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The map label for this gene is mscS [H]

Identifier: 28210229

GI number: 28210229

Start: 486492

End: 487301

Strand: Direct

Name: mscS [H]

Synonym: CTC00481

Alternate gene names: 28210229

Gene position: 486492-487301 (Clockwise)

Preceding gene: 28210228

Following gene: 28210230

Centisome position: 17.38

GC content: 28.15

Gene sequence:

>810_bases
ATGGATTTTGATTATTTTAATCAGATTATAGTATTGTACGGAAGTAAAATAATATTAGCAGTTGCAACATTAGTTGTAGG
ATTATATGCAATTAAAAAGATAGTAGGACTTTTATCTAGAAGATTTTCTAAAATAGGATTAGAAAAATCTCTACAGTCTT
TTTCAATATCCTTTACTAGTATAGGATTAAAAATATTACTTGCTGTAACAATTGCTTCAATGCTAGGAGCAGAAATGACT
TCTTTTGTTGCAATTCTTGGTTCTGCAGGATTAGCAGTTGGACTAGCATTACAGGGAAGTTTGTCAAATTTTGCAGGAGG
AGTTCTTATTCTAATATTAAAGCCTTTTAAAGTAGGAGATTACATAGAAGCTGCAGGTCATAGTGGAACTGTATCTGAAA
TACAAGTTTTTTATACTATATTAAATACGCCAGATAATAAAAAGGTAATAATTCCAAATTCCAATATATCTAATGGCAGT
GTCACAAATTATTCTGCTAATTCTACAAGAAGAGTAGATTTGATTTTTAGCATAGATTATGATGAGAATATAGAAAGAGT
AAAAAAGGTTTTAATAAATATTATAGAGAGCAATTCTTTAATATTAAAAGAACCAGATCCTCAAATATTAGTGGGAGAAC
ATGGAGATAGTTCAGTAAATTTATATGTTAGAGTTTGGTGTGAAAATAAGGATTATTGGAATATATATTTTGGATTAATG
GAAAAAGTAAAAGTAGAATTTGATAAAGAATCTATAAATATACCATATCCTCAAGTAGAAATACAATATAAAGGACAAAA
GGCACAGTAG

Upstream 100 bases:

>100_bases
ATAATAATGTTCATCATTTTAATGTTAACAAGATATTCTTATGTGATATTTTGCTAAATCGTAAAGGAATATTAAATATT
TAAAAGAAAGGGATGATTAA

Downstream 100 bases:

>100_bases
TAGTGTCTTTTTTTACGAGTTAAATGTTAAATAAATAACATGAATATTCTGGATTTTATCAGGAAAATATTATAAAATAT
ATAGTATAATCCTGTATTCT

Product: putative mechanosensitive ion-channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MDFDYFNQIIVLYGSKIILAVATLVVGLYAIKKIVGLLSRRFSKIGLEKSLQSFSISFTSIGLKILLAVTIASMLGAEMT
SFVAILGSAGLAVGLALQGSLSNFAGGVLILILKPFKVGDYIEAAGHSGTVSEIQVFYTILNTPDNKKVIIPNSNISNGS
VTNYSANSTRRVDLIFSIDYDENIERVKKVLINIIESNSLILKEPDPQILVGEHGDSSVNLYVRVWCENKDYWNIYFGLM
EKVKVEFDKESINIPYPQVEIQYKGQKAQ

Sequences:

>Translated_269_residues
MDFDYFNQIIVLYGSKIILAVATLVVGLYAIKKIVGLLSRRFSKIGLEKSLQSFSISFTSIGLKILLAVTIASMLGAEMT
SFVAILGSAGLAVGLALQGSLSNFAGGVLILILKPFKVGDYIEAAGHSGTVSEIQVFYTILNTPDNKKVIIPNSNISNGS
VTNYSANSTRRVDLIFSIDYDENIERVKKVLINIIESNSLILKEPDPQILVGEHGDSSVNLYVRVWCENKDYWNIYFGLM
EKVKVEFDKESINIPYPQVEIQYKGQKAQ
>Mature_269_residues
MDFDYFNQIIVLYGSKIILAVATLVVGLYAIKKIVGLLSRRFSKIGLEKSLQSFSISFTSIGLKILLAVTIASMLGAEMT
SFVAILGSAGLAVGLALQGSLSNFAGGVLILILKPFKVGDYIEAAGHSGTVSEIQVFYTILNTPDNKKVIIPNSNISNGS
VTNYSANSTRRVDLIFSIDYDENIERVKKVLINIIESNSLILKEPDPQILVGEHGDSSVNLYVRVWCENKDYWNIYFGLM
EKVKVEFDKESINIPYPQVEIQYKGQKAQ

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=262, Percent_Identity=37.7862595419847, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786670, Length=261, Percent_Identity=26.8199233716475, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI2367355, Length=192, Percent_Identity=24.4791666666667, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787591, Length=210, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29672; Mature: 29672

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00064 L_LDH ; PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDYFNQIIVLYGSKIILAVATLVVGLYAIKKIVGLLSRRFSKIGLEKSLQSFSISFTS
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHH
IGLKILLAVTIASMLGAEMTSFVAILGSAGLAVGLALQGSLSNFAGGVLILILKPFKVGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCEEEEEEECCCCCC
YIEAAGHSGTVSEIQVFYTILNTPDNKKVIIPNSNISNGSVTNYSANSTRRVDLIFSIDY
HHHHCCCCCCHHHHEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEEEECC
DENIERVKKVLINIIESNSLILKEPDPQILVGEHGDSSVNLYVRVWCENKDYWNIYFGLM
CCCHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEEEEEEEECCCCEEEHHHHHH
EKVKVEFDKESINIPYPQVEIQYKGQKAQ
HHHEEEECCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MDFDYFNQIIVLYGSKIILAVATLVVGLYAIKKIVGLLSRRFSKIGLEKSLQSFSISFTS
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHH
IGLKILLAVTIASMLGAEMTSFVAILGSAGLAVGLALQGSLSNFAGGVLILILKPFKVGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCCEEEEEEECCCCCC
YIEAAGHSGTVSEIQVFYTILNTPDNKKVIIPNSNISNGSVTNYSANSTRRVDLIFSIDY
HHHHCCCCCCHHHHEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEEEECC
DENIERVKKVLINIIESNSLILKEPDPQILVGEHGDSSVNLYVRVWCENKDYWNIYFGLM
CCCHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCEEEEEEEEECCCCEEEHHHHHH
EKVKVEFDKESINIPYPQVEIQYKGQKAQ
HHHEEEECCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]