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The map label for this gene is rho [H]

Identifier: 28210058

GI number: 28210058

Start: 279777

End: 281276

Strand: Direct

Name: rho [H]

Synonym: CTC00296

Alternate gene names: 28210058

Gene position: 279777-281276 (Clockwise)

Preceding gene: 28210057

Following gene: 28210059

Centisome position: 9.99

GC content: 30.2

Gene sequence:

>1500_bases
ATGTTTAAATTTTTTTGGAGGTGTTATTTTTTGTCTAGTAATGATTTAGATAAATTAACGGTAAATGAACTTAAAGAGAT
AGCTAAAGACCTAGAAATAAAAGGTGTTTATAAGTATAAAAAAAATGAGTTAATAGAGGAAATCAAAAAAGTTTCCCCTA
TGCATATAGAGAAAAATGGAGTTGTATTAAGAGAGAAGATAGTTCCAAAGAGTGGGAAGAGTAAACCTGAAGGAATTAAT
ATTGAGGAGAACAGTAACAGAGTGGAAAGTTTTCAAGGAGATAAAATAGAAGATAGAAGAAAAGAACAGGATAACAATAA
ACAAGAGGAAAATAAGAGAGAAAAATTAAGAGAAATGATAAATGAATCAGATACCGCAAAAGGTGTTCTTGAAATTATTG
AAAATAATAATTATGGTTTTTTAAGAGGTAAAAATTACTTAACAGGACCGGATGACATATATGTATCACCATCTCAAATA
AGAAGATTTAATTTGCAAACAGGAGATGAAGTTGAAGGTAAAGTTAGAACACCAAAAGAAGGAGAAAAATTTAAAGCACT
TCTTTATGTACAAAAAGTAAATGGAGAAAATCCAGAGAAGGCTGTTAGAAGACGTCCTTTTGAAAATCTTACCCCAATAT
ACCCTCAAGAAAGAATAAAATTAGAAAATAGTTCATCTGACCTATCTTCTAGACTAATGGATATAATATCCCCTATAGGT
AAAGGCCAAAGAGGAATAGTTGTTGCTCCTCCAAAGGCTGGGAAAACTACACTTCTTAAAAAGATCGCCCAAAGTATAAG
TATAAATCACCCAGATATGAAGTTAATAGTTTTACTAATAGATGAAAGACCAGAAGAAGTTACGGATATGCAAAGATCTA
TAAATGGTGAAGTGATATATTCAACTTTTGACGAAGAACCTGAGCACCATACAAAAGTAGCATATATGGTACTTGAGAGA
GCTAAAAGAATGGTAGAACAAGGTCAAAATGTTGTTATACTATTGGATAGTTTAACAAGACTTGCAAGAGCATATAATCT
TACAATAAATCCAACAGGAAGAACTTTATCAGGAGGATTAGATCCAGGTGCTTTAATAATGCCTAAAAAATTTTTTGGAG
CAGCTAGAAATATAGAAGAAGGAGGTAGCCTTACAATATTAGCTACTGCCTTAGTTGAAACTGGAAGCAGAATGGATGAT
ATGATATTTGAAGAATTTAAGGGAACGGGCAATATGGAAGTTCACTTAAATAGAAAACTGCAAGAAAGAAGAATATTCCC
AGCTATAGATATATACAGATCAGGAACTAGAAAAGAAAATTTATTATTATCAGAAAAAGAATATGAAGCATCATTTAATA
TAAGAAGATTACTTTATGATGAAAATAATACTCAAAATGTTACAGAACAACTTATAAATATGCTTTGTAAAACTAAGAAT
AATGATGAATTTGTAGAAGTTATAAGTAAAATTGACTTAAGTAAGGAAAAAAATAGATAG

Upstream 100 bases:

>100_bases
ATAAAACGTGTAGATTTTACTATGAAAATACTGTATAATTTAATAAATAATTAATCCTTTCAGATTATCTTTTAAGATTT
CCCAAGTTTTATAAAAAGAG

Downstream 100 bases:

>100_bases
GCGATTAAATTTAAATGAGCGGAATAATCTGCTCATTTAAATTATATTATTTTAAAACCATAGGGATAAATGATATATAA
ACAGTATCTAAAATGGGGGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MFKFFWRCYFLSSNDLDKLTVNELKEIAKDLEIKGVYKYKKNELIEEIKKVSPMHIEKNGVVLREKIVPKSGKSKPEGIN
IEENSNRVESFQGDKIEDRRKEQDNNKQEENKREKLREMINESDTAKGVLEIIENNNYGFLRGKNYLTGPDDIYVSPSQI
RRFNLQTGDEVEGKVRTPKEGEKFKALLYVQKVNGENPEKAVRRRPFENLTPIYPQERIKLENSSSDLSSRLMDIISPIG
KGQRGIVVAPPKAGKTTLLKKIAQSISINHPDMKLIVLLIDERPEEVTDMQRSINGEVIYSTFDEEPEHHTKVAYMVLER
AKRMVEQGQNVVILLDSLTRLARAYNLTINPTGRTLSGGLDPGALIMPKKFFGAARNIEEGGSLTILATALVETGSRMDD
MIFEEFKGTGNMEVHLNRKLQERRIFPAIDIYRSGTRKENLLLSEKEYEASFNIRRLLYDENNTQNVTEQLINMLCKTKN
NDEFVEVISKIDLSKEKNR

Sequences:

>Translated_499_residues
MFKFFWRCYFLSSNDLDKLTVNELKEIAKDLEIKGVYKYKKNELIEEIKKVSPMHIEKNGVVLREKIVPKSGKSKPEGIN
IEENSNRVESFQGDKIEDRRKEQDNNKQEENKREKLREMINESDTAKGVLEIIENNNYGFLRGKNYLTGPDDIYVSPSQI
RRFNLQTGDEVEGKVRTPKEGEKFKALLYVQKVNGENPEKAVRRRPFENLTPIYPQERIKLENSSSDLSSRLMDIISPIG
KGQRGIVVAPPKAGKTTLLKKIAQSISINHPDMKLIVLLIDERPEEVTDMQRSINGEVIYSTFDEEPEHHTKVAYMVLER
AKRMVEQGQNVVILLDSLTRLARAYNLTINPTGRTLSGGLDPGALIMPKKFFGAARNIEEGGSLTILATALVETGSRMDD
MIFEEFKGTGNMEVHLNRKLQERRIFPAIDIYRSGTRKENLLLSEKEYEASFNIRRLLYDENNTQNVTEQLINMLCKTKN
NDEFVEVISKIDLSKEKNR
>Mature_499_residues
MFKFFWRCYFLSSNDLDKLTVNELKEIAKDLEIKGVYKYKKNELIEEIKKVSPMHIEKNGVVLREKIVPKSGKSKPEGIN
IEENSNRVESFQGDKIEDRRKEQDNNKQEENKREKLREMINESDTAKGVLEIIENNNYGFLRGKNYLTGPDDIYVSPSQI
RRFNLQTGDEVEGKVRTPKEGEKFKALLYVQKVNGENPEKAVRRRPFENLTPIYPQERIKLENSSSDLSSRLMDIISPIG
KGQRGIVVAPPKAGKTTLLKKIAQSISINHPDMKLIVLLIDERPEEVTDMQRSINGEVIYSTFDEEPEHHTKVAYMVLER
AKRMVEQGQNVVILLDSLTRLARAYNLTINPTGRTLSGGLDPGALIMPKKFFGAARNIEEGGSLTILATALVETGSRMDD
MIFEEFKGTGNMEVHLNRKLQERRIFPAIDIYRSGTRKENLLLSEKEYEASFNIRRLLYDENNTQNVTEQLINMLCKTKN
NDEFVEVISKIDLSKEKNR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=368, Percent_Identity=58.4239130434783, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1788251, Length=284, Percent_Identity=27.4647887323944, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 57336; Mature: 57336

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFFWRCYFLSSNDLDKLTVNELKEIAKDLEIKGVYKYKKNELIEEIKKVSPMHIEKNG
CCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCEECCCC
VVLREKIVPKSGKSKPEGINIEENSNRVESFQGDKIEDRRKEQDNNKQEENKREKLREMI
EEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
NESDTAKGVLEIIENNNYGFLRGKNYLTGPDDIYVSPSQIRRFNLQTGDEVEGKVRTPKE
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECHHHHHEECCCCCCCCCCCCCCCCC
GEKFKALLYVQKVNGENPEKAVRRRPFENLTPIYPQERIKLENSSSDLSSRLMDIISPIG
CCCEEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHHC
KGQRGIVVAPPKAGKTTLLKKIAQSISINHPDMKLIVLLIDERPEEVTDMQRSINGEVIY
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
STFDEEPEHHTKVAYMVLERAKRMVEQGQNVVILLDSLTRLARAYNLTINPTGRTLSGGL
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCCCCCCC
DPGALIMPKKFFGAARNIEEGGSLTILATALVETGSRMDDMIFEEFKGTGNMEVHLNRKL
CCCCEECCHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCHH
QERRIFPAIDIYRSGTRKENLLLSEKEYEASFNIRRLLYDENNTQNVTEQLINMLCKTKN
HHCCCCCHHHHHHCCCCCCCEEECCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
NDEFVEVISKIDLSKEKNR
CHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFKFFWRCYFLSSNDLDKLTVNELKEIAKDLEIKGVYKYKKNELIEEIKKVSPMHIEKNG
CCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCEECCCC
VVLREKIVPKSGKSKPEGINIEENSNRVESFQGDKIEDRRKEQDNNKQEENKREKLREMI
EEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHH
NESDTAKGVLEIIENNNYGFLRGKNYLTGPDDIYVSPSQIRRFNLQTGDEVEGKVRTPKE
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECHHHHHEECCCCCCCCCCCCCCCCC
GEKFKALLYVQKVNGENPEKAVRRRPFENLTPIYPQERIKLENSSSDLSSRLMDIISPIG
CCCEEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHHC
KGQRGIVVAPPKAGKTTLLKKIAQSISINHPDMKLIVLLIDERPEEVTDMQRSINGEVIY
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEE
STFDEEPEHHTKVAYMVLERAKRMVEQGQNVVILLDSLTRLARAYNLTINPTGRTLSGGL
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCCCCCCC
DPGALIMPKKFFGAARNIEEGGSLTILATALVETGSRMDDMIFEEFKGTGNMEVHLNRKL
CCCCEECCHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCHH
QERRIFPAIDIYRSGTRKENLLLSEKEYEASFNIRRLLYDENNTQNVTEQLINMLCKTKN
HHCCCCCHHHHHHCCCCCCCEEECCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
NDEFVEVISKIDLSKEKNR
CHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]