Click here to switch to the map view.

The map label for this gene is braB [H]

Identifier: 28210504

GI number: 28210504

Start: 833605

End: 834909

Strand: Direct

Name: braB [H]

Synonym: CTC00787

Alternate gene names: 28210504

Gene position: 833605-834909 (Clockwise)

Preceding gene: 28210503

Following gene: 28210507

Centisome position: 29.78

GC content: 28.74

Gene sequence:

>1305_bases
TTGAAGAAGAATTTAAAAGAAGTATTAATAACAGGATTTGCTTTATTCTCTATGTTTTTTGGAGCAGGAAATTTAATATT
TCCACCAACACTTGGATATATGGCGGGGAAGAACTGGGGCATCGCAACAATTGGATTTTTAATAACTTGTATAGGTCTTC
CTATTTTAGGTATTATATCCATAGCCATGGTAGGTGGAACTATTGAGAAGTTTACAAATAAAGTTGGAAAAAATTTTGGG
AAGATACTTTTTACAGTAATAATGTTAGCAATAGGTCCATTATTTTGTATACCCAGAACTGGAGCAACAACTTTTGAATT
AGGTGTTTTACCTATGTTTCCTAATTCAAATCCAATACTTTTTTCTATAATATTTTTTGGTATAAGTTATATATTTTCAT
CAAATGAATCAAAAGTAGTAGATAAAATAGGTATTATACTTACACCTATTTTATTAGGAAGTTTAGGACTAATAATATTA
AAAGGAATTATTAATCCTATAGGAGTACCTACAGTATCAGATTTACAAAATCCTTTTTCAACAGGATTTACAGAAGGATA
TCAGACTATGGATGCAATTGGGTCTATGATTGTGGCTCAAATGGTTATAGTAAATTTAATAGTTAAAGGTTATAAAAGTA
AGGAAGAACAGGTGAATATAACATCTAAATCAGGAATAGTAGCAGCAATATGTTTAGTATTAGTTTATGGAGGACTTTCA
TATATAGGATCCACTGCAAAGTCAGTTTTTCCAACTAATATGTCAAGAACAACTTTACTTATAGAAATAACCCAAAGTAT
ATTAGGTCCTTATAGTAAGTATATATTTGGAGGAGCTATATCAATTGCTTGTCTTACAACCTCAGTGGGTTTAACAGCTA
CATGTGGAAACTATTTTAATAAACTATCAAATAATAAAATAAGCTATAAAACTGTTGTTTTAATTATAAGTATATTCAGT
TGTGTAATATCTAATTATGGAGTTGAAACTATAATTAATTTAGCAGTACCAGTATTAGTTACACTTTATCCTGTGGTTAT
TATATTAATCTTATTAAACTTATTCAACAAGATTATACCTAATAGAGGAACTTATATAGGATCAGTATATGGGGCGTTAA
TTGTAAGTGTATTCATGTCACTAGAATCTGTCGGGGTAAAAATACCTTTTATAGTAGATGTAATATATCAATTTCCTTTA
GCTAAAGAAGGGTTCTTCTGGATAGTTCCATCTTTATTGGGAGGTATTTGTGGAACAATACTAGATTATAGAAGAAAAAA
TAATTTTTCATATAAAGATGCATAA

Upstream 100 bases:

>100_bases
GGTTATGCAAATCAACTAGGGTGGTAACACGGAGCATCGTCCCTTTAGGGATGATGCTCTTTTTTAATTCAAATATAAAA
ATAAAAAAGGGGGATTTATT

Downstream 100 bases:

>100_bases
AATAAAAAGTCTATCTTTTTTATTATAATAAAATGTACCCTTTGTCAAGGACAGTTTAAAAAAAGACTAGGCGGTACTAA
GAAGATGATTTCTGTATTTT

Product: branched-chain amino acid transport system carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKKNLKEVLITGFALFSMFFGAGNLIFPPTLGYMAGKNWGIATIGFLITCIGLPILGIISIAMVGGTIEKFTNKVGKNFG
KILFTVIMLAIGPLFCIPRTGATTFELGVLPMFPNSNPILFSIIFFGISYIFSSNESKVVDKIGIILTPILLGSLGLIIL
KGIINPIGVPTVSDLQNPFSTGFTEGYQTMDAIGSMIVAQMVIVNLIVKGYKSKEEQVNITSKSGIVAAICLVLVYGGLS
YIGSTAKSVFPTNMSRTTLLIEITQSILGPYSKYIFGGAISIACLTTSVGLTATCGNYFNKLSNNKISYKTVVLIISIFS
CVISNYGVETIINLAVPVLVTLYPVVIILILLNLFNKIIPNRGTYIGSVYGALIVSVFMSLESVGVKIPFIVDVIYQFPL
AKEGFFWIVPSLLGGICGTILDYRRKNNFSYKDA

Sequences:

>Translated_434_residues
MKKNLKEVLITGFALFSMFFGAGNLIFPPTLGYMAGKNWGIATIGFLITCIGLPILGIISIAMVGGTIEKFTNKVGKNFG
KILFTVIMLAIGPLFCIPRTGATTFELGVLPMFPNSNPILFSIIFFGISYIFSSNESKVVDKIGIILTPILLGSLGLIIL
KGIINPIGVPTVSDLQNPFSTGFTEGYQTMDAIGSMIVAQMVIVNLIVKGYKSKEEQVNITSKSGIVAAICLVLVYGGLS
YIGSTAKSVFPTNMSRTTLLIEITQSILGPYSKYIFGGAISIACLTTSVGLTATCGNYFNKLSNNKISYKTVVLIISIFS
CVISNYGVETIINLAVPVLVTLYPVVIILILLNLFNKIIPNRGTYIGSVYGALIVSVFMSLESVGVKIPFIVDVIYQFPL
AKEGFFWIVPSLLGGICGTILDYRRKNNFSYKDA
>Mature_434_residues
MKKNLKEVLITGFALFSMFFGAGNLIFPPTLGYMAGKNWGIATIGFLITCIGLPILGIISIAMVGGTIEKFTNKVGKNFG
KILFTVIMLAIGPLFCIPRTGATTFELGVLPMFPNSNPILFSIIFFGISYIFSSNESKVVDKIGIILTPILLGSLGLIIL
KGIINPIGVPTVSDLQNPFSTGFTEGYQTMDAIGSMIVAQMVIVNLIVKGYKSKEEQVNITSKSGIVAAICLVLVYGGLS
YIGSTAKSVFPTNMSRTTLLIEITQSILGPYSKYIFGGAISIACLTTSVGLTATCGNYFNKLSNNKISYKTVVLIISIFS
CVISNYGVETIINLAVPVLVTLYPVVIILILLNLFNKIIPNRGTYIGSVYGALIVSVFMSLESVGVKIPFIVDVIYQFPL
AKEGFFWIVPSLLGGICGTILDYRRKNNFSYKDA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=426, Percent_Identity=32.6291079812207, Blast_Score=225, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46789; Mature: 46789

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]