Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 77747699

GI number: 77747699

Start: 1738698

End: 1739636

Strand: Reverse

Name: holB [H]

Synonym: PD1498

Alternate gene names: 77747699

Gene position: 1739636-1738698 (Counterclockwise)

Preceding gene: 28199379

Following gene: 28199377

Centisome position: 69.04

GC content: 55.48

Gene sequence:

>939_bases
ATGATGTCGGCACTTACCCCTTGGCAGCAGCGTGCTTACGATCAGATGGTGGCGGCGTTGGATGCTGGCCACCTTGGGCA
TGGCTTATTACTCTGCGGTCCTGAAGGATTAGGGCAACGTGCGGTTTCGCTTGCTTTTGCTGATACTGTCCTTGGCAAGG
GACTGACAGAAGTTGCACGCCAGCGTTGTCGTCAATTGATCACTGCCGGTACCCATCCTGATCTCCATTTGGTCTCGTTT
ATTCCGACGAAGACGGCAGACAAGCTGCGTACCGAAATCGTGATCGAACAGATACGTGACCTCTCTGAGAAGCTGGTGTT
GACTCCGCAGTATGGTCAAGCTCAAGTGGTGATCATTGATCCTGCTGATGCGATCAACCCCGCTGCGTGTAATGCATTGC
TGAAGACGCTTGAGGAGCCAGCCTCGGGGTGCTATCTCTGGTTGATCAGCCCACAGCCGGCACGACTGCCAGTGACCATT
CGCAGTCGTTGCCAGCGTTTAGAATTTAAATTGCCGCCGCTGCACGAAGCGCTGGCCTGGTTAGTGGCACAGGGCATCAC
TGAGAAGGTTGCCGTTGAAGCACTTGATGCGGCACGTGGCCATCCTGGTTTGGCTATGCAGTGGTTGTGCGATGAGGGGA
TGGCATTGCGCCGCAGGGTTGCTGTTGAGTTGGAACAGCTTGCCAGCGGCCAAATGAGTGCAGTTAAGTTAGCGCAGCAG
TGGACTGCTGACGGCAAGGCCGAACAGCGCCTGTATCATGCGGCTGATCTGGCCTTCATGCAGGCTACGTCTGTCTTGAC
CGATCCGTCACGTTTGCACAAGCTGGCGACTTGGTTCGACGCAGTCAACCGTACCCGTGATTTGCTCCGTACCACAGTAC
GTGCCGATCTGGCAGTCATTGAATTGTTGTTAGCTTGGCGCACCCGCTGGGAATGCTGA

Upstream 100 bases:

>100_bases
ATAACGCGGCGGTGGCGCGTTACTTGCAACGCCTTCGTGAACGCGAAAGCAAAACGAAGGAGCGGCAATGAATGGTGTTG
ATTGCCTAGGAAATTCATGG

Downstream 100 bases:

>100_bases
GCAGAATGTAAGGAGTATTGATGAGTACAACAAGTACGCGCCAGGGTATTCTGTCGCTCACCCTGAAAGACAGGGCGGCG
CTCTATAGCGCCTATATGAT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MMSALTPWQQRAYDQMVAALDAGHLGHGLLLCGPEGLGQRAVSLAFADTVLGKGLTEVARQRCRQLITAGTHPDLHLVSF
IPTKTADKLRTEIVIEQIRDLSEKLVLTPQYGQAQVVIIDPADAINPAACNALLKTLEEPASGCYLWLISPQPARLPVTI
RSRCQRLEFKLPPLHEALAWLVAQGITEKVAVEALDAARGHPGLAMQWLCDEGMALRRRVAVELEQLASGQMSAVKLAQQ
WTADGKAEQRLYHAADLAFMQATSVLTDPSRLHKLATWFDAVNRTRDLLRTTVRADLAVIELLLAWRTRWEC

Sequences:

>Translated_312_residues
MMSALTPWQQRAYDQMVAALDAGHLGHGLLLCGPEGLGQRAVSLAFADTVLGKGLTEVARQRCRQLITAGTHPDLHLVSF
IPTKTADKLRTEIVIEQIRDLSEKLVLTPQYGQAQVVIIDPADAINPAACNALLKTLEEPASGCYLWLISPQPARLPVTI
RSRCQRLEFKLPPLHEALAWLVAQGITEKVAVEALDAARGHPGLAMQWLCDEGMALRRRVAVELEQLASGQMSAVKLAQQ
WTADGKAEQRLYHAADLAFMQATSVLTDPSRLHKLATWFDAVNRTRDLLRTTVRADLAVIELLLAWRTRWEC
>Mature_312_residues
MMSALTPWQQRAYDQMVAALDAGHLGHGLLLCGPEGLGQRAVSLAFADTVLGKGLTEVARQRCRQLITAGTHPDLHLVSF
IPTKTADKLRTEIVIEQIRDLSEKLVLTPQYGQAQVVIIDPADAINPAACNALLKTLEEPASGCYLWLISPQPARLPVTI
RSRCQRLEFKLPPLHEALAWLVAQGITEKVAVEALDAARGHPGLAMQWLCDEGMALRRRVAVELEQLASGQMSAVKLAQQ
WTADGKAEQRLYHAADLAFMQATSVLTDPSRLHKLATWFDAVNRTRDLLRTTVRADLAVIELLLAWRTRWEC

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=204, Percent_Identity=32.843137254902, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786676, Length=171, Percent_Identity=29.2397660818713, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34458; Mature: 34458

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSALTPWQQRAYDQMVAALDAGHLGHGLLLCGPEGLGQRAVSLAFADTVLGKGLTEVAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
QRCRQLITAGTHPDLHLVSFIPTKTADKLRTEIVIEQIRDLSEKLVLTPQYGQAQVVIID
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEC
PADAINPAACNALLKTLEEPASGCYLWLISPQPARLPVTIRSRCQRLEFKLPPLHEALAW
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LVAQGITEKVAVEALDAARGHPGLAMQWLCDEGMALRRRVAVELEQLASGQMSAVKLAQQ
HHHHCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
WTADGKAEQRLYHAADLAFMQATSVLTDPSRLHKLATWFDAVNRTRDLLRTTVRADLAVI
HCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLLAWRTRWEC
HHHHHHHHCCCC
>Mature Secondary Structure
MMSALTPWQQRAYDQMVAALDAGHLGHGLLLCGPEGLGQRAVSLAFADTVLGKGLTEVAR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHH
QRCRQLITAGTHPDLHLVSFIPTKTADKLRTEIVIEQIRDLSEKLVLTPQYGQAQVVIID
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEC
PADAINPAACNALLKTLEEPASGCYLWLISPQPARLPVTIRSRCQRLEFKLPPLHEALAW
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LVAQGITEKVAVEALDAARGHPGLAMQWLCDEGMALRRRVAVELEQLASGQMSAVKLAQQ
HHHHCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
WTADGKAEQRLYHAADLAFMQATSVLTDPSRLHKLATWFDAVNRTRDLLRTTVRADLAVI
HCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLLAWRTRWEC
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]