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The map label for this gene is gap [H]

Identifier: 28210134

GI number: 28210134

Start: 367595

End: 368602

Strand: Direct

Name: gap [H]

Synonym: CTC00378

Alternate gene names: 28210134

Gene position: 367595-368602 (Clockwise)

Preceding gene: 28210133

Following gene: 28210135

Centisome position: 13.13

GC content: 31.75

Gene sequence:

>1008_bases
ATGGTAAAGGTAGCAATTAACGGTTTTGGAAGAATAGGAAGAAATGTATTTAAGGTTTTAATTGAAAAATACAGTAGTGA
ATTGGAAATTGTTGGGATTAATGATTTAACTGATGCAAAAACTTTAGCTCATCTTTTAAAATATGATTCATTATATGGTA
AATTCAATGGAACAGTAGAAGCTAAAGAAAATGCTATAGCTATAAATGGCAAAGAAATAAAAATATTTGCAGAAAGAGAT
CCTAAAAATCTTCCATGGAAAGAAGAAGGAGTAGACATAGTTTTAGAATGCACAGGTTTATTTACAGACGCTACAAAAGC
TAAAGCACATATAGAAGCAGGAGCTAAAAAAGTAATTATTTCAGCACCAGCTAAAAATGAAGATGTTACTATAGTTTTAG
GTGTTAATGAAGAAAAATATAATTCAGAAGAACACAATATAATTTCAAATGCTTCTTGTACTACAAATTGTCTAGCACCT
TTTGCTAAAGTACTAGATCAAGAATTTGGAATAGTAGAAGGACTAATGACTACAATTCATTCCTATACTAATGACCAAAA
AATATTAGATGCTCCACATAAAGATTTAAGAAGAGCAAGAGCAGCAGCTGAATCAATGATTCCAACAACAACAGGAGCTG
CTAAAGCAGTTGCATTAGTTCTTCCACAATTAAAAGGAAAATTAAACGGAATGGCTGTAAGAGTTCCAACACCAACAGTA
TCAATGACTGATCTTGTTTGTACAATTTCAAAAGAGGCTACAGCAGAAGAAATAAATGCAGCTTTCAAAAAAGCTTCAGA
AACAGACATGAAAGGAATTCTTGGATATAGTGAAGAGCCATTAGTATCTGTAGACTATAGAGGAGATGCAAGATCTTCAA
TAGTTGATGGATTATCCACAATGGTAATGGGAAATAGATTAGTTAAAGTTGTTTCATGGTATGATAACGAATGGGGTTAT
TCAACAAGATTAGCTGATTTAGCTAAATATGTTGCTGATAGACTATAA

Upstream 100 bases:

>100_bases
TTTGATTTAAGTAGCATACAAAAGCAAAACGCTTTTAAGTATTAATATATCTATAATTCATCAAATTTAAAACTTTTAAA
AATTTAAGGAGGTCTTTTAA

Downstream 100 bases:

>100_bases
TCGAGTAAATAAGTCATACTTATAGTTAAAAACTAAGGGGGTTTTTGCTTTGTAGTAAAGCTATAAGACAAAACCCTCTT
TTGCCTATATAAAGAAAGGG

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MVKVAINGFGRIGRNVFKVLIEKYSSELEIVGINDLTDAKTLAHLLKYDSLYGKFNGTVEAKENAIAINGKEIKIFAERD
PKNLPWKEEGVDIVLECTGLFTDATKAKAHIEAGAKKVIISAPAKNEDVTIVLGVNEEKYNSEEHNIISNASCTTNCLAP
FAKVLDQEFGIVEGLMTTIHSYTNDQKILDAPHKDLRRARAAAESMIPTTTGAAKAVALVLPQLKGKLNGMAVRVPTPTV
SMTDLVCTISKEATAEEINAAFKKASETDMKGILGYSEEPLVSVDYRGDARSSIVDGLSTMVMGNRLVKVVSWYDNEWGY
STRLADLAKYVADRL

Sequences:

>Translated_335_residues
MVKVAINGFGRIGRNVFKVLIEKYSSELEIVGINDLTDAKTLAHLLKYDSLYGKFNGTVEAKENAIAINGKEIKIFAERD
PKNLPWKEEGVDIVLECTGLFTDATKAKAHIEAGAKKVIISAPAKNEDVTIVLGVNEEKYNSEEHNIISNASCTTNCLAP
FAKVLDQEFGIVEGLMTTIHSYTNDQKILDAPHKDLRRARAAAESMIPTTTGAAKAVALVLPQLKGKLNGMAVRVPTPTV
SMTDLVCTISKEATAEEINAAFKKASETDMKGILGYSEEPLVSVDYRGDARSSIVDGLSTMVMGNRLVKVVSWYDNEWGY
STRLADLAKYVADRL
>Mature_335_residues
MVKVAINGFGRIGRNVFKVLIEKYSSELEIVGINDLTDAKTLAHLLKYDSLYGKFNGTVEAKENAIAINGKEIKIFAERD
PKNLPWKEEGVDIVLECTGLFTDATKAKAHIEAGAKKVIISAPAKNEDVTIVLGVNEEKYNSEEHNIISNASCTTNCLAP
FAKVLDQEFGIVEGLMTTIHSYTNDQKILDAPHKDLRRARAAAESMIPTTTGAAKAVALVLPQLKGKLNGMAVRVPTPTV
SMTDLVCTISKEATAEEINAAFKKASETDMKGILGYSEEPLVSVDYRGDARSSIVDGLSTMVMGNRLVKVVSWYDNEWGY
STRLADLAKYVADRL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=335, Percent_Identity=49.8507462686567, Blast_Score=332, Evalue=2e-91,
Organism=Homo sapiens, GI7657116, Length=333, Percent_Identity=47.4474474474475, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=53.0120481927711, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789295, Length=327, Percent_Identity=47.0948012232416, Blast_Score=323, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI32566163, Length=338, Percent_Identity=48.8165680473373, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17568413, Length=338, Percent_Identity=48.8165680473373, Blast_Score=319, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17534677, Length=338, Percent_Identity=47.6331360946746, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI17534679, Length=338, Percent_Identity=47.9289940828402, Blast_Score=317, Evalue=7e-87,
Organism=Saccharomyces cerevisiae, GI6321631, Length=334, Percent_Identity=51.1976047904192, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=50.8982035928144, Blast_Score=342, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6322409, Length=334, Percent_Identity=49.4011976047904, Blast_Score=338, Evalue=8e-94,
Organism=Drosophila melanogaster, GI85725000, Length=336, Percent_Identity=49.1071428571429, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI22023983, Length=336, Percent_Identity=49.1071428571429, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI17933600, Length=336, Percent_Identity=47.6190476190476, Blast_Score=324, Evalue=5e-89,
Organism=Drosophila melanogaster, GI18110149, Length=336, Percent_Identity=47.6190476190476, Blast_Score=324, Evalue=5e-89,
Organism=Drosophila melanogaster, GI19922412, Length=333, Percent_Identity=48.3483483483483, Blast_Score=310, Evalue=7e-85,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36532; Mature: 36532

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVAINGFGRIGRNVFKVLIEKYSSELEIVGINDLTDAKTLAHLLKYDSLYGKFNGTVE
CEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
AKENAIAINGKEIKIFAERDPKNLPWKEEGVDIVLECTGLFTDATKAKAHIEAGAKKVII
ECCCEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEE
SAPAKNEDVTIVLGVNEEKYNSEEHNIISNASCTTNCLAPFAKVLDQEFGIVEGLMTTIH
ECCCCCCCEEEEEECCHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SYTNDQKILDAPHKDLRRARAAAESMIPTTTGAAKAVALVLPQLKGKLNGMAVRVPTPTV
HHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
SMTDLVCTISKEATAEEINAAFKKASETDMKGILGYSEEPLVSVDYRGDARSSIVDGLST
CHHHHEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
MVMGNRLVKVVSWYDNEWGYSTRLADLAKYVADRL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKVAINGFGRIGRNVFKVLIEKYSSELEIVGINDLTDAKTLAHLLKYDSLYGKFNGTVE
CEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
AKENAIAINGKEIKIFAERDPKNLPWKEEGVDIVLECTGLFTDATKAKAHIEAGAKKVII
ECCCEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEE
SAPAKNEDVTIVLGVNEEKYNSEEHNIISNASCTTNCLAPFAKVLDQEFGIVEGLMTTIH
ECCCCCCCEEEEEECCHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SYTNDQKILDAPHKDLRRARAAAESMIPTTTGAAKAVALVLPQLKGKLNGMAVRVPTPTV
HHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
SMTDLVCTISKEATAEEINAAFKKASETDMKGILGYSEEPLVSVDYRGDARSSIVDGLST
CHHHHEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
MVMGNRLVKVVSWYDNEWGYSTRLADLAKYVADRL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]