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The map label for this gene is ribH

Identifier: 28210398

GI number: 28210398

Start: 691944

End: 692405

Strand: Direct

Name: ribH

Synonym: CTC00674

Alternate gene names: 28210398

Gene position: 691944-692405 (Clockwise)

Preceding gene: 28210397

Following gene: 28210399

Centisome position: 24.72

GC content: 32.25

Gene sequence:

>462_bases
ATGAATATAATAGAAGGAAAATTAATAGGACAGAGTTTGAAGTTTGGTATAACTATAGGAAGATTTAATGAATTTATAGG
AGGTAAACTTTTAGATGGAGCTGTGGATGCACTTATAAGACATGGTGTAGATGAAAAAGACATAGAAATAGCTTGGGTGC
CAGGAGCTTTTGAAATACCATTAATAGCTAAAAAGATGGCAAAAAGTAAAAAATATGATGGGATAATATGCTTAGGTGCT
GTTATAAGAGGAGCCACTACTCATTATGACTATGTGGCAAGTGAAGTATCTAAGGGTATAGCTAAAATTACTTTAGATGA
AGAAGTGCCAGTAATATTTGGAGTTTTAACTACAGAAAATATAGAACAAGCCATAGAAAGGGCAGGAACAAAAGCTGGTA
ATAAAGGATATGAGGCAGCTTGTACTGCAATAGAAATGGCAAATATTATAAATATAATATAA

Upstream 100 bases:

>100_bases
ATTCATGAAAATGAGGAAAGTGAATTTTACCTAAAAACTAAAAAAGAAAAAATGGGTCATTTATTACATTTTTAATATAT
AAAATTGGGAGGAATATAAA

Downstream 100 bases:

>100_bases
ATCAAAAAATTATAAAAATATAAAATTTGAACAAACCTATTCTTTTTTTATACAATGCATAACAAATCTTAAAATCAGGT
TGACAAGCAAATTGCTAATA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNIIEGKLIGQSLKFGITIGRFNEFIGGKLLDGAVDALIRHGVDEKDIEIAWVPGAFEIPLIAKKMAKSKKYDGIICLGA
VIRGATTHYDYVASEVSKGIAKITLDEEVPVIFGVLTTENIEQAIERAGTKAGNKGYEAACTAIEMANIINII

Sequences:

>Translated_153_residues
MNIIEGKLIGQSLKFGITIGRFNEFIGGKLLDGAVDALIRHGVDEKDIEIAWVPGAFEIPLIAKKMAKSKKYDGIICLGA
VIRGATTHYDYVASEVSKGIAKITLDEEVPVIFGVLTTENIEQAIERAGTKAGNKGYEAACTAIEMANIINII
>Mature_153_residues
MNIIEGKLIGQSLKFGITIGRFNEFIGGKLLDGAVDALIRHGVDEKDIEIAWVPGAFEIPLIAKKMAKSKKYDGIICLGA
VIRGATTHYDYVASEVSKGIAKITLDEEVPVIFGVLTTENIEQAIERAGTKAGNKGYEAACTAIEMANIINII

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=57.7922077922078, Blast_Score=179, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324429, Length=147, Percent_Identity=32.6530612244898, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CLOTE (Q897Q7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781342.1
- ProteinModelPortal:   Q897Q7
- SMR:   Q897Q7
- GeneID:   1060294
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00674
- NMPDR:   fig|212717.1.peg.564
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q897Q7
- ProtClustDB:   PRK00061
- BioCyc:   CTET212717:CTC_00674-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16436; Mature: 16436

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIEGKLIGQSLKFGITIGRFNEFIGGKLLDGAVDALIRHGVDEKDIEIAWVPGAFEIP
CCCCCCHHHCCHHHHEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
LIAKKMAKSKKYDGIICLGAVIRGATTHYDYVASEVSKGIAKITLDEEVPVIFGVLTTEN
HHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECHHH
IEQAIERAGTKAGNKGYEAACTAIEMANIINII
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIIEGKLIGQSLKFGITIGRFNEFIGGKLLDGAVDALIRHGVDEKDIEIAWVPGAFEIP
CCCCCCHHHCCHHHHEEEHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
LIAKKMAKSKKYDGIICLGAVIRGATTHYDYVASEVSKGIAKITLDEEVPVIFGVLTTEN
HHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECHHH
IEQAIERAGTKAGNKGYEAACTAIEMANIINII
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129