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The map label for this gene is metK

Identifier: 28210081

GI number: 28210081

Start: 303526

End: 304701

Strand: Direct

Name: metK

Synonym: CTC00321

Alternate gene names: 28210081

Gene position: 303526-304701 (Clockwise)

Preceding gene: 28210078

Following gene: 28210082

Centisome position: 10.84

GC content: 33.93

Gene sequence:

>1176_bases
ATGAGGAAATTATTTACATCTGAATCAGTTACAGAAGGGCATCCAGATAAGATTTGCGATCAAATTTCCGACGCAGTGCT
TGATGCCATACTTGAAAGGGACCCAAATGGTAGAGTTGCTTGTGAAACTAGTGTAACTACAGGAATGGTTTTGGTGACAG
GAGAGATAACTACTAATTGTTATGTAGATATACCTAAAATAGTTAGAAATACAATAGAAGCTATAGGATATACTAGGGCT
AAATATGGATTTGATTCAGATACATGTGCAGTGCTAACATCTATATCGGAACAATCTCCAGATATAGCTATGGGAGTTGA
TGAATCTCTAGAATCTAAGCAGGGTGGAATGGATAAGGAAGATGTCATAGGAGCAGGAGATCAAGGAATGATGTTTGGAT
ATGCAAATAATGAAACATCAGAATATATGCCAATGGCAATATCTATAGCACATAAATTATCTAGAAGATTATCTGAAGTA
AGAAAAAATGGAACATTACCTTACTTAAGACCAGATGGAAAAACACAAGTTACCGTAGAATATGAAGATAATAAAGCAAT
AAGAATAGATACTATCGTAGTATCTACTCAACATGGACCAGAAGTTACAAGGGAACAAATAGAGAAAGATATTTTTGAAC
ATGTAATATCTAAAGTTGTGCCAGTAGAATTTTTAGATGAAAACACTAATTATTATATAAATCCAACAGGAAGATTTGTT
GTAGGCGGACCACAAGGTGATGCTGGATTAACTGGAAGAAAAATAATAGTTGATACATATGGCGGATATGGAAGGCATGG
TGGGGGAGCATTTTCTGGAAAAGATCCTACAAAGGTTGATAGGTCCGCAGCATATGCAGCTAGATGGGTAGCTAAAAATC
TTGTAGCAGCAGGGGTAGCGGATAAGTTAGAAGTTCAATTAGCTTATGCTATAGGAATAGCAAAACCTGTATCTATTTCA
GTAGAAACTTTTGGAACAGGAAAAATAGATGATAGAAAAATCGTAGAAATAATTAATAAAGTATTTGATTTAAGACCTTC
AGCTATAATAAAAGAATTAAACTTAAGAAGACCTATATATAAACAAACAGCAGCTTATGGACACTTTGGAAGAACAGATG
TAGAACTTCCATGGGAGAGTCTAAATAAATTAGAAGAAATAAAAAATTTAATTTAA

Upstream 100 bases:

>100_bases
AGTTCTGCAAGATAAGAGGGCTGGCAAATAAAAAGCAAGTCTCTTCTTATCGGAAGAGGCTTTATTTTTATATAAGAGCT
ACACAATAGGAGGTAAAAGG

Downstream 100 bases:

>100_bases
TATAATAGCATGTTAAAACCCTGGTGAAATTTTACCAGGGTTTTTTAACTTTCCAAAAGTATAATAAGGATTAGAAAATA
TAATATATGATATAATATAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILERDPNGRVACETSVTTGMVLVTGEITTNCYVDIPKIVRNTIEAIGYTRA
KYGFDSDTCAVLTSISEQSPDIAMGVDESLESKQGGMDKEDVIGAGDQGMMFGYANNETSEYMPMAISIAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYEDNKAIRIDTIVVSTQHGPEVTREQIEKDIFEHVISKVVPVEFLDENTNYYINPTGRFV
VGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEVQLAYAIGIAKPVSIS
VETFGTGKIDDRKIVEIINKVFDLRPSAIIKELNLRRPIYKQTAAYGHFGRTDVELPWESLNKLEEIKNLI

Sequences:

>Translated_391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILERDPNGRVACETSVTTGMVLVTGEITTNCYVDIPKIVRNTIEAIGYTRA
KYGFDSDTCAVLTSISEQSPDIAMGVDESLESKQGGMDKEDVIGAGDQGMMFGYANNETSEYMPMAISIAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYEDNKAIRIDTIVVSTQHGPEVTREQIEKDIFEHVISKVVPVEFLDENTNYYINPTGRFV
VGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEVQLAYAIGIAKPVSIS
VETFGTGKIDDRKIVEIINKVFDLRPSAIIKELNLRRPIYKQTAAYGHFGRTDVELPWESLNKLEEIKNLI
>Mature_391_residues
MRKLFTSESVTEGHPDKICDQISDAVLDAILERDPNGRVACETSVTTGMVLVTGEITTNCYVDIPKIVRNTIEAIGYTRA
KYGFDSDTCAVLTSISEQSPDIAMGVDESLESKQGGMDKEDVIGAGDQGMMFGYANNETSEYMPMAISIAHKLSRRLSEV
RKNGTLPYLRPDGKTQVTVEYEDNKAIRIDTIVVSTQHGPEVTREQIEKDIFEHVISKVVPVEFLDENTNYYINPTGRFV
VGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKLEVQLAYAIGIAKPVSIS
VETFGTGKIDDRKIVEIINKVFDLRPSAIIKELNLRRPIYKQTAAYGHFGRTDVELPWESLNKLEEIKNLI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=386, Percent_Identity=58.2901554404145, Blast_Score=447, Evalue=1e-126,
Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=58.1395348837209, Blast_Score=445, Evalue=1e-125,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=59.5360824742268, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=58.2901554404145, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17538494, Length=387, Percent_Identity=54.7803617571059, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=386, Percent_Identity=54.9222797927461, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.3571428571429, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32565905, Length=387, Percent_Identity=49.8708010335917, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=341, Percent_Identity=52.1994134897361, Blast_Score=354, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=347, Percent_Identity=52.7377521613833, Blast_Score=353, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=314, Percent_Identity=52.2292993630573, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=60.4938271604938, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=78.9473684210526, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=59.0673575129534, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320710, Length=392, Percent_Identity=58.9285714285714, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI45552159, Length=386, Percent_Identity=54.6632124352332, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580529, Length=386, Percent_Identity=54.6632124352332, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580537, Length=386, Percent_Identity=54.6632124352332, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580533, Length=386, Percent_Identity=54.6632124352332, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580535, Length=386, Percent_Identity=54.6632124352332, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580531, Length=386, Percent_Identity=54.6632124352332, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=54.4041450777202, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=54.4041450777202, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=53.2085561497326, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=55.2, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CLOTE (Q898W7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781025.1
- ProteinModelPortal:   Q898W7
- SMR:   Q898W7
- GeneID:   1059897
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00321
- NMPDR:   fig|212717.1.peg.247
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q898W7
- ProtClustDB:   PRK05250
- BioCyc:   CTET212717:CTC_00321-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42919; Mature: 42919

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFTSESVTEGHPDKICDQISDAVLDAILERDPNGRVACETSVTTGMVLVTGEITTNC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCE
YVDIPKIVRNTIEAIGYTRAKYGFDSDTCAVLTSISEQSPDIAMGVDESLESKQGGMDKE
EEECHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCHH
DVIGAGDQGMMFGYANNETSEYMPMAISIAHKLSRRLSEVRKNGTLPYLRPDGKTQVTVE
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YEDNKAIRIDTIVVSTQHGPEVTREQIEKDIFEHVISKVVPVEFLDENTNYYINPTGRFV
ECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCEE
VGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEVQLAYAIGIAKPVSISVETFGTGKIDDRKIVEIINKVFDLRPSAIIKELNLRRPIY
HHHEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
KQTAAYGHFGRTDVELPWESLNKLEEIKNLI
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLFTSESVTEGHPDKICDQISDAVLDAILERDPNGRVACETSVTTGMVLVTGEITTNC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCE
YVDIPKIVRNTIEAIGYTRAKYGFDSDTCAVLTSISEQSPDIAMGVDESLESKQGGMDKE
EEECHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCHH
DVIGAGDQGMMFGYANNETSEYMPMAISIAHKLSRRLSEVRKNGTLPYLRPDGKTQVTVE
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEE
YEDNKAIRIDTIVVSTQHGPEVTREQIEKDIFEHVISKVVPVEFLDENTNYYINPTGRFV
ECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCEE
VGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVA
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DKLEVQLAYAIGIAKPVSISVETFGTGKIDDRKIVEIINKVFDLRPSAIIKELNLRRPIY
HHHEEEEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHH
KQTAAYGHFGRTDVELPWESLNKLEEIKNLI
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129