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The map label for this gene is thiC

Identifier: 77747670

GI number: 77747670

Start: 1112871

End: 1114682

Strand: Reverse

Name: thiC

Synonym: PD0903

Alternate gene names: 77747670

Gene position: 1114682-1112871 (Counterclockwise)

Preceding gene: 28198808

Following gene: 28198804

Centisome position: 44.24

GC content: 51.32

Gene sequence:

>1812_bases
GTGAACATCACCACTGCCCTCCCCCTTCCCACCCACTTGCTGTCGGAGAAGGTGACTGCACCTTTACTTGGCGCACGCAA
AATTTACGTTACCGGATCACGCCAAGATATACGTGTGCCAATGCGCGAAATCGCACTCACACCCAGCTCAGCTCGATACG
GCGGAAACGAGAATCTATCACTGGTGCTCTATGACACTTCGGGAATTTACACTGATCCGCAAGCTACGATTGACTTAGCG
TGTGGTCTACCTCGATTACGCACTGCTTGGATTGATGAGCGTGCCGATACCGTAGAAGCTAAGTTGCATTTCAAAGTACC
TGAGTCGGTATCTGTCACCGCACCGCCTTTTCCTACACCGCCACAGCCACTGCGTGCACGTGACAATGTCGCGGTAACGC
AACTGGAATATGCACGACGTGGTCTCGTCACTCCAGAAATGGAGTTTGTCGCTATCCGTGAGCAACAACGCCGTGAACAA
ACTGTCGAAAATCTTCGCGGCCAGCGCCATGCGGGTGATGCCTGGGGGGCTTTGGTTGGCACACCCATCACTCCTGAATT
CGTACGTGATGAAATCGCGCGTGGCCGGGCCATTCTTCCTAATAACATCAACCATCCTGAAAGCGAGCCAATGATTATTG
GCCGCAACTTTTTGACAAAAATCAATGCCAATATTGGAACATCAGCCCTGTCTTCCAGTATTGCCGAAGAAGTGGAGAAG
CTGGTTTGGGCGATCCGCTGGGGCGCAGACACAATCATGGACCTCTCTACTGGCCGTGACATCCATGCTACTAGAGAATG
GATTCTGCGTAATTCACCTGTTCCTGTGGGGACGGTGCCAATTTATCAAGCGTTGGAGAAAGTCGATGGACATGTTGATG
CTCTGAGTTGGGACGTGTTTCGTGACACTTTGATCGAACAGGCCGAACAAGGTGTGGACTATGTCACCGTGCATGCAGGA
GTCCTTCGAGACTTTATCCCACTGACTGCTTCACGTTTAACAGGAATCGTCTCGCGTGGCGGTTCCATCATGGCTAGATG
GTGTCAGGCACACCGCAGCGAAAACTTCCTATACACGCATTTCGAGGAACTCTGTGAAATCATGCGTGCCTACGACGTGG
CATTCTCTCTGGGTGATGGATTGCGCCCAGGCTGCATCGCTGATGCGAATGATGCAGCTCAATTTGCTGAATTGGAGATT
CTTGGCAAGTTGACCCACATCGCCTGGAATCACCAAGTACAGGTGATGGTAGAAGGTCCTGGACACGTCCCAATGCAGCT
AATCAAGGCCAATATGGATAAGCAATTAGCCGCCTGTGGTGAGGCACCTTTCTACACGCTAGGTCCATTAACTACAGATA
TCGCCCCAGGTTACGACCACATTACTAGTGCAATTGGCGCGGCAATGATCGGCTGGTATGGGACAGCGATGCTATGTTAC
GTGACTCCCAAGGAGCACCTTGGCCTGCCTAATTTGCAAGATGTCCATGATGGCATCATCGCCTACAAAATTGCGGCACA
TGCGGCTGATCTTGCCAAAGGGCATCCAGCGGCACAGGCACGTGACGATGCACTCTCTAAAGCACGCTTTGAATTCCGCT
GGCAAGATCAATTCCATCTCAGCTTGGATCCAGAAAAAGCACTGGCACTGCATGATGAGAGCCTCCCTAAAGAAGCGCAT
AAACGAGCAGCATTCTGTTCGATGTGCGGACCGCAATTTTGTTCAATGAAAATCTCTCAAGAGGTCCGTGACGCTTCCTC
CAGCGAATTGTCAGATGCAAATACGGATATCAATACCAGCGACGGCATCTAG

Upstream 100 bases:

>100_bases
CCGGCGTAGGAAAGCTTCGTGGACCGCGCCGCATGGCCACCCTGGCAGGCGTAAGGTGCCGCGCCGCCGCTTCGTCCCGT
CACTCTCTGGACGAACCATC

Downstream 100 bases:

>100_bases
CATTCACTGTGTCTTTGGCATCGGAACGAATGATCTTTTCCGGTGTCAATCACTTTATGTTCTAGATTGACTCTCATTGA
TGTTCATTACACTAAAATTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLSLVLYDTSGIYTDPQATIDLA
CGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQ
TVENLRGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGTSALSSSIAEEVEK
LVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVFRDTLIEQAEQGVDYVTVHAG
VLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEI
LGKLTHIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCY
VTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHLSLDPEKALALHDESLPKEAH
KRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTSDGI

Sequences:

>Translated_603_residues
MNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLSLVLYDTSGIYTDPQATIDLA
CGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQ
TVENLRGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGTSALSSSIAEEVEK
LVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVFRDTLIEQAEQGVDYVTVHAG
VLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEI
LGKLTHIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCY
VTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHLSLDPEKALALHDESLPKEAH
KRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTSDGI
>Mature_603_residues
MNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLSLVLYDTSGIYTDPQATIDLA
CGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTPPQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQ
TVENLRGQRHAGDAWGALVGTPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGTSALSSSIAEEVEK
LVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVFRDTLIEQAEQGVDYVTVHAG
VLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTHFEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEI
LGKLTHIAWNHQVQVMVEGPGHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCY
VTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHLSLDPEKALALHDESLPKEAH
KRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTSDGI

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=575, Percent_Identity=64.8695652173913, Blast_Score=777, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_XYLFT (Q87CY6)

Other databases:

- EMBL:   AE009442
- RefSeq:   NP_779119.2
- STRING:   Q87CY6
- GeneID:   1143625
- GenomeReviews:   AE009442_GR
- KEGG:   xft:PD0903
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   XFAS183190:PD_0903-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 66343; Mature: 66343

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 224-224 BINDING 253-253 BINDING 282-282 BINDING 318-318 BINDING 418-418 BINDING 445-445

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLS
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCCCCCCCCCEE
LVLYDTSGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTP
EEEEECCCCEECCCCCEEHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCEECCCCCCCC
PQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQTVENLRGQRHAGDAWGALVG
CCCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
TPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGTSALSSSIAEEVEK
CCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
LVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVF
HHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
RDTLIEQAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTH
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
FEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEILGKLTHIAWNHQVQVMVEGP
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCEEEEEEECC
GHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCY
CCCHHHHHHHCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEE
VTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHL
ECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCEEE
SLDPEKALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTS
EECHHHHHEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DGI
CCC
>Mature Secondary Structure
MNITTALPLPTHLLSEKVTAPLLGARKIYVTGSRQDIRVPMREIALTPSSARYGGNENLS
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCCCCCCCCCEE
LVLYDTSGIYTDPQATIDLACGLPRLRTAWIDERADTVEAKLHFKVPESVSVTAPPFPTP
EEEEECCCCEECCCCCEEHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCEECCCCCCCC
PQPLRARDNVAVTQLEYARRGLVTPEMEFVAIREQQRREQTVENLRGQRHAGDAWGALVG
CCCCCCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
TPITPEFVRDEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGTSALSSSIAEEVEK
CCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
LVWAIRWGADTIMDLSTGRDIHATREWILRNSPVPVGTVPIYQALEKVDGHVDALSWDVF
HHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
RDTLIEQAEQGVDYVTVHAGVLRDFIPLTASRLTGIVSRGGSIMARWCQAHRSENFLYTH
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
FEELCEIMRAYDVAFSLGDGLRPGCIADANDAAQFAELEILGKLTHIAWNHQVQVMVEGP
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCEEEEEEECC
GHVPMQLIKANMDKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCY
CCCHHHHHHHCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEE
VTPKEHLGLPNLQDVHDGIIAYKIAAHAADLAKGHPAAQARDDALSKARFEFRWQDQFHL
ECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCEEE
SLDPEKALALHDESLPKEAHKRAAFCSMCGPQFCSMKISQEVRDASSSELSDANTDINTS
EECHHHHHEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DGI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12533478