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The map label for this gene is mraW [H]

Identifier: 28199742

GI number: 28199742

Start: 2201582

End: 2202550

Strand: Reverse

Name: mraW [H]

Synonym: PD1873

Alternate gene names: 28199742

Gene position: 2202550-2201582 (Counterclockwise)

Preceding gene: 28199743

Following gene: 28199741

Centisome position: 87.41

GC content: 55.42

Gene sequence:

>969_bases
TTGGATGGCTTGGCACCGCACTTGTCAATGACTCATGTCCCAGTGCTCTACACCCAGGCCATGGAAGGGTTGCGGGTGGT
TGAAAACGGAACCTATTTGGATGGCACATTCGGGCGTGGAGGACACGCACGAGGGGTGCTGCAACAACTAGGCCCTGGAG
GCCGACTGCTAGTGATGGATAAGGATCCAGAAGCGATCGCAATGGCTGAACGTGCGTTCAGCTGTGATCCGCGCGTGGTC
ATTCGTCATGGCAGCTTTGCTTTGCTCGCGCAGTTGGCTGCGCCACAGTCTTTGGATGGTGTGTTGTTTGATCTAGGAGT
GTCTTCGCCGCAGTTGGATGTTCCAGAGCGTGGTTTTTCTTTTGCTAAGGATGGCCCGCTTGATATGCGCATGGATCCGG
AGATGGGGGAGAGTGCGGCGCAGTGGCTGGCACGTGTCTCCGAGCGTGAAATCGCTGACGTACTTTGGACCTACGGCGAG
GAAAAACAGAGCCGTCGTATTGCGCGTGCGATTGTCGCCTACCGCGCCAATCAGCCGTTGTTACGTACCGTGCAGTTGGC
TGAATTAATTGCATCTGTGATGTTACGCACTAAGTTTGGTGCCTGTAAATCCAGGATTCATCCTGCGACGCGCAGTTTTC
AGGGAATCCGTATCCATGTGAACCGTGAGTTGGTGGATTTGGAGGTTGGTCTTGAAGCAGCACTCGCAGCATTGCGCCCG
GGTGGGCGGTTGGTCGTGATCAGTTTTCATTCACTGGAGGACCGTATCGTCAAACAGTTTATCAGTCGTCATTCCAAGGT
GCCGCCTACCAATCGTCGCCTACCTGAGGTGCAGACCTTCGTGCCGTTATTACGGATGATCGGTCGTGCGATCAAGGCCG
ATGAGGATGAGTTGGAGGTTAACCCGCGTGCGCGCAGTGCGGTGCTTCGAGTGGCTGAGAAGTTGGATGTGTTGGGGGCG
GTGCGATGA

Upstream 100 bases:

>100_bases
CATCACGCATTGATCCAGCAGACACTCTCTGATGGTGATTTGGGCGATGGACTACTCGATCTCAGATTGTGAGTGGGGAC
GGATGTGTGACGAGGGGTGC

Downstream 100 bases:

>100_bases
GCCGTCTGCTGTTGATTATTCTTTTGGCTTGTACGGTCGCTTCGGCGATCGGTGTGGTTTACATGCGCCATCTGCATCGA
AAGTTATTCGTTGAGCTGTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMDKDPEAIAMAERAFSCDPRVV
IRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGE
EKQSRRIARAIVAYRANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAALAALRP
GGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEVNPRARSAVLRVAEKLDVLGA
VR

Sequences:

>Translated_322_residues
MDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMDKDPEAIAMAERAFSCDPRVV
IRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGE
EKQSRRIARAIVAYRANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAALAALRP
GGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEVNPRARSAVLRVAEKLDVLGA
VR
>Mature_322_residues
MDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMDKDPEAIAMAERAFSCDPRVV
IRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGE
EKQSRRIARAIVAYRANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAALAALRP
GGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEVNPRARSAVLRVAEKLDVLGA
VR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=39.6449704142012, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=42.1052631578947, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=50.7836990595611, Blast_Score=291, Evalue=3e-80,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=39.7014925373134, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=39.7014925373134, Blast_Score=184, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35518; Mature: 35518

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMD
CCCCCCCCCHHHHHHHHHHHHCCCEEECCCCEECCCCCCCCHHHHHHHHCCCCCEEEEEE
KDPEAIAMAERAFSCDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFS
CCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCC
FAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGEEKQSRRIARAIVAYRANQPL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
LRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAALAALRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCEEEEHHHHHHHHHHHHCC
GGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEV
CCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEC
NPRARSAVLRVAEKLDVLGAVR
CCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMD
CCCCCCCCCHHHHHHHHHHHHCCCEEECCCCEECCCCCCCCHHHHHHHHCCCCCEEEEEE
KDPEAIAMAERAFSCDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFS
CCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCC
FAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGEEKQSRRIARAIVAYRANQPL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
LRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAALAALRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCEEEEHHHHHHHHHHHHCC
GGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEV
CCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEC
NPRARSAVLRVAEKLDVLGAVR
CCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA