Click here to switch to the map view.

The map label for this gene is yclK [H]

Identifier: 28210333

GI number: 28210333

Start: 622296

End: 623777

Strand: Direct

Name: yclK [H]

Synonym: CTC00598

Alternate gene names: 28210333

Gene position: 622296-623777 (Clockwise)

Preceding gene: 28210332

Following gene: 28210334

Centisome position: 22.23

GC content: 26.18

Gene sequence:

>1482_bases
ATGGGGAGTAGGATACAAATTTATAGGAGAAGTGTTACCATGAAAATTAAAAATTGGATTACAGTTTCCTATATAACAGT
AATGCTCATACCTATTTTAACTGCTGTAATGTTGTTTTCTTGGATAAAAAATTATAATAGAAAAACAGAAATGGAAGATT
ATATAAAGAATATGCAAACCTTTGAAAAATATGATGAACTATTAAATAATGAAAGGTTATTTAGTTACGCTACTAAAAAA
TTAAATATAATAGATGAAAAAGACAAAGATAGTATGAGAATTGAGCTTTACGATAAAAATGGAGGAAAGATATATTCCTC
TAGTAGTGATGAATACTTTTATCCACTACCTAAAAAACAACTTTATTCAAATCTTTATGAAATAATACATGGTTTTAGAG
CAGATACACTAAAAAAACCTGTATTTAAAGATGAGGACTTAATTGGATTTTATGAGATTACCATAATGCGTAATAACATA
ATTAAAGGAGTAAATGACGCAACTATTAAAGCAATTATTATATTTATTTTAATTTTTCTAACTGTACTATTATGTATGAT
AAAGTTTATGAATAAAAAATTTAATAACCCCATTCAAATGCTCATTAAAGCCATGAAAGCTTTTCCACTAGGAGAAGAAA
GTTCTATAGATTATAAAGCTACTGATGAAATAGGAGAACTTATAAATCATTTTAATTATATGAAGGATGAAATAATAGAG
AAAAATAATGAAATAGAAAAACAACAAAAGTCCAAAGAATACATGATAGCTGCTATATCCCATGATTTAAAAACACCTTT
AACTTCTATAAGGGCCTATGCAGAACTTATGAATGTAGAAGGAGAAAAAAATAATAAATATGTAAAAGGCATATTAAATA
AATGTGACTATATGACCAATATGCTTCAAGACTTATTAATGTACACAATACTTTCATCAAACTATAATATGGAATTTGTA
GATGTAGAAGGTGAAGAATTCTTTGAAATGTTATTTTCAGGATATAAAGAACTTTGTAAAAAGAATCATATAAATTATTT
ATCAGAAATTAAGGTTAATAGAAATTATAAAGTAGATGTAAAACAAATGATAAGACTTGTGGACAACTTAATAGCCAATG
CCATAATACATACAGAAAAAGAAAAGTACATTTGCATGGGGGCAATTAGTAGCCAATATGAATTACCAAATTGGGTACAA
GGAGATGATAGGGAAAAAATTAATAATTTTAGAAAAGATCATACAGTTATAATTGTAAAAAATCAAGGAGATGAAATATC
TAAAGAAAATATAAAAAGAATTATAGAACCTTTTTATAAAGGGGACAACTCTAGAAATAAACGAGGGGGGACGGGACTAG
GATTAAGTATAGTGAAATTGATAATAGAAAAACATGGTGGAGAAATAAATATTTTATCAAGTAAAAAGAGAGGAACCTTG
ATAGTTTGTGTTTTAAAAGAGGAAAGGAATGAATTTTTATGA

Upstream 100 bases:

>100_bases
AAAAGGATGTACATGGAAATAATACAGTTACTGTGCACATAAAAGAACTTAGAGAAAAGCTAAAAGAAAATATTAAAAAT
CCTAACTTTATAGAAACAGT

Downstream 100 bases:

>100_bases
TAAAAAAGAAAATTATGAGTATATTTATGGTGGGTTTAATTGGAGTAACTGCATTAGCAGGATGTGAAGGTAAAAATAGT
ATTTTACCAGAAGAAATAGT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MGSRIQIYRRSVTMKIKNWITVSYITVMLIPILTAVMLFSWIKNYNRKTEMEDYIKNMQTFEKYDELLNNERLFSYATKK
LNIIDEKDKDSMRIELYDKNGGKIYSSSSDEYFYPLPKKQLYSNLYEIIHGFRADTLKKPVFKDEDLIGFYEITIMRNNI
IKGVNDATIKAIIIFILIFLTVLLCMIKFMNKKFNNPIQMLIKAMKAFPLGEESSIDYKATDEIGELINHFNYMKDEIIE
KNNEIEKQQKSKEYMIAAISHDLKTPLTSIRAYAELMNVEGEKNNKYVKGILNKCDYMTNMLQDLLMYTILSSNYNMEFV
DVEGEEFFEMLFSGYKELCKKNHINYLSEIKVNRNYKVDVKQMIRLVDNLIANAIIHTEKEKYICMGAISSQYELPNWVQ
GDDREKINNFRKDHTVIIVKNQGDEISKENIKRIIEPFYKGDNSRNKRGGTGLGLSIVKLIIEKHGGEINILSSKKRGTL
IVCVLKEERNEFL

Sequences:

>Translated_493_residues
MGSRIQIYRRSVTMKIKNWITVSYITVMLIPILTAVMLFSWIKNYNRKTEMEDYIKNMQTFEKYDELLNNERLFSYATKK
LNIIDEKDKDSMRIELYDKNGGKIYSSSSDEYFYPLPKKQLYSNLYEIIHGFRADTLKKPVFKDEDLIGFYEITIMRNNI
IKGVNDATIKAIIIFILIFLTVLLCMIKFMNKKFNNPIQMLIKAMKAFPLGEESSIDYKATDEIGELINHFNYMKDEIIE
KNNEIEKQQKSKEYMIAAISHDLKTPLTSIRAYAELMNVEGEKNNKYVKGILNKCDYMTNMLQDLLMYTILSSNYNMEFV
DVEGEEFFEMLFSGYKELCKKNHINYLSEIKVNRNYKVDVKQMIRLVDNLIANAIIHTEKEKYICMGAISSQYELPNWVQ
GDDREKINNFRKDHTVIIVKNQGDEISKENIKRIIEPFYKGDNSRNKRGGTGLGLSIVKLIIEKHGGEINILSSKKRGTL
IVCVLKEERNEFL
>Mature_492_residues
GSRIQIYRRSVTMKIKNWITVSYITVMLIPILTAVMLFSWIKNYNRKTEMEDYIKNMQTFEKYDELLNNERLFSYATKKL
NIIDEKDKDSMRIELYDKNGGKIYSSSSDEYFYPLPKKQLYSNLYEIIHGFRADTLKKPVFKDEDLIGFYEITIMRNNII
KGVNDATIKAIIIFILIFLTVLLCMIKFMNKKFNNPIQMLIKAMKAFPLGEESSIDYKATDEIGELINHFNYMKDEIIEK
NNEIEKQQKSKEYMIAAISHDLKTPLTSIRAYAELMNVEGEKNNKYVKGILNKCDYMTNMLQDLLMYTILSSNYNMEFVD
VEGEEFFEMLFSGYKELCKKNHINYLSEIKVNRNYKVDVKQMIRLVDNLIANAIIHTEKEKYICMGAISSQYELPNWVQG
DDREKINNFRKDHTVIIVKNQGDEISKENIKRIIEPFYKGDNSRNKRGGTGLGLSIVKLIIEKHGGEINILSSKKRGTLI
VCVLKEERNEFL

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=253, Percent_Identity=25.6916996047431, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789808, Length=275, Percent_Identity=26.5454545454545, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57787; Mature: 57656

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSRIQIYRRSVTMKIKNWITVSYITVMLIPILTAVMLFSWIKNYNRKTEMEDYIKNMQT
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FEKYDELLNNERLFSYATKKLNIIDEKDKDSMRIELYDKNGGKIYSSSSDEYFYPLPKKQ
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCEECCCCHHH
LYSNLYEIIHGFRADTLKKPVFKDEDLIGFYEITIMRNNIIKGVNDATIKAIIIFILIFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHH
TVLLCMIKFMNKKFNNPIQMLIKAMKAFPLGEESSIDYKATDEIGELINHFNYMKDEIIE
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KNNEIEKQQKSKEYMIAAISHDLKTPLTSIRAYAELMNVEGEKNNKYVKGILNKCDYMTN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MLQDLLMYTILSSNYNMEFVDVEGEEFFEMLFSGYKELCKKNHINYLSEIKVNRNYKVDV
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCCH
KQMIRLVDNLIANAIIHTEKEKYICMGAISSQYELPNWVQGDDREKINNFRKDHTVIIVK
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
NQGDEISKENIKRIIEPFYKGDNSRNKRGGTGLGLSIVKLIIEKHGGEINILSSKKRGTL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCE
IVCVLKEERNEFL
EEEEEECCCCCCC
>Mature Secondary Structure 
GSRIQIYRRSVTMKIKNWITVSYITVMLIPILTAVMLFSWIKNYNRKTEMEDYIKNMQT
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FEKYDELLNNERLFSYATKKLNIIDEKDKDSMRIELYDKNGGKIYSSSSDEYFYPLPKKQ
HHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCEECCCCHHH
LYSNLYEIIHGFRADTLKKPVFKDEDLIGFYEITIMRNNIIKGVNDATIKAIIIFILIFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHH
TVLLCMIKFMNKKFNNPIQMLIKAMKAFPLGEESSIDYKATDEIGELINHFNYMKDEIIE
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KNNEIEKQQKSKEYMIAAISHDLKTPLTSIRAYAELMNVEGEKNNKYVKGILNKCDYMTN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MLQDLLMYTILSSNYNMEFVDVEGEEFFEMLFSGYKELCKKNHINYLSEIKVNRNYKVDV
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCCH
KQMIRLVDNLIANAIIHTEKEKYICMGAISSQYELPNWVQGDDREKINNFRKDHTVIIVK
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEE
NQGDEISKENIKRIIEPFYKGDNSRNKRGGTGLGLSIVKLIIEKHGGEINILSSKKRGTL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCE
IVCVLKEERNEFL
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]