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The map label for this gene is lysS [H]

Identifier: 28199805

GI number: 28199805

Start: 2278195

End: 2279148

Strand: Reverse

Name: lysS [H]

Synonym: PD1938

Alternate gene names: 28199805

Gene position: 2279148-2278195 (Counterclockwise)

Preceding gene: 28199806

Following gene: 28199804

Centisome position: 90.45

GC content: 57.13

Gene sequence:

>954_bases
TTGATGACCCTCATCGACACCTTGCGGCTGCGCGCCCGTTTAAACACCATGATCCGGCGCTTCTTTGCAGATCGCGGCGT
CTTAGAAGTTGAAACACCCCTCCTCTCCGCCGCCGGAAATACCGAGCCAAACATCCACAGCTTCACCACCTGCTTTACTG
GGCATTGTGATGCCGGAGCAGCACAACGTTGGCTGCGTACTTCGCCGGAATACCCCATGAAACGTCTCCTTGCTGCTGGT
ATCGGCGACTGCTACGAACTGGGGCGCGTCTTCCGTAACGGAGAAGCGGGCGGTCGCCATAACCCCGAATTCACCATGCT
CGAGTGGTATCGCGTCGGCTGGGATCACCACCGCCTGGCTGAGGAAACCACCGCCTTGGTCCGTGAAGCGCTGTCTCTTG
TCGAACGCACAGCTACATTGCATGTCACCACGTACCGTCAACTGTTCCTTGACATCCTTGACATTGATCCCCTACGTGCT
CCGCAAACCGTACTGCGTCAAGCCTTGGACGGTGGCGAGATCAACCCCGATGGATTGAGCCGTGATGATTGGCTTGACCT
ACTGATGACGCATCGCATCCAGCCGACATTCCCTACCGACCGCATCACCGTCATTGACGACTGGCCAGCCACACAGTGTG
CCTTGGCGCGGATCCGTTCCGATGACCCCCCGGTGGCGGAGCGCTTTGAACTCTACTTAGGTGTTCACGAGATCGCCAAC
GGTTACCATGAATTGAACGATGCAACTGAACAGCGCCGTCGTTTTGAACACGATAATCAAGTCCGTGCCGCACGCGGATT
GCCGCAGATGCCTCTTGACGAACCTCTCTTGAACGTCTTGTCCCGTCTTCCTGATTGCGCCGGTGTCGCCGTCGGTGTGG
ATCGATTACTGATGGCCATGAGCGCAACCGCATCCATTGCCGACGTCCTGGCGTTTTCCTTTATCTCTGCTTAG

Upstream 100 bases:

>100_bases
AAGTGAACCACCATAACAACGCGGCCAAGCGCTTCTACCTAGCATTAGGCTACCGCGATGAACACCGTGACCTGCTCAGC
CGTCGTTTGAACGAAACATC

Downstream 100 bases:

>100_bases
CCTGCGTTGCATGTCTGTTGGTGGGTTGATACCGTCCAACATGTGTACCAGCGCTGCTCTGTCGACTGTGGATGGGCGAG
TGCCGTTTCACTTAGACGTT

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MMTLIDTLRLRARLNTMIRRFFADRGVLEVETPLLSAAGNTEPNIHSFTTCFTGHCDAGAAQRWLRTSPEYPMKRLLAAG
IGDCYELGRVFRNGEAGGRHNPEFTMLEWYRVGWDHHRLAEETTALVREALSLVERTATLHVTTYRQLFLDILDIDPLRA
PQTVLRQALDGGEINPDGLSRDDWLDLLMTHRIQPTFPTDRITVIDDWPATQCALARIRSDDPPVAERFELYLGVHEIAN
GYHELNDATEQRRRFEHDNQVRAARGLPQMPLDEPLLNVLSRLPDCAGVAVGVDRLLMAMSATASIADVLAFSFISA

Sequences:

>Translated_317_residues
MMTLIDTLRLRARLNTMIRRFFADRGVLEVETPLLSAAGNTEPNIHSFTTCFTGHCDAGAAQRWLRTSPEYPMKRLLAAG
IGDCYELGRVFRNGEAGGRHNPEFTMLEWYRVGWDHHRLAEETTALVREALSLVERTATLHVTTYRQLFLDILDIDPLRA
PQTVLRQALDGGEINPDGLSRDDWLDLLMTHRIQPTFPTDRITVIDDWPATQCALARIRSDDPPVAERFELYLGVHEIAN
GYHELNDATEQRRRFEHDNQVRAARGLPQMPLDEPLLNVLSRLPDCAGVAVGVDRLLMAMSATASIADVLAFSFISA
>Mature_317_residues
MMTLIDTLRLRARLNTMIRRFFADRGVLEVETPLLSAAGNTEPNIHSFTTCFTGHCDAGAAQRWLRTSPEYPMKRLLAAG
IGDCYELGRVFRNGEAGGRHNPEFTMLEWYRVGWDHHRLAEETTALVREALSLVERTATLHVTTYRQLFLDILDIDPLRA
PQTVLRQALDGGEINPDGLSRDDWLDLLMTHRIQPTFPTDRITVIDDWPATQCALARIRSDDPPVAERFELYLGVHEIAN
GYHELNDATEQRRRFEHDNQVRAARGLPQMPLDEPLLNVLSRLPDCAGVAVGVDRLLMAMSATASIADVLAFSFISA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=335, Percent_Identity=30.1492537313433, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194272210, Length=331, Percent_Identity=29.9093655589124, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI87082379, Length=313, Percent_Identity=47.6038338658147, Blast_Score=270, Evalue=7e-74,
Organism=Escherichia coli, GI1790571, Length=332, Percent_Identity=32.2289156626506, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789256, Length=332, Percent_Identity=30.421686746988, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=29.2035398230088, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=29.2035398230088, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=339, Percent_Identity=29.2035398230088, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=341, Percent_Identity=28.7390029325513, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324256, Length=360, Percent_Identity=27.2222222222222, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24640849, Length=343, Percent_Identity=27.6967930029155, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640851, Length=343, Percent_Identity=27.6967930029155, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 35717; Mature: 35717

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLIDTLRLRARLNTMIRRFFADRGVLEVETPLLSAAGNTEPNIHSFTTCFTGHCDAGA
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCCHHHHHHHCCCCCCHH
AQRWLRTSPEYPMKRLLAAGIGDCYELGRVFRNGEAGGRHNPEFTMLEWYRVGWDHHRLA
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHCCHHHHHH
EETTALVREALSLVERTATLHVTTYRQLFLDILDIDPLRAPQTVLRQALDGGEINPDGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCC
RDDWLDLLMTHRIQPTFPTDRITVIDDWPATQCALARIRSDDPPVAERFELYLGVHEIAN
HHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GYHELNDATEQRRRFEHDNQVRAARGLPQMPLDEPLLNVLSRLPDCAGVAVGVDRLLMAM
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SATASIADVLAFSFISA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTLIDTLRLRARLNTMIRRFFADRGVLEVETPLLSAAGNTEPNIHSFTTCFTGHCDAGA
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCCCHHHHHHHCCCCCCHH
AQRWLRTSPEYPMKRLLAAGIGDCYELGRVFRNGEAGGRHNPEFTMLEWYRVGWDHHRLA
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHCCHHHHHH
EETTALVREALSLVERTATLHVTTYRQLFLDILDIDPLRAPQTVLRQALDGGEINPDGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCC
RDDWLDLLMTHRIQPTFPTDRITVIDDWPATQCALARIRSDDPPVAERFELYLGVHEIAN
HHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GYHELNDATEQRRRFEHDNQVRAARGLPQMPLDEPLLNVLSRLPDCAGVAVGVDRLLMAM
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
SATASIADVLAFSFISA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA