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The map label for this gene is amiC [H]

Identifier: 28199766

GI number: 28199766

Start: 2225782

End: 2227404

Strand: Reverse

Name: amiC [H]

Synonym: PD1898

Alternate gene names: 28199766

Gene position: 2227404-2225782 (Counterclockwise)

Preceding gene: 28199767

Following gene: 28199765

Centisome position: 88.4

GC content: 56.87

Gene sequence:

>1623_bases
ATGCTTGAATCATTCCCCATGATTCCGGTGAGTCTTCGATTGCGATACGTTGCCTTGCTGCTTGTGGCTAGTTTGGCGGT
GCTGGTGCCGCGGCTGCATGCGGGTGAGGTCAATGCGATCGAGTTGACCAGCAGCGCCAATGGAACCCGTGCCGAAATTC
GTCTTTCTGGTCGTGGTGCTTTCAAGACAATTTCGTTGTCTGCGCCAGAGCGTTTGGTGGTTGATTTCCCTGCTTCCAAT
GCCATTGAATCCCTGAAGTTGCCTACAGGGCGTGGTGTGGTGCGTAGTGTGCGTACTGGCCAGCCGGTGTCTGGAACGTT
TCGTGTGGTCTTTGACTTAGCGAATCCTGTCGCTCCGCTCAAACCGCAGATGCAAGTGATTGGCAATGTCTCCACGTTGG
TGATTGAGTGGCCCGGCGGTGTCAGTCACACATCCACTGCGGCGGTTGTGCCGTCTGGGAGCCGTCTGGGAGCAGAGCCG
GACCCTGTGCCGACGGTGATTGATTCACATGCCGAGGCCGCGCGTGCTACGGCGATGTTGACGGGCAAGGGACAACATAC
CTCGGCGATGCCGGTTGCTCTTGGATTGGGTGAGCTGACCACGCATGGGGTGTCACCTGCTGACATTCTCAACGGAGCAT
CGGTAGACGATGCTCGATCTAGGCCGACATCTGCTGCTGTCGCTGCACCTGTGTCTGCGCTTGCATCCTCTGCTGCGACG
GGAGCTGTTAACCCGTTGCCTGGCGGGACCGTTTCGCCGGTTGCGGATGTGAAAACTACCCCGGCAGAAGACACCGCATT
GGTGACGACGGCTCCCGTAGTGCCTGGCAATCTGTCGCGTATGCAGATGGCACCTGGTATGCGCTCCTTGGTCGTGGCGA
TTGATCCCGGTCACGGTGGCCAGGATTCTGGTGCGGTTGGGCCTACCGGTAAACTTGAGAAAAACGTGACGTTGGCGATT
GGGCGTGAGCTGGCGCGTCAGATCAATGCTACTCCCGGGATGAAGGCTTACATGACGCGTGATAGCGATGTGTTTATTCC
ACTGCCCATGCGGGCGCAAAGAGCACGTGCGGCCAAGGCTGATATTTTTATTTCGATCCATGCCGATGCGGCTGATAATC
GTGCTGCCACTGGTTCATCGGTCTATGTGCTGTCGACGAGGGGTGCTTCCTCACAGCGTGCGCGTTGGTTGGCGGATAAG
GAAAACGCCGCCGATTTGGTCGGTGGGGTACGTTTGCAAAAGGCCGAGCCAACGTTGGCTAACGTGTTATTGGATTTGGC
GCAGAGTGGTTATATGAAAGCCTCTGAAGATGCTGCCGATCATGTGCTGGGCAGCTTGAAGCGTGTCGCCAATAACCATA
AGTCGGAGGTGGAACACGCGAATTTTGCGGTGCTGCGTACCTCAGATATGCCAGCGATGCTTGTCGAGACTGCTTTCATT
TCCAATGCATATGAGGAACGGCGCTTGGTTGATCCCGCCTTCCAGCGCCAATTAGCTGCTGCAGTGTTGGAGGGAGTAAT
CACCTTTTTCACCAATCAGCCGCCTCCCGGTACGTTGTTCGCCTTGCGCGCACAAGCCGAGCCAGCCACGACTGGTGGCT
CGGTGATCACCAGCACGCATTAA

Upstream 100 bases:

>100_bases
ATAAAAATGCAATTGCCGGTTGTTTTTCCAGGAGTGCCCTAGGAAATTACTCCAGGCTATGGATTATTAAAGATAAATTT
CTTGCAATTCATAGGGGGCG

Downstream 100 bases:

>100_bases
GTTGCTTGATGGTGATTTGTCGATTCTTAGAGTGAATGTCCGGGTGTCGTTGCTGCGTGTTTCCCGTGTGTCTGAGTGTT
TGATCGTTTTAGATCTGATG

Product: N-acetylmuramoyl-L-alanine amidase precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLESFPMIPVSLRLRYVALLLVASLAVLVPRLHAGEVNAIELTSSANGTRAEIRLSGRGAFKTISLSAPERLVVDFPASN
AIESLKLPTGRGVVRSVRTGQPVSGTFRVVFDLANPVAPLKPQMQVIGNVSTLVIEWPGGVSHTSTAAVVPSGSRLGAEP
DPVPTVIDSHAEAARATAMLTGKGQHTSAMPVALGLGELTTHGVSPADILNGASVDDARSRPTSAAVAAPVSALASSAAT
GAVNPLPGGTVSPVADVKTTPAEDTALVTTAPVVPGNLSRMQMAPGMRSLVVAIDPGHGGQDSGAVGPTGKLEKNVTLAI
GRELARQINATPGMKAYMTRDSDVFIPLPMRAQRARAAKADIFISIHADAADNRAATGSSVYVLSTRGASSQRARWLADK
ENAADLVGGVRLQKAEPTLANVLLDLAQSGYMKASEDAADHVLGSLKRVANNHKSEVEHANFAVLRTSDMPAMLVETAFI
SNAYEERRLVDPAFQRQLAAAVLEGVITFFTNQPPPGTLFALRAQAEPATTGGSVITSTH

Sequences:

>Translated_540_residues
MLESFPMIPVSLRLRYVALLLVASLAVLVPRLHAGEVNAIELTSSANGTRAEIRLSGRGAFKTISLSAPERLVVDFPASN
AIESLKLPTGRGVVRSVRTGQPVSGTFRVVFDLANPVAPLKPQMQVIGNVSTLVIEWPGGVSHTSTAAVVPSGSRLGAEP
DPVPTVIDSHAEAARATAMLTGKGQHTSAMPVALGLGELTTHGVSPADILNGASVDDARSRPTSAAVAAPVSALASSAAT
GAVNPLPGGTVSPVADVKTTPAEDTALVTTAPVVPGNLSRMQMAPGMRSLVVAIDPGHGGQDSGAVGPTGKLEKNVTLAI
GRELARQINATPGMKAYMTRDSDVFIPLPMRAQRARAAKADIFISIHADAADNRAATGSSVYVLSTRGASSQRARWLADK
ENAADLVGGVRLQKAEPTLANVLLDLAQSGYMKASEDAADHVLGSLKRVANNHKSEVEHANFAVLRTSDMPAMLVETAFI
SNAYEERRLVDPAFQRQLAAAVLEGVITFFTNQPPPGTLFALRAQAEPATTGGSVITSTH
>Mature_540_residues
MLESFPMIPVSLRLRYVALLLVASLAVLVPRLHAGEVNAIELTSSANGTRAEIRLSGRGAFKTISLSAPERLVVDFPASN
AIESLKLPTGRGVVRSVRTGQPVSGTFRVVFDLANPVAPLKPQMQVIGNVSTLVIEWPGGVSHTSTAAVVPSGSRLGAEP
DPVPTVIDSHAEAARATAMLTGKGQHTSAMPVALGLGELTTHGVSPADILNGASVDDARSRPTSAAVAAPVSALASSAAT
GAVNPLPGGTVSPVADVKTTPAEDTALVTTAPVVPGNLSRMQMAPGMRSLVVAIDPGHGGQDSGAVGPTGKLEKNVTLAI
GRELARQINATPGMKAYMTRDSDVFIPLPMRAQRARAAKADIFISIHADAADNRAATGSSVYVLSTRGASSQRARWLADK
ENAADLVGGVRLQKAEPTLANVLLDLAQSGYMKASEDAADHVLGSLKRVANNHKSEVEHANFAVLRTSDMPAMLVETAFI
SNAYEERRLVDPAFQRQLAAAVLEGVITFFTNQPPPGTLFALRAQAEPATTGGSVITSTH

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=45.3333333333333, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=44.5414847161572, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=45, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 56030; Mature: 56030

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESFPMIPVSLRLRYVALLLVASLAVLVPRLHAGEVNAIELTSSANGTRAEIRLSGRGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
FKTISLSAPERLVVDFPASNAIESLKLPTGRGVVRSVRTGQPVSGTFRVVFDLANPVAPL
EEEEEECCCCEEEEECCCCCCHHHCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCC
KPQMQVIGNVSTLVIEWPGGVSHTSTAAVVPSGSRLGAEPDPVPTVIDSHAEAARATAML
CCHHHHHCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHCHHHHHHHEEEE
TGKGQHTSAMPVALGLGELTTHGVSPADILNGASVDDARSRPTSAAVAAPVSALASSAAT
ECCCCCCCCCEEEECCCHHHHCCCCHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCC
GAVNPLPGGTVSPVADVKTTPAEDTALVTTAPVVPGNLSRMQMAPGMRSLVVAIDPGHGG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCC
QDSGAVGPTGKLEKNVTLAIGRELARQINATPGMKAYMTRDSDVFIPLPMRAQRARAAKA
CCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHCCCC
DIFISIHADAADNRAATGSSVYVLSTRGASSQRARWLADKENAADLVGGVRLQKAEPTLA
EEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHHHCCEEEECCCCHHH
NVLLDLAQSGYMKASEDAADHVLGSLKRVANNHKSEVEHANFAVLRTSDMPAMLVETAFI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCHHHHHHHHH
SNAYEERRLVDPAFQRQLAAAVLEGVITFFTNQPPPGTLFALRAQAEPATTGGSVITSTH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECC
>Mature Secondary Structure
MLESFPMIPVSLRLRYVALLLVASLAVLVPRLHAGEVNAIELTSSANGTRAEIRLSGRGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCCC
FKTISLSAPERLVVDFPASNAIESLKLPTGRGVVRSVRTGQPVSGTFRVVFDLANPVAPL
EEEEEECCCCEEEEECCCCCCHHHCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCC
KPQMQVIGNVSTLVIEWPGGVSHTSTAAVVPSGSRLGAEPDPVPTVIDSHAEAARATAML
CCHHHHHCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHCHHHHHHHEEEE
TGKGQHTSAMPVALGLGELTTHGVSPADILNGASVDDARSRPTSAAVAAPVSALASSAAT
ECCCCCCCCCEEEECCCHHHHCCCCHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCC
GAVNPLPGGTVSPVADVKTTPAEDTALVTTAPVVPGNLSRMQMAPGMRSLVVAIDPGHGG
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCC
QDSGAVGPTGKLEKNVTLAIGRELARQINATPGMKAYMTRDSDVFIPLPMRAQRARAAKA
CCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHCCCC
DIFISIHADAADNRAATGSSVYVLSTRGASSQRARWLADKENAADLVGGVRLQKAEPTLA
EEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHHHCCEEEECCCCHHH
NVLLDLAQSGYMKASEDAADHVLGSLKRVANNHKSEVEHANFAVLRTSDMPAMLVETAFI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCHHHHHHHHH
SNAYEERRLVDPAFQRQLAAAVLEGVITFFTNQPPPGTLFALRAQAEPATTGGSVITSTH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]