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The map label for this gene is engXCA [H]

Identifier: 28199725

GI number: 28199725

Start: 2178971

End: 2180680

Strand: Reverse

Name: engXCA [H]

Synonym: PD1856

Alternate gene names: 28199725

Gene position: 2180680-2178971 (Counterclockwise)

Preceding gene: 28199726

Following gene: 28199723

Centisome position: 86.54

GC content: 52.92

Gene sequence:

>1710_bases
GTGGCCTCCGCCATGTACGGTGGAACGGTGGGTGCACAAAATGCAGTGTCTGATACTCACTTTGTTGAACCACTCCGCGG
CGTTAACTGGCGTGGTTTGGAAACGGCGCAGCACCTGCCGCAAGGTTTAAATCAACGTCCCTGGCGTGAAGTACTCGACC
AAATGCAGAGCCTAGGCATTAACGCAATACGCCTGCCCCTGTGCTCGGACACCCTACATGGCGCCATGCCTACCAATCTG
GATCTAGTGCGTAATCCAGATCTCAAAGGACGTACCGCATTGCAGATTGCCGACGCAATCATGGATGAAGCTGGGAAACG
TGGGATGCGGGTCCTCCTGGCGTATCACGGCGTGGAGTGTCCTACCGACAGAAACCCATTATTGCGCAGCGTAGATGAAT
CAGAGCACCAATGGATCAGCGATGTGCAATTCATCACCTCGCATTATCGTGCTCAACAAAAAGTAGTGATGGGCGTGGAC
TTGGCCGACATGGCAAACCATCGCCCCTTTCAGAGCGGTGGTGATAGCGCGCCTGATTGGAATCCTGTCGTCGAGCGTGC
CGCCGCTGCCATTCTGGCGATGAATCCCGACTGGTTGATTGGTGTGCAGCTTGTTGGCCTGAATCCCCCCTGCTTGGATG
CCTCCGCTCCCATCTCCGATGACAACATAAAATCGCAGCACTGTGTCCAATTACGCATTCCTGCTCGGAGCCTCTTGCTC
ATGCCGCGCTTCGCGGGCACTGACATGGATACTGAAGCTGCCCTTGGCGCATTTTCTGGAAAACAAACCGTGTTACCCAA
CTCTCTGGATGCCACGGATGCCGAGCAGCTCGCCCATCGGATTGATGCACTGCTGGCATTTGGTATACGCCAAGGCTTTT
ATGGATCTTGGATGACCTCAGCACAGATGCCATTTGGCCTGCTCGATAACGACGGCCGTACCCCACGTACCTCATTGATT
GCACAACTACATCGTTGGTGGGGTGTTAGCCGTGTCGATGTTGCCAGTGAGAATGCTACGACGAAGAATCAAACAACGAC
CGATACCAATGGATGTGTTGCTGGTGATAACAGCGTGCCACTCAATGGTTGGGACACCTCTTTCAGTGGTGTCGCCACCT
ATACCTATACGGGTTACAAAGGCGGCGCATTGATGCTGGATCCGATTCAGTCCCATGTGCAAATCACTGCACTGAATCCT
ACTCAACTCAATTTGGGGGGGATTCCCGCTGCGATGGCCGGTGCTTATTTGCGTGTGCAGGGTCCGAAGGGAAGCACAAC
CGTCTACGTGACGGACCTCTATCCCACCGGATCTTCCGGGGGATTAGATCTTTCGCCGAATGCCTTTGCCAGTATCGGCA
ATATGGCTCAAGGGCGTATCCCCGTGCAATGGAAGGTCGTCTCAGCACCTGTGAGTGGCAACTTGATCTATCGGGTAAAA
AAGGGAAGCTCGGGATGGTGGGCAGCGATCCAAGTACGTGAACATCGGTATCCAGTCCTCAAACTTGAAATCTGTCAGGA
TGGCACCTGGTTGAATTTGCCAAAGAGAAATTACAACTACTTTGTTGGTACCCGACTGGGTAACCAACCCTTGTCAATGC
GCATGACAGATATTCGTGGTCAGACCTTGATTGATACGCTTCCCGCCTTGCCCAATAAGGCTTCCTCAAAAGCATATTCT
GTGAATGGAAATGTCCAGTTTTCCGAATAA

Upstream 100 bases:

>100_bases
TAGCTCTGCGCTATGGGGACAGTTGTGTGTTAGTCCAACACAAATGAGGTATTAGATGCGACGCTTGGCAATCTTATTTT
GGCTTGCAACATCCGGTTGC

Downstream 100 bases:

>100_bases
GACCGTGACTGGCTCAGCCTGGTTCTCCTACCTGAGTGGTGATTGCCTGAACAGATTAATGAGGTTTTTAAAACTGCTTT
AAGCGCACTGACCTTGGAGC

Product: extracellular endoglucanase precursor

Products: NA

Alternate protein names: EXLX1 [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MASAMYGGTVGAQNAVSDTHFVEPLRGVNWRGLETAQHLPQGLNQRPWREVLDQMQSLGINAIRLPLCSDTLHGAMPTNL
DLVRNPDLKGRTALQIADAIMDEAGKRGMRVLLAYHGVECPTDRNPLLRSVDESEHQWISDVQFITSHYRAQQKVVMGVD
LADMANHRPFQSGGDSAPDWNPVVERAAAAILAMNPDWLIGVQLVGLNPPCLDASAPISDDNIKSQHCVQLRIPARSLLL
MPRFAGTDMDTEAALGAFSGKQTVLPNSLDATDAEQLAHRIDALLAFGIRQGFYGSWMTSAQMPFGLLDNDGRTPRTSLI
AQLHRWWGVSRVDVASENATTKNQTTTDTNGCVAGDNSVPLNGWDTSFSGVATYTYTGYKGGALMLDPIQSHVQITALNP
TQLNLGGIPAAMAGAYLRVQGPKGSTTVYVTDLYPTGSSGGLDLSPNAFASIGNMAQGRIPVQWKVVSAPVSGNLIYRVK
KGSSGWWAAIQVREHRYPVLKLEICQDGTWLNLPKRNYNYFVGTRLGNQPLSMRMTDIRGQTLIDTLPALPNKASSKAYS
VNGNVQFSE

Sequences:

>Translated_569_residues
MASAMYGGTVGAQNAVSDTHFVEPLRGVNWRGLETAQHLPQGLNQRPWREVLDQMQSLGINAIRLPLCSDTLHGAMPTNL
DLVRNPDLKGRTALQIADAIMDEAGKRGMRVLLAYHGVECPTDRNPLLRSVDESEHQWISDVQFITSHYRAQQKVVMGVD
LADMANHRPFQSGGDSAPDWNPVVERAAAAILAMNPDWLIGVQLVGLNPPCLDASAPISDDNIKSQHCVQLRIPARSLLL
MPRFAGTDMDTEAALGAFSGKQTVLPNSLDATDAEQLAHRIDALLAFGIRQGFYGSWMTSAQMPFGLLDNDGRTPRTSLI
AQLHRWWGVSRVDVASENATTKNQTTTDTNGCVAGDNSVPLNGWDTSFSGVATYTYTGYKGGALMLDPIQSHVQITALNP
TQLNLGGIPAAMAGAYLRVQGPKGSTTVYVTDLYPTGSSGGLDLSPNAFASIGNMAQGRIPVQWKVVSAPVSGNLIYRVK
KGSSGWWAAIQVREHRYPVLKLEICQDGTWLNLPKRNYNYFVGTRLGNQPLSMRMTDIRGQTLIDTLPALPNKASSKAYS
VNGNVQFSE
>Mature_568_residues
ASAMYGGTVGAQNAVSDTHFVEPLRGVNWRGLETAQHLPQGLNQRPWREVLDQMQSLGINAIRLPLCSDTLHGAMPTNLD
LVRNPDLKGRTALQIADAIMDEAGKRGMRVLLAYHGVECPTDRNPLLRSVDESEHQWISDVQFITSHYRAQQKVVMGVDL
ADMANHRPFQSGGDSAPDWNPVVERAAAAILAMNPDWLIGVQLVGLNPPCLDASAPISDDNIKSQHCVQLRIPARSLLLM
PRFAGTDMDTEAALGAFSGKQTVLPNSLDATDAEQLAHRIDALLAFGIRQGFYGSWMTSAQMPFGLLDNDGRTPRTSLIA
QLHRWWGVSRVDVASENATTKNQTTTDTNGCVAGDNSVPLNGWDTSFSGVATYTYTGYKGGALMLDPIQSHVQITALNPT
QLNLGGIPAAMAGAYLRVQGPKGSTTVYVTDLYPTGSSGGLDLSPNAFASIGNMAQGRIPVQWKVVSAPVSGNLIYRVKK
GSSGWWAAIQVREHRYPVLKLEICQDGTWLNLPKRNYNYFVGTRLGNQPLSMRMTDIRGQTLIDTLPALPNKASSKAYSV
NGNVQFSE

Specific function: May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non- covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been

COG id: COG2730

COG function: function code G; Endoglucanase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 expansin-like EG45 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014733
- InterPro:   IPR009009
- InterPro:   IPR007117 [H]

Pfam domain/function: PF03330 DPBB_1 [H]

EC number: NA

Molecular weight: Translated: 61791; Mature: 61660

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAMYGGTVGAQNAVSDTHFVEPLRGVNWRGLETAQHLPQGLNQRPWREVLDQMQSLGI
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
NAIRLPLCSDTLHGAMPTNLDLVRNPDLKGRTALQIADAIMDEAGKRGMRVLLAYHGVEC
CEEEEEEECCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
PTDRNPLLRSVDESEHQWISDVQFITSHYRAQQKVVMGVDLADMANHRPFQSGGDSAPDW
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHCCCCCCCCCCCCCCC
NPVVERAAAAILAMNPDWLIGVQLVGLNPPCLDASAPISDDNIKSQHCVQLRIPARSLLL
CHHHHHHHHEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECHHHEEE
MPRFAGTDMDTEAALGAFSGKQTVLPNSLDATDAEQLAHRIDALLAFGIRQGFYGSWMTS
EECCCCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCC
AQMPFGLLDNDGRTPRTSLIAQLHRWWGVSRVDVASENATTKNQTTTDTNGCVAGDNSVP
CCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCC
LNGWDTSFSGVATYTYTGYKGGALMLDPIQSHVQITALNPTQLNLGGIPAAMAGAYLRVQ
CCCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEECCCEEECCCCCHHHCCCEEEEE
GPKGSTTVYVTDLYPTGSSGGLDLSPNAFASIGNMAQGRIPVQWKVVSAPVSGNLIYRVK
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEE
KGSSGWWAAIQVREHRYPVLKLEICQDGTWLNLPKRNYNYFVGTRLGNQPLSMRMTDIRG
ECCCCEEEEEEEECCCCCEEEEEEECCCCEEECCCCCCCEEEEEECCCCCCEEEEECCCC
QTLIDTLPALPNKASSKAYSVNGNVQFSE
CHHHHHHCCCCCCCCCCEEEECCCEEECC
>Mature Secondary Structure 
ASAMYGGTVGAQNAVSDTHFVEPLRGVNWRGLETAQHLPQGLNQRPWREVLDQMQSLGI
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCC
NAIRLPLCSDTLHGAMPTNLDLVRNPDLKGRTALQIADAIMDEAGKRGMRVLLAYHGVEC
CEEEEEEECCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
PTDRNPLLRSVDESEHQWISDVQFITSHYRAQQKVVMGVDLADMANHRPFQSGGDSAPDW
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHCCCCCCCCCCCCCCC
NPVVERAAAAILAMNPDWLIGVQLVGLNPPCLDASAPISDDNIKSQHCVQLRIPARSLLL
CHHHHHHHHEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECHHHEEE
MPRFAGTDMDTEAALGAFSGKQTVLPNSLDATDAEQLAHRIDALLAFGIRQGFYGSWMTS
EECCCCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCC
AQMPFGLLDNDGRTPRTSLIAQLHRWWGVSRVDVASENATTKNQTTTDTNGCVAGDNSVP
CCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCC
LNGWDTSFSGVATYTYTGYKGGALMLDPIQSHVQITALNPTQLNLGGIPAAMAGAYLRVQ
CCCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEECCCEEECCCCCHHHCCCEEEEE
GPKGSTTVYVTDLYPTGSSGGLDLSPNAFASIGNMAQGRIPVQWKVVSAPVSGNLIYRVK
CCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEE
KGSSGWWAAIQVREHRYPVLKLEICQDGTWLNLPKRNYNYFVGTRLGNQPLSMRMTDIRG
ECCCCEEEEEEEECCCCCEEEEEEECCCCEEECCCCCCCEEEEEECCCCCCEEEEECCCC
QTLIDTLPALPNKASSKAYSVNGNVQFSE
CHHHHHHCCCCCCCCCCEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]