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The map label for this gene is ddl [H]

Identifier: 28199733

GI number: 28199733

Start: 2190697

End: 2191611

Strand: Reverse

Name: ddl [H]

Synonym: PD1864

Alternate gene names: 28199733

Gene position: 2191611-2190697 (Counterclockwise)

Preceding gene: 28199734

Following gene: 28199732

Centisome position: 86.98

GC content: 52.57

Gene sequence:

>915_bases
GTGTTTGGTCGTGTTGCAGTGTTATTAGGGGGGACGTCTGCTGAGCGTGAGGTGTCTTTGCTTTCAGGTCGTAATGTGTT
GGAAGTATTGCGCATGCGCGGTGTTGATGCTCAGTCGGTAGATGGGGTTCCGGCACTCGCTCAGGCGCTGGTGGAACGGC
GTTTTGATCGTGTGTTCAATGTGTTGCACGGTCATAATGGCGGTGGTGAGGATGGTGTGGTGCAGGGGTTGATGCAGGCT
TTTGGTGTGCCGTATACAGGTTCGGATGTGCTTGGTTCAGCGTTGAGTATGGATAAGGTGCGCACTAAACAGGTTTGGCT
TGCGCTGGGTTTGCCGACGCCGCGTTATGCGAGTTTGAGTGTGTGTGCCACTGCTGTTGAGGTGCGTAAGGCGGTTGAGA
TGCTAGGTTTTCCGGTGATTATTAAGCCGGCTAAAGAAGGGTCTAGTGTTGGTGTCAGTCGTGTGTTTGCACTGGAGCAT
TTGGAGGAGGCGGTTGCTTTAGCGGCGCGTTACGAGGGTGAGTTGCTGATGGAGCAGTTGATCGAAGGGGATGAATTGAC
GGTGTCGATTCTTGATGAAATGGCGTTGCCCTCAATTCGGATCGTTCCTCAGGGGCAGTGGTATGACTATAACGCGAAGT
ATCTTGCTGAAGACACGCAGTATGTGTGCCCGGGTTTGGATGATGTTGCTGAGGCTGAAATCGCTCAACTTGCGTTGGCT
GCATTCCATAGTGTTGGTTGTCGTGGTTGGGGTCGTGTGGATGTGATGCGCGAGCGCGGCAGTGGGCGCTTTTTCTTATT
GGAAGTCAATACTGCTCCTGGAATGACGACTCACTCGTTAGTGCCAAAGGCAGCGAGTCAGTTGGGTATGGGTTTTGATG
ATTTGGTGTGGCGGATTCTGGAGCAGACTTTATGA

Upstream 100 bases:

>100_bases
CCATCGCGCAGCGGATTGTTCATGATGGTTTTGTCTTTGGAGAGGTTTAAATATGGGTATGAGCATATTTTCACTGTCTC
CACGGTATACGGATCCGGCG

Downstream 100 bases:

>100_bases
GTTGCTTTGGTATCCGTATGGATGGGAGGCAGGACGGCTGTGCATTTGTTGCTGGAAGTACGGTAATGGTGGTGTCTTGT
GTATCGTCCGCTTGGAGTGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFNVLHGHNGGGEDGVVQGLMQA
FGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEH
LEEAVALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCPGLDDVAEAEIAQLALA
AFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRILEQTL

Sequences:

>Translated_304_residues
MFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFNVLHGHNGGGEDGVVQGLMQA
FGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEH
LEEAVALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCPGLDDVAEAEIAQLALA
AFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRILEQTL
>Mature_304_residues
MFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFNVLHGHNGGGEDGVVQGLMQA
FGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEH
LEEAVALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCPGLDDVAEAEIAQLALA
AFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRILEQTL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=310, Percent_Identity=51.2903225806452, Blast_Score=290, Evalue=7e-80,
Organism=Escherichia coli, GI1786579, Length=354, Percent_Identity=30.7909604519774, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 32890; Mature: 32890

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFN
CCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLHGHNGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLS
HHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHH
VCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEHLEEAVALAARYEGELLMEQL
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCPGLDDVAEAEIAQLALA
HCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHH
AFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRIL
HHHHHCCCCCCHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
EQTL
HHCC
>Mature Secondary Structure
MFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFN
CCCEEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLHGHNGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLS
HHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHH
VCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEHLEEAVALAARYEGELLMEQL
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCPGLDDVAEAEIAQLALA
HCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHH
AFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRIL
HHHHHCCCCCCHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
EQTL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]