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The map label for this gene is iolE

Identifier: 28210254

GI number: 28210254

Start: 519834

End: 520730

Strand: Direct

Name: iolE

Synonym: CTC00512

Alternate gene names: 28210254

Gene position: 519834-520730 (Clockwise)

Preceding gene: 28210253

Following gene: 28210255

Centisome position: 18.57

GC content: 32.44

Gene sequence:

>897_bases
ATGTTTAATAAAGATAAAGTGAAAATTGGAATAGCGCCTATAGCATGGACTAATGATGATATGCCAGAACTTGGAGCAGA
AAACACTTTTGAACAATGTATAAGTGAAATGGCATTGTCAGGTTTTAAAGGAACAGAAGTGGGGAATAAATACCCAAGGG
ATACTAAAGTTCTTAAAAGGGCTTTAGAGCTTAGAGATATGCAAATTGCTAGTGCATGGTTTAGTGCTTTTTTAACTACA
AAACCTTATGAAGAAACAGAAAAAGCTTTTATAAAACACAGGGATTTTCTTTATGAAATGAGATCAAAGGTAATAGTTAT
ATCAGAACAAGGACATAGTATACAAGGGAAAATGGAAACACCTATATTTGAAGAAAAACCTATATTTACAGAAGAAGAGT
GGAAAGATTTAGCTAAAGGTTTAAATAAGCTTGGAAAACTTGCAAAAGAAAAAAATATGAAAGTAGTTTATCATCATCAT
ATGGGAACGGGCGTTCAAACTACAGAAGAAATAGATAAACTGATGGAAATGACAGATCCTACACTTGTATATCTATTATA
TGACACTGGGCATCTTGTATTTTCCGGAGAAGATCCTATAGTTGTTTTAAGAAAATATATAAATAGAATAAAGCATGTTC
ACTTGAAAGATATAAGAGAAGATGTAGTAAAGATAGTAAAGCAAAAGAAGATGAGTTTTTTACAGGCTGTTAAATTAGGA
GCATTTACTGTTCCAGGAGATGGAGATATAGACTTTAAACCCGTATTTAACATATTAGCAGAGAATAATTATGAAGGATG
GCTTTTAGTAGAAGCAGAGCAAGACCCTGCAAGGGCAAATCCACTTGAATATGCTATAAAAGCTAGAAAGTATATACAGG
AAAAGGCAGCGATTTAA

Upstream 100 bases:

>100_bases
ATAGGAATTGCTGCTACAAAGTCATTAAAAGAAGGAAGAGCAGTTAAGATCTCTGAAATAGGTTTATAACAAATCAGATT
AAAGGATTGGGGGAAAATTT

Downstream 100 bases:

>100_bases
TCAATATCTTACAGATTTTAATGGAGGAAAATATGAATAAGATAAAGGTTGGCATTGTTGGTTTAGGAAGACTTGGAAGA
AAACATGCAGAAAATATAGC

Product: myo-inositol catabolism protein iolE

Products: NA

Alternate protein names: 2-keto-myo-inositol dehydratase; 2KMI dehydratase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MFNKDKVKIGIAPIAWTNDDMPELGAENTFEQCISEMALSGFKGTEVGNKYPRDTKVLKRALELRDMQIASAWFSAFLTT
KPYEETEKAFIKHRDFLYEMRSKVIVISEQGHSIQGKMETPIFEEKPIFTEEEWKDLAKGLNKLGKLAKEKNMKVVYHHH
MGTGVQTTEEIDKLMEMTDPTLVYLLYDTGHLVFSGEDPIVVLRKYINRIKHVHLKDIREDVVKIVKQKKMSFLQAVKLG
AFTVPGDGDIDFKPVFNILAENNYEGWLLVEAEQDPARANPLEYAIKARKYIQEKAAI

Sequences:

>Translated_298_residues
MFNKDKVKIGIAPIAWTNDDMPELGAENTFEQCISEMALSGFKGTEVGNKYPRDTKVLKRALELRDMQIASAWFSAFLTT
KPYEETEKAFIKHRDFLYEMRSKVIVISEQGHSIQGKMETPIFEEKPIFTEEEWKDLAKGLNKLGKLAKEKNMKVVYHHH
MGTGVQTTEEIDKLMEMTDPTLVYLLYDTGHLVFSGEDPIVVLRKYINRIKHVHLKDIREDVVKIVKQKKMSFLQAVKLG
AFTVPGDGDIDFKPVFNILAENNYEGWLLVEAEQDPARANPLEYAIKARKYIQEKAAI
>Mature_298_residues
MFNKDKVKIGIAPIAWTNDDMPELGAENTFEQCISEMALSGFKGTEVGNKYPRDTKVLKRALELRDMQIASAWFSAFLTT
KPYEETEKAFIKHRDFLYEMRSKVIVISEQGHSIQGKMETPIFEEKPIFTEEEWKDLAKGLNKLGKLAKEKNMKVVYHHH
MGTGVQTTEEIDKLMEMTDPTLVYLLYDTGHLVFSGEDPIVVLRKYINRIKHVHLKDIREDVVKIVKQKKMSFLQAVKLG
AFTVPGDGDIDFKPVFNILAENNYEGWLLVEAEQDPARANPLEYAIKARKYIQEKAAI

Specific function: Catalyzes the dehydration of inosose (2-keto-myo- inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi- inositol)

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iolE/mocC family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLE_CLOTE (Q898E6)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781198.1
- ProteinModelPortal:   Q898E6
- SMR:   Q898E6
- GeneID:   1060116
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00512
- NMPDR:   fig|212717.1.peg.420
- HOGENOM:   HBG654552
- OMA:   DLGKENT
- ProtClustDB:   CLSK810783
- BioCyc:   CTET212717:CTC_00512-MONOMER
- HAMAP:   MF_01672
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =4.2.1.44

Molecular weight: Translated: 34217; Mature: 34217

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKDKVKIGIAPIAWTNDDMPELGAENTFEQCISEMALSGFKGTEVGNKYPRDTKVLKR
CCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
ALELRDMQIASAWFSAFLTTKPYEETEKAFIKHRDFLYEMRSKVIVISEQGHSIQGKMET
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
PIFEEKPIFTEEEWKDLAKGLNKLGKLAKEKNMKVVYHHHMGTGVQTTEEIDKLMEMTDP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
TLVYLLYDTGHLVFSGEDPIVVLRKYINRIKHVHLKDIREDVVKIVKQKKMSFLQAVKLG
EEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AFTVPGDGDIDFKPVFNILAENNYEGWLLVEAEQDPARANPLEYAIKARKYIQEKAAI
CEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNKDKVKIGIAPIAWTNDDMPELGAENTFEQCISEMALSGFKGTEVGNKYPRDTKVLKR
CCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
ALELRDMQIASAWFSAFLTTKPYEETEKAFIKHRDFLYEMRSKVIVISEQGHSIQGKMET
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
PIFEEKPIFTEEEWKDLAKGLNKLGKLAKEKNMKVVYHHHMGTGVQTTEEIDKLMEMTDP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCC
TLVYLLYDTGHLVFSGEDPIVVLRKYINRIKHVHLKDIREDVVKIVKQKKMSFLQAVKLG
EEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AFTVPGDGDIDFKPVFNILAENNYEGWLLVEAEQDPARANPLEYAIKARKYIQEKAAI
CEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129