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The map label for this gene is yisS [H]

Identifier: 28210255

GI number: 28210255

Start: 520751

End: 521788

Strand: Direct

Name: yisS [H]

Synonym: CTC00513

Alternate gene names: 28210255

Gene position: 520751-521788 (Clockwise)

Preceding gene: 28210254

Following gene: 28210256

Centisome position: 18.6

GC content: 31.12

Gene sequence:

>1038_bases
ATGGAGGAAAATATGAATAAGATAAAGGTTGGCATTGTTGGTTTAGGAAGACTTGGAAGAAAACATGCAGAAAATATAGC
TTTAAGAATATCTAATGCAGAGCTTACTGCAGTTTGTAGTGTAATAAAAGATGAAGTTGATGAAGCTCAAAAAAAACTTA
ATGTTAAGTATGGATATACGGATTTTGATGAAATGATTAAAAATAAGGAATTAGATGCTATATTTATATCATCGCCATCA
GGATTTCATTGTTCACAAATAGAGAAAGCCCTAAAAGCAGGATTTCATGTATTTAGTGAAAAACCTCTTGGATTGCATTT
AGAAGAAACTAAAAAACTAGCTAAAGTAGTTAATGGATATAAGGATAAACAGATATTTATGCTTGGATTTATGAGAAGAT
ATGATAAATCCTACTTATATGCAAAGGAAAAGATAGAAGATGGGGAAATTGGTAAACCAATGCTTATAAGATGCTATGGA
TTAGATCCATCTTGTTCTATAGAAAGTTTTTTGAATTTTGCTAAAAATAATTATAGTGGTGGGTTATTTTTAGATATGGC
TATACATGATTTAGATCTTGCAAGATGGTATTTAAATTCAGAAGCTGAAAAGGTGTGGGCTATAGGAGATGCCTATGAGT
ATGAAGACTTTAAAGAAATTAATGATGCAGAAACAGGAGCAGCCCTAGTTAAATTTAAAAATGGAACAATGGGTATATTT
GTGGCAGGAAGAAATTGTGCCCATGGATATCATATAGAAACAGAAATAATAGGCACAAAAGGTACTTTAAGAATAGGAAC
CATACCAGAGAAGAATATGGTTACTGTATTTAATGATAAGGGGGCTATAAGAGAGTGTTCTCAAGGATTCTTAGAAAGAT
TTGAACAAGCATATTTATCTGAAACAGAAGATTTTATAAATTGTATAATGGAAAAAAGACAACCTAAAATAACTGTAGAA
GATGGCGTGAATTCAACAGCCTTAGCCTATGCATGTAAGGAATCTTTTGAAACCGGAAAGTTAATAGAATTAAAATAG

Upstream 100 bases:

>100_bases
AGCAAGACCCTGCAAGGGCAAATCCACTTGAATATGCTATAAAAGCTAGAAAGTATATACAGGAAAAGGCAGCGATTTAA
TCAATATCTTACAGATTTTA

Downstream 100 bases:

>100_bases
TAGTTATAAAAAAAGGGCATATTACTTTCAAATATAACCATATGAAAGCAGTATGTCCTATAAAATAATATAAATGTTGA
AAATTTATATAAACACTATA

Product: myo-inositol 2-dehydrogenase

Products: 2,4,6/3,5-pentahydroxycyclohexanone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEENMNKIKVGIVGLGRLGRKHAENIALRISNAELTAVCSVIKDEVDEAQKKLNVKYGYTDFDEMIKNKELDAIFISSPS
GFHCSQIEKALKAGFHVFSEKPLGLHLEETKKLAKVVNGYKDKQIFMLGFMRRYDKSYLYAKEKIEDGEIGKPMLIRCYG
LDPSCSIESFLNFAKNNYSGGLFLDMAIHDLDLARWYLNSEAEKVWAIGDAYEYEDFKEINDAETGAALVKFKNGTMGIF
VAGRNCAHGYHIETEIIGTKGTLRIGTIPEKNMVTVFNDKGAIRECSQGFLERFEQAYLSETEDFINCIMEKRQPKITVE
DGVNSTALAYACKESFETGKLIELK

Sequences:

>Translated_345_residues
MEENMNKIKVGIVGLGRLGRKHAENIALRISNAELTAVCSVIKDEVDEAQKKLNVKYGYTDFDEMIKNKELDAIFISSPS
GFHCSQIEKALKAGFHVFSEKPLGLHLEETKKLAKVVNGYKDKQIFMLGFMRRYDKSYLYAKEKIEDGEIGKPMLIRCYG
LDPSCSIESFLNFAKNNYSGGLFLDMAIHDLDLARWYLNSEAEKVWAIGDAYEYEDFKEINDAETGAALVKFKNGTMGIF
VAGRNCAHGYHIETEIIGTKGTLRIGTIPEKNMVTVFNDKGAIRECSQGFLERFEQAYLSETEDFINCIMEKRQPKITVE
DGVNSTALAYACKESFETGKLIELK
>Mature_345_residues
MEENMNKIKVGIVGLGRLGRKHAENIALRISNAELTAVCSVIKDEVDEAQKKLNVKYGYTDFDEMIKNKELDAIFISSPS
GFHCSQIEKALKAGFHVFSEKPLGLHLEETKKLAKVVNGYKDKQIFMLGFMRRYDKSYLYAKEKIEDGEIGKPMLIRCYG
LDPSCSIESFLNFAKNNYSGGLFLDMAIHDLDLARWYLNSEAEKVWAIGDAYEYEDFKEINDAETGAALVKFKNGTMGIF
VAGRNCAHGYHIETEIIGTKGTLRIGTIPEKNMVTVFNDKGAIRECSQGFLERFEQAYLSETEDFINCIMEKRQPKITVE
DGVNSTALAYACKESFETGKLIELK

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=205, Percent_Identity=27.8048780487805, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082405, Length=261, Percent_Identity=24.904214559387, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.1.1.18

Molecular weight: Translated: 38914; Mature: 38914

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENMNKIKVGIVGLGRLGRKHAENIALRISNAELTAVCSVIKDEVDEAQKKLNVKYGYT
CCCCCCEEEEEEEECCHHCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCC
DFDEMIKNKELDAIFISSPSGFHCSQIEKALKAGFHVFSEKPLGLHLEETKKLAKVVNGY
CHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
KDKQIFMLGFMRRYDKSYLYAKEKIEDGEIGKPMLIRCYGLDPSCSIESFLNFAKNNYSG
CCCCHHHHHHHHHHCHHHEEHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
GLFLDMAIHDLDLARWYLNSEAEKVWAIGDAYEYEDFKEINDAETGAALVKFKNGTMGIF
CEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCCCCEEEEEEECCCEEEE
VAGRNCAHGYHIETEIIGTKGTLRIGTIPEKNMVTVFNDKGAIRECSQGFLERFEQAYLS
EECCCCCCCEEEEEEEECCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
ETEDFINCIMEKRQPKITVEDGVNSTALAYACKESFETGKLIELK
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MEENMNKIKVGIVGLGRLGRKHAENIALRISNAELTAVCSVIKDEVDEAQKKLNVKYGYT
CCCCCCEEEEEEEECCHHCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCC
DFDEMIKNKELDAIFISSPSGFHCSQIEKALKAGFHVFSEKPLGLHLEETKKLAKVVNGY
CHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
KDKQIFMLGFMRRYDKSYLYAKEKIEDGEIGKPMLIRCYGLDPSCSIESFLNFAKNNYSG
CCCCHHHHHHHHHHCHHHEEHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
GLFLDMAIHDLDLARWYLNSEAEKVWAIGDAYEYEDFKEINDAETGAALVKFKNGTMGIF
CEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCCCCEEEEEEECCCEEEE
VAGRNCAHGYHIETEIIGTKGTLRIGTIPEKNMVTVFNDKGAIRECSQGFLERFEQAYLS
EECCCCCCCEEEEEEEECCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
ETEDFINCIMEKRQPKITVEDGVNSTALAYACKESFETGKLIELK
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: myo-inositol; NAD+

Specific reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377; 8407808 [H]