGNGIHHCELCDGEFYQNKSIMVVGGGNSALEGAIFLSRYAKNITMVHQLD
CCCCCCCCCCCCCCEEEEECCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEH
YFQGEKSLQDEVFNNSKIKILWNTDIKNAFGENKLEEVLVEDLKTKEQYKINVDGIFVYI
HHCCCHHHHHHHCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHCCCCEEEEEECEEEEEE
GMVPNTDLYKDYINLNERGYIIADESTKTNIEGVFAAGDVRAKQFRQLVTANADGAVAAL
EECCCCHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEE
SAEKYISKKRRENND
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]