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The map label for this gene is hemB [H]

Identifier: 28210450

GI number: 28210450

Start: 755228

End: 756217

Strand: Direct

Name: hemB [H]

Synonym: CTC00729

Alternate gene names: 28210450

Gene position: 755228-756217 (Clockwise)

Preceding gene: 28210449

Following gene: 28210451

Centisome position: 26.98

GC content: 32.32

Gene sequence:

>990_bases
GTGAAAATTATGAATTTTAGAAGACAAAGAAGACTTAGAAAAAATGAAGCTATAAGAAGTATGGTAAGGGAAACTATATT
AACTACAAATGACTTTATATATCCTATATTTGTAACAGAAGGAAATAATATAAAAAATGAAATAAGTTCTCTACCAGGTA
ATTATCACTATTCTATAGATAGATTAAATGAAGTTATTGAAGAGGTAATAAAAGCTAAAATAAAAGGAGTAATACTATTT
GGAATACCAAATGAAAAAGATGAAATAGGTAGTGAAGGTTTTAGTGATGATGGTATTATTCAAAAAGCTATAAGAAAAAT
AAAAGAACTATCCCCAGAACTTTATGTAATTACAGATGTATGTATGTGTGAGTATACAAGCCATGGACATTGTGGAATAA
TTCATGGAAATGATGTAGATAATGATGAAACATTAGAATACTTAGGCAGGATTGCTCTATCACATGTAAAGGCAGGGGCA
GATATGGTAGCTCCTTCTGATATGATGGATGGAAGAATTGCATTTATGAGGGATGTATTGGATAAAAGTGGATATAAGGA
CATACCAATAATGAGTTATAGTGCAAAATATTGCTCAGCTTTTTATGGGCCATTTAGGGAAGCAGCAGATTCAGCACCAC
AATTTGGAGATAGAAAAACCTATCAAATGGATCCTGCTAACGTAAGAGAAGCCATGATAGAGGTTGAAAATGACCTAGAA
GAAGGAGCGGACATTATAATGGTTAAACCAGCTCTTCCTTATTTAGATGTTATAAGATGGGCAAGGGATAAAACTAATGT
ACCAATAGCTGCTTATAATGTAAGTGGAGAATATGCAATGGTTAAAGCAGCAGCGAAAATGGGATGGATAGATGAAAAAA
GAGTAGCTTTAGAAATGCTTCTTTCTATAAAAAGAGCGGGAGCAGATATGATAATAACTTATTATGCTCTACAAGCATCA
AAATGGTTAGGAGAGGATAAATGTGAATAA

Upstream 100 bases:

>100_bases
ACAGCCAAGGAACTAGATAAAAATGGTATAGATTATATTATCTGTGATGAATATACTACAGATGGAATGATAGAAAAACT
TTTAACTTTATAAAGAGGAT

Downstream 100 bases:

>100_bases
TTTAGAAATATTTAAGGAATCTGAAAAATATATGCCAGGTGGAGTTAATAGTCCTGTAAGAGCTTTTAGCAATGCATCTA
TAAATCCACCAATAATAAAA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIMNFRRQRRLRKNEAIRSMVRETILTTNDFIYPIFVTEGNNIKNEISSLPGNYHYSIDRLNEVIEEVIKAKIKGVILF
GIPNEKDEIGSEGFSDDGIIQKAIRKIKELSPELYVITDVCMCEYTSHGHCGIIHGNDVDNDETLEYLGRIALSHVKAGA
DMVAPSDMMDGRIAFMRDVLDKSGYKDIPIMSYSAKYCSAFYGPFREAADSAPQFGDRKTYQMDPANVREAMIEVENDLE
EGADIIMVKPALPYLDVIRWARDKTNVPIAAYNVSGEYAMVKAAAKMGWIDEKRVALEMLLSIKRAGADMIITYYALQAS
KWLGEDKCE

Sequences:

>Translated_329_residues
MKIMNFRRQRRLRKNEAIRSMVRETILTTNDFIYPIFVTEGNNIKNEISSLPGNYHYSIDRLNEVIEEVIKAKIKGVILF
GIPNEKDEIGSEGFSDDGIIQKAIRKIKELSPELYVITDVCMCEYTSHGHCGIIHGNDVDNDETLEYLGRIALSHVKAGA
DMVAPSDMMDGRIAFMRDVLDKSGYKDIPIMSYSAKYCSAFYGPFREAADSAPQFGDRKTYQMDPANVREAMIEVENDLE
EGADIIMVKPALPYLDVIRWARDKTNVPIAAYNVSGEYAMVKAAAKMGWIDEKRVALEMLLSIKRAGADMIITYYALQAS
KWLGEDKCE
>Mature_329_residues
MKIMNFRRQRRLRKNEAIRSMVRETILTTNDFIYPIFVTEGNNIKNEISSLPGNYHYSIDRLNEVIEEVIKAKIKGVILF
GIPNEKDEIGSEGFSDDGIIQKAIRKIKELSPELYVITDVCMCEYTSHGHCGIIHGNDVDNDETLEYLGRIALSHVKAGA
DMVAPSDMMDGRIAFMRDVLDKSGYKDIPIMSYSAKYCSAFYGPFREAADSAPQFGDRKTYQMDPANVREAMIEVENDLE
EGADIIMVKPALPYLDVIRWARDKTNVPIAAYNVSGEYAMVKAAAKMGWIDEKRVALEMLLSIKRAGADMIITYYALQAS
KWLGEDKCE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=307, Percent_Identity=44.299674267101, Blast_Score=278, Evalue=6e-75,
Organism=Escherichia coli, GI87081728, Length=311, Percent_Identity=51.1254019292604, Blast_Score=313, Evalue=7e-87,
Organism=Saccharomyces cerevisiae, GI6321398, Length=315, Percent_Identity=42.8571428571429, Blast_Score=249, Evalue=3e-67,
Organism=Drosophila melanogaster, GI21358291, Length=292, Percent_Identity=40.7534246575342, Blast_Score=250, Evalue=7e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37199; Mature: 37199

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIMNFRRQRRLRKNEAIRSMVRETILTTNDFIYPIFVTEGNNIKNEISSLPGNYHYSID
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCCCCEEEHH
RLNEVIEEVIKAKIKGVILFGIPNEKDEIGSEGFSDDGIIQKAIRKIKELSPELYVITDV
HHHHHHHHHHHHHHCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHH
CMCEYTSHGHCGIIHGNDVDNDETLEYLGRIALSHVKAGADMVAPSDMMDGRIAFMRDVL
HHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHH
DKSGYKDIPIMSYSAKYCSAFYGPFREAADSAPQFGDRKTYQMDPANVREAMIEVENDLE
HHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
EGADIIMVKPALPYLDVIRWARDKTNVPIAAYNVSGEYAMVKAAAKMGWIDEKRVALEML
CCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHH
LSIKRAGADMIITYYALQASKWLGEDKCE
HHHHHCCCCEEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIMNFRRQRRLRKNEAIRSMVRETILTTNDFIYPIFVTEGNNIKNEISSLPGNYHYSID
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCCCCEEEHH
RLNEVIEEVIKAKIKGVILFGIPNEKDEIGSEGFSDDGIIQKAIRKIKELSPELYVITDV
HHHHHHHHHHHHHHCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHH
CMCEYTSHGHCGIIHGNDVDNDETLEYLGRIALSHVKAGADMVAPSDMMDGRIAFMRDVL
HHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHH
DKSGYKDIPIMSYSAKYCSAFYGPFREAADSAPQFGDRKTYQMDPANVREAMIEVENDLE
HHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHH
EGADIIMVKPALPYLDVIRWARDKTNVPIAAYNVSGEYAMVKAAAKMGWIDEKRVALEML
CCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHH
LSIKRAGADMIITYYALQASKWLGEDKCE
HHHHHCCCCEEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]