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The map label for this gene is pleD [H]

Identifier: 28210427

GI number: 28210427

Start: 727518

End: 728144

Strand: Direct

Name: pleD [H]

Synonym: CTC00705

Alternate gene names: 28210427

Gene position: 727518-728144 (Clockwise)

Preceding gene: 28210426

Following gene: 28210428

Centisome position: 25.99

GC content: 25.36

Gene sequence:

>627_bases
ATGGTTGACAATGAAAAGGTGATTGAATGGAGTTATTATACTAGCTGGTCAAGAGAACAGCTAGTTGAAAGGATTAAAGA
GTTAGAAAAACTTACACAAAATCTAAAGGAGACGGAAATAAGTTTAATTGAAAAAAATAAGTTTTTAAAAGAAATAAGTA
TGGAGGATTGTCTTACAAAGGTTTATAATTATAGAACTATAATAGAAATAATAGAAAGGCAAATTAAATATTCTAATTGC
AAGGGGGAAACATTTTGTATATTAATGATAGATATAGATGATTTTAAAAGTGTTAATGATAATTTTGGACATGTTTTTGG
AGATGAAATACTTATAAAAATATCTAAAGAAATTAAAAAAAATATAAGGAAAAATGATTATGTTGGTAGATTTGGAGGAG
AAGAGTTTATAATAATATTTAATAATACCAAATTATCTGAGGCTATTAGAGTATCTGAAAGAATAAGAAAATCCATAGAG
GGGTTAGAATTTTTTAGATCATTAAAAATAACTATAAGTGGTGGAGTAAAAGAATACTCTAATGAAAGTTGCAAAGAAAT
TATAGATGCTGCAGACAATTTTCTTTATAAGGCCAAAGGATTAGGAAAAAATAGAATATGTTATTAA

Upstream 100 bases:

>100_bases
GAAATTAAAAACATAATTAAGGATATGTTAAATATATATATTTAATTATACCTTAGGTGTTTTTATAAGGTTAAAAAACA
TTTAAAAAGGGTGGGGAATT

Downstream 100 bases:

>100_bases
TTATAAAATCGCCATATTAAAAGGAATAAATCCTGATATGGCGATTTTTAAGCTTAGATTTATTTTTGTTAATGATTAAT
TGGCATAATACTTGCGTTTG

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MVDNEKVIEWSYYTSWSREQLVERIKELEKLTQNLKETEISLIEKNKFLKEISMEDCLTKVYNYRTIIEIIERQIKYSNC
KGETFCILMIDIDDFKSVNDNFGHVFGDEILIKISKEIKKNIRKNDYVGRFGGEEFIIIFNNTKLSEAIRVSERIRKSIE
GLEFFRSLKITISGGVKEYSNESCKEIIDAADNFLYKAKGLGKNRICY

Sequences:

>Translated_208_residues
MVDNEKVIEWSYYTSWSREQLVERIKELEKLTQNLKETEISLIEKNKFLKEISMEDCLTKVYNYRTIIEIIERQIKYSNC
KGETFCILMIDIDDFKSVNDNFGHVFGDEILIKISKEIKKNIRKNDYVGRFGGEEFIIIFNNTKLSEAIRVSERIRKSIE
GLEFFRSLKITISGGVKEYSNESCKEIIDAADNFLYKAKGLGKNRICY
>Mature_208_residues
MVDNEKVIEWSYYTSWSREQLVERIKELEKLTQNLKETEISLIEKNKFLKEISMEDCLTKVYNYRTIIEIIERQIKYSNC
KGETFCILMIDIDDFKSVNDNFGHVFGDEILIKISKEIKKNIRKNDYVGRFGGEEFIIIFNNTKLSEAIRVSERIRKSIE
GLEFFRSLKITISGGVKEYSNESCKEIIDAADNFLYKAKGLGKNRICY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=38.2716049382716, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=33.7142857142857, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=33.9622641509434, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=33.9393939393939, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=34.1463414634146, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=31.8471337579618, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787802, Length=212, Percent_Identity=26.8867924528302, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=29.8780487804878, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=143, Percent_Identity=29.3706293706294, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=27.7173913043478, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=173, Percent_Identity=25.4335260115607, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=28.4848484848485, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=131, Percent_Identity=35.8778625954198, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=201, Percent_Identity=26.865671641791, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 24464; Mature: 24464

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDNEKVIEWSYYTSWSREQLVERIKELEKLTQNLKETEISLIEKNKFLKEISMEDCLTK
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYNYRTIIEIIERQIKYSNCKGETFCILMIDIDDFKSVNDNFGHVFGDEILIKISKEIKK
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NIRKNDYVGRFGGEEFIIIFNNTKLSEAIRVSERIRKSIEGLEFFRSLKITISGGVKEYS
HHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHC
NESCKEIIDAADNFLYKAKGLGKNRICY
CHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVDNEKVIEWSYYTSWSREQLVERIKELEKLTQNLKETEISLIEKNKFLKEISMEDCLTK
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYNYRTIIEIIERQIKYSNCKGETFCILMIDIDDFKSVNDNFGHVFGDEILIKISKEIKK
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
NIRKNDYVGRFGGEEFIIIFNNTKLSEAIRVSERIRKSIEGLEFFRSLKITISGGVKEYS
HHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHC
NESCKEIIDAADNFLYKAKGLGKNRICY
CHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA