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The map label for this gene is trmB

Identifier: 28199745

GI number: 28199745

Start: 2206273

End: 2207004

Strand: Reverse

Name: trmB

Synonym: PD1876

Alternate gene names: 28199745

Gene position: 2207004-2206273 (Counterclockwise)

Preceding gene: 28199746

Following gene: 28199744

Centisome position: 87.59

GC content: 51.91

Gene sequence:

>732_bases
ATGAATCTGCTCAGCAGCGATGGTGTCCAAGTGTTGCCTAGACCCTTCACCCTCAATGAAAGACGCCGTGAAGTGCGTAG
TTTTGTATTGCGTCAGGGGCATTTTACGCCTGCTCAGAAGCGCGCTTTTGATCATTATTGGCCGCGCTTTGGTGTGGATT
TTATCGGTCAGTTGCGTGATCTGGATGTTTTGTTCGGACGCAGTGCTCCGAAGGTGCTGGAGGTTGGTTTCGGTAATGGT
GCTGCACTGCGTTTTGCTGCACAGCATGAGCCTAGATATGACTATATCGGTATTGAAGTGTATGCGCCGGGTGTAGGACG
TTTGCTCAACGGATTGGCTGAGGATGGGAGTCGTCATGTGCGTCTGTATCACTACGATGCAGTCGAAGTGTTGAATAAGG
AAATCGTTGATGGTGCGCTTGATGAGATTCGCATCTATTTTCCTGATCCATGGCACAAGAAGCGCCATCATAAGCGTCGT
TTGATTCAGCCGTTATTCGCTACGCTACTCGTACGTAAGTTGCGTGTTGGCGGATGTTTGCACATGGCGACGGATTGGGC
TGATTATGCTGAGCAGATGTGGGATGTGCTTGATGCCACGCCAGGATTGGTCAATCGCGCTGGCCTGCGTGGCCAAGTGC
CTTGCCCCGATTGGCGAGTTCAGACGCGCTTTGAGAGGCGTGGCCAAAATCTGGGCCACAGAGTCTGGAATCTGCTCTAC
GATCGGGTCTGA

Upstream 100 bases:

>100_bases
GTAAGGCTGTTGAGGCTGGACGTGAAGCGTTCCTGGCGGGACGTATCCCACGGAAGCGTTACGCTGCGGCATCCAGTCCA
GTGGAGGGATTGGTTGGATG

Downstream 100 bases:

>100_bases
ATTCTGCCACTGGGATCACTACATCATCACTCCATGGATACTGCTTTGACGTTGACCAATGACATGAAGCTGGTGCTCGG
TTTGGTCGGTTTTACGATGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNLLSSDGVQVLPRPFTLNERRREVRSFVLRQGHFTPAQKRAFDHYWPRFGVDFIGQLRDLDVLFGRSAPKVLEVGFGNG
AALRFAAQHEPRYDYIGIEVYAPGVGRLLNGLAEDGSRHVRLYHYDAVEVLNKEIVDGALDEIRIYFPDPWHKKRHHKRR
LIQPLFATLLVRKLRVGGCLHMATDWADYAEQMWDVLDATPGLVNRAGLRGQVPCPDWRVQTRFERRGQNLGHRVWNLLY
DRV

Sequences:

>Translated_243_residues
MNLLSSDGVQVLPRPFTLNERRREVRSFVLRQGHFTPAQKRAFDHYWPRFGVDFIGQLRDLDVLFGRSAPKVLEVGFGNG
AALRFAAQHEPRYDYIGIEVYAPGVGRLLNGLAEDGSRHVRLYHYDAVEVLNKEIVDGALDEIRIYFPDPWHKKRHHKRR
LIQPLFATLLVRKLRVGGCLHMATDWADYAEQMWDVLDATPGLVNRAGLRGQVPCPDWRVQTRFERRGQNLGHRVWNLLY
DRV
>Mature_243_residues
MNLLSSDGVQVLPRPFTLNERRREVRSFVLRQGHFTPAQKRAFDHYWPRFGVDFIGQLRDLDVLFGRSAPKVLEVGFGNG
AALRFAAQHEPRYDYIGIEVYAPGVGRLLNGLAEDGSRHVRLYHYDAVEVLNKEIVDGALDEIRIYFPDPWHKKRHHKRR
LIQPLFATLLVRKLRVGGCLHMATDWADYAEQMWDVLDATPGLVNRAGLRGQVPCPDWRVQTRFERRGQNLGHRVWNLLY
DRV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=221, Percent_Identity=48.4162895927602, Blast_Score=221, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_XYLF2 (B2I9C6)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830655.1
- ProteinModelPortal:   B2I9C6
- GeneID:   6202751
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1982
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28192; Mature: 28192

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: BINDING 75-75 BINDING 100-100 BINDING 127-127 BINDING 150-150 BINDING 154-154 BINDING 186-186

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLSSDGVQVLPRPFTLNERRREVRSFVLRQGHFTPAQKRAFDHYWPRFGVDFIGQLRD
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
LDVLFGRSAPKVLEVGFGNGAALRFAAQHEPRYDYIGIEVYAPGVGRLLNGLAEDGSRHV
HHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEE
RLYHYDAVEVLNKEIVDGALDEIRIYFPDPWHKKRHHKRRLIQPLFATLLVRKLRVGGCL
EEEEHHHHHHHHHHHHHCCHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
HMATDWADYAEQMWDVLDATPGLVNRAGLRGQVPCPDWRVQTRFERRGQNLGHRVWNLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
DRV
HCC
>Mature Secondary Structure
MNLLSSDGVQVLPRPFTLNERRREVRSFVLRQGHFTPAQKRAFDHYWPRFGVDFIGQLRD
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
LDVLFGRSAPKVLEVGFGNGAALRFAAQHEPRYDYIGIEVYAPGVGRLLNGLAEDGSRHV
HHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEE
RLYHYDAVEVLNKEIVDGALDEIRIYFPDPWHKKRHHKRRLIQPLFATLLVRKLRVGGCL
EEEEHHHHHHHHHHHHHCCHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
HMATDWADYAEQMWDVLDATPGLVNRAGLRGQVPCPDWRVQTRFERRGQNLGHRVWNLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
DRV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA