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The map label for this gene is argG

Identifier: 28210299

GI number: 28210299

Start: 580132

End: 581328

Strand: Direct

Name: argG

Synonym: CTC00561

Alternate gene names: 28210299

Gene position: 580132-581328 (Clockwise)

Preceding gene: 28210298

Following gene: 28210300

Centisome position: 20.72

GC content: 31.41

Gene sequence:

>1197_bases
ATGACAAAGGAAAAAGTAGTATTAGCATATTCAGGGGGCTTAGACACTTCTATAATCATACCTTGGTTAAAAGAGAATTA
TGATTTAGATGTTATTGCAGTATGTATAGATGTAGGACAAGATGATGATATGGAAGAAGTTAAGAAAAAAGCCATTAAAA
CTGGGGCAGTTAAGGTTTATGTAGAGGATGCAAAGGAAGAATTCGTAAAGGATTATGTTTTTAAAGCTTTAAAGGCAAAT
GCTCTTTATGAAGAAAAGTATATGTTGGGGACATCTCTTGCAAGACCGTTAATGGCTAAAAAACTTGTGGAAATTGCTCA
TAAAGAAGGAGCAAAATATATATGTCATGGATGTACAGGTAAGGGAAATGATCAGGTAAGATTTGAAGTTGGAATAGCAT
CTTTTGATCCAAGTATCAAAATAATAGCACCTTGGAGAATTTGGGATATAAAATCTAGAGAAGATGCTATAGATTATGCG
AAGGAAAAAGGCGTTGAAGTACCTGTAACTAAAAAGAAAATATACTCAGTAGATAAAAATATATTGCATACAAGTCATGA
AGGTGGAGAATTAGAAGATCCTAAAAATGCACATAATAAAGAAATGTATAGTATGGTTACACCACCAGAAAAAGCTAAAG
ATGAGCCTACTTATGTGGACATATATTTTAATAAGGGTGTACCTGAAAAAATTAATGGAAAAGAAATATCTCCTGTTGAA
CTTTTAAATACTTTAAATAAAATTGGTGGAGAAAATGGAGTAGGTGTAGTAGATATAGTAGAAAATAGATTGGTTGGTAT
GAAATCAAGAGGAGTTTATGAAACACCGGGGGGAACTATTCTATATGAAGCTCATAAAGATTTAGAGAGCCTTACTTTAG
ACAAATTAACTCTCCATTGTAAGCAAGAATTAGCTCAAAAATATGGTGAAATAGCTTATGATGGTTTATGGTTTACTACT
TTAAGAGAATCATTAGATGCCTTTGTGGATGTAACACAAGAAAATGTAACAGGAACAGTTAAGTTAAAACTATACAAAGG
GAATATAATGAATGCTGGAATAGATACTAAAAACGCTCTTTATGATGAAGGAATATCTTCCTTTGGAGCAAGTGAATTAT
ATAGTCATAAAGATGCAGAAGGATTTATTAAATTATTTTCCCTTCCTAGTAAAATAAAAGCTTTAAAGAATAAATAA

Upstream 100 bases:

>100_bases
GAACAAAGGAATTTCTGAAAAAAGTTTTATAATCAATGAAATATAAATCAAATATGATTTATTATAAATTTTAAAAGTAA
AATATAAGGGGGCTTTATAC

Downstream 100 bases:

>100_bases
TATTAAAAATTAAAGGGGCGGTCCTATTAAAAATTAAAAGTTAAAGGATTGTCCCTTATAAAATTAAATAAAAATGTAAT
ATAAAGGAGGGGTCTTATGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCIDVGQDDDMEEVKKKAIKTGAVKVYVEDAKEEFVKDYVFKALKAN
ALYEEKYMLGTSLARPLMAKKLVEIAHKEGAKYICHGCTGKGNDQVRFEVGIASFDPSIKIIAPWRIWDIKSREDAIDYA
KEKGVEVPVTKKKIYSVDKNILHTSHEGGELEDPKNAHNKEMYSMVTPPEKAKDEPTYVDIYFNKGVPEKINGKEISPVE
LLNTLNKIGGENGVGVVDIVENRLVGMKSRGVYETPGGTILYEAHKDLESLTLDKLTLHCKQELAQKYGEIAYDGLWFTT
LRESLDAFVDVTQENVTGTVKLKLYKGNIMNAGIDTKNALYDEGISSFGASELYSHKDAEGFIKLFSLPSKIKALKNK

Sequences:

>Translated_398_residues
MTKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCIDVGQDDDMEEVKKKAIKTGAVKVYVEDAKEEFVKDYVFKALKAN
ALYEEKYMLGTSLARPLMAKKLVEIAHKEGAKYICHGCTGKGNDQVRFEVGIASFDPSIKIIAPWRIWDIKSREDAIDYA
KEKGVEVPVTKKKIYSVDKNILHTSHEGGELEDPKNAHNKEMYSMVTPPEKAKDEPTYVDIYFNKGVPEKINGKEISPVE
LLNTLNKIGGENGVGVVDIVENRLVGMKSRGVYETPGGTILYEAHKDLESLTLDKLTLHCKQELAQKYGEIAYDGLWFTT
LRESLDAFVDVTQENVTGTVKLKLYKGNIMNAGIDTKNALYDEGISSFGASELYSHKDAEGFIKLFSLPSKIKALKNK
>Mature_397_residues
TKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCIDVGQDDDMEEVKKKAIKTGAVKVYVEDAKEEFVKDYVFKALKANA
LYEEKYMLGTSLARPLMAKKLVEIAHKEGAKYICHGCTGKGNDQVRFEVGIASFDPSIKIIAPWRIWDIKSREDAIDYAK
EKGVEVPVTKKKIYSVDKNILHTSHEGGELEDPKNAHNKEMYSMVTPPEKAKDEPTYVDIYFNKGVPEKINGKEISPVEL
LNTLNKIGGENGVGVVDIVENRLVGMKSRGVYETPGGTILYEAHKDLESLTLDKLTLHCKQELAQKYGEIAYDGLWFTTL
RESLDAFVDVTQENVTGTVKLKLYKGNIMNAGIDTKNALYDEGISSFGASELYSHKDAEGFIKLFSLPSKIKALKNK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=396, Percent_Identity=46.969696969697, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI16950633, Length=396, Percent_Identity=46.969696969697, Blast_Score=357, Evalue=1e-98,
Organism=Escherichia coli, GI1789563, Length=368, Percent_Identity=28.804347826087, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324514, Length=400, Percent_Identity=41.25, Blast_Score=310, Evalue=2e-85,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=44.4723618090452, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_CLOTE (P59602)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781243.1
- ProteinModelPortal:   P59602
- SMR:   P59602
- GeneID:   1058773
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00561
- NMPDR:   fig|212717.1.peg.465
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   P59602
- ProtClustDB:   PRK00509
- BioCyc:   CTET212717:CTC_00561-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44419; Mature: 44288

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 92-92 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 176-176 BINDING 185-185 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCIDVGQDDDMEEVKKKAIKTGAVKVY
CCCCEEEEEECCCCCCEEEEEHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCEEEEE
VEDAKEEFVKDYVFKALKANALYEEKYMLGTSLARPLMAKKLVEIAHKEGAKYICHGCTG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KGNDQVRFEVGIASFDPSIKIIAPWRIWDIKSREDAIDYAKEKGVEVPVTKKKIYSVDKN
CCCCEEEEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ILHTSHEGGELEDPKNAHNKEMYSMVTPPEKAKDEPTYVDIYFNKGVPEKINGKEISPVE
HHCCCCCCCCCCCCCCCCCHHHHEECCCCHHCCCCCCEEEEEECCCCCCCCCCCCCCHHH
LLNTLNKIGGENGVGVVDIVENRLVGMKSRGVYETPGGTILYEAHKDLESLTLDKLTLHC
HHHHHHHHCCCCCCEEEEEHHCHHCCCCCCCCEECCCCEEEECCHHCHHHHHHHHHHHHH
KQELAQKYGEIAYDGLWFTTLRESLDAFVDVTQENVTGTVKLKLYKGNIMNAGIDTKNAL
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEECCCCCCHHH
YDEGISSFGASELYSHKDAEGFIKLFSLPSKIKALKNK
HHHHHHHCCHHHHHCCCCHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TKEKVVLAYSGGLDTSIIIPWLKENYDLDVIAVCIDVGQDDDMEEVKKKAIKTGAVKVY
CCCEEEEEECCCCCCEEEEEHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCEEEEE
VEDAKEEFVKDYVFKALKANALYEEKYMLGTSLARPLMAKKLVEIAHKEGAKYICHGCTG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KGNDQVRFEVGIASFDPSIKIIAPWRIWDIKSREDAIDYAKEKGVEVPVTKKKIYSVDKN
CCCCEEEEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
ILHTSHEGGELEDPKNAHNKEMYSMVTPPEKAKDEPTYVDIYFNKGVPEKINGKEISPVE
HHCCCCCCCCCCCCCCCCCHHHHEECCCCHHCCCCCCEEEEEECCCCCCCCCCCCCCHHH
LLNTLNKIGGENGVGVVDIVENRLVGMKSRGVYETPGGTILYEAHKDLESLTLDKLTLHC
HHHHHHHHCCCCCCEEEEEHHCHHCCCCCCCCEECCCCEEEECCHHCHHHHHHHHHHHHH
KQELAQKYGEIAYDGLWFTTLRESLDAFVDVTQENVTGTVKLKLYKGNIMNAGIDTKNAL
HHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEECCCCCCHHH
YDEGISSFGASELYSHKDAEGFIKLFSLPSKIKALKNK
HHHHHHHCCHHHHHCCCCHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129