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The map label for this gene is nspV [H]

Identifier: 28199486

GI number: 28199486

Start: 1859967

End: 1860629

Strand: Reverse

Name: nspV [H]

Synonym: PD1608

Alternate gene names: 28199486

Gene position: 1860629-1859967 (Counterclockwise)

Preceding gene: 28199487

Following gene: 28199485

Centisome position: 73.84

GC content: 57.92

Gene sequence:

>663_bases
ATGCAGCTGGACATTGAATCGCTCTGCCGCGAAGCACAGGAATTTTCGCGCATCGAATCCAGCCATCCCGAGCCGTCGCT
CTTCGGCGTCACCGACGGCAAGGCGGTCGGGACGTACCTCGAAGCCAAGTTCTGCGGGTACCTGCACGCGAAAGGCTACG
AGTTCGAGGCGGGCAATGCCGCCAACGGCTTGGACTTCCCTAGCTTGAACGTCGATATCAAGGTCACGAGCATTAAGCAG
CCGCAATCGTCTTGCCCATTCCGGTCCATCCGCCAGAAAATCTACGGGCTCGGGTACTGCCTACTCGTGTTCGTCTATGA
GAAGACGGACGACCCTACGACACGCACCGCGATCCTCGGCATGACGGATACCGTGTTCGTCGAATCAGAAGTGACCGCCG
ACTTCCAGATGACGAAGGGCTTGCTTGAAGTTCTTGATGCCGATGGCAATGAGGATGACCTGACCTCATTCATCATGGCA
AGGCATCTGACTACGGATGAGATAGAACTGCGACAGCTTGCTGAGGAGGTTATGGAGCGCCGCCCAGTGCAAGGCTACCT
GACCATTTCCCCCGCGCTGCAGTGGCGCCTACAGTACAAGCGCGTCATTCAGAAGGCTGGCGAAGTTGCCGGAGTGCGCT
CGATCTACAAGGCCGGACGGTGA

Upstream 100 bases:

>100_bases
CGCGGGTGCACCTGGCCAAGCAATCCACAGCATGTTTGTTGATCGGTATGGCGCCGTACCCGATAGTGTTACATTGACGT
TGAAAGTATCGGAGAACCGG

Downstream 100 bases:

>100_bases
ACGAAGCAAAGAAACGCAAAATTGAGTTCGGAGACTTCCAGACTCCGTCTGCACTAGCGCGCCTCGTTTGCGAAAGACTG
TCCTTGCTAGGCTTTGCGCC

Product: type II restriction enzyme NspV

Products: NA

Alternate protein names: R.NspV; Endonuclease NspV; Type II restriction enzyme NspV [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MQLDIESLCREAQEFSRIESSHPEPSLFGVTDGKAVGTYLEAKFCGYLHAKGYEFEAGNAANGLDFPSLNVDIKVTSIKQ
PQSSCPFRSIRQKIYGLGYCLLVFVYEKTDDPTTRTAILGMTDTVFVESEVTADFQMTKGLLEVLDADGNEDDLTSFIMA
RHLTTDEIELRQLAEEVMERRPVQGYLTISPALQWRLQYKRVIQKAGEVAGVRSIYKAGR

Sequences:

>Translated_220_residues
MQLDIESLCREAQEFSRIESSHPEPSLFGVTDGKAVGTYLEAKFCGYLHAKGYEFEAGNAANGLDFPSLNVDIKVTSIKQ
PQSSCPFRSIRQKIYGLGYCLLVFVYEKTDDPTTRTAILGMTDTVFVESEVTADFQMTKGLLEVLDADGNEDDLTSFIMA
RHLTTDEIELRQLAEEVMERRPVQGYLTISPALQWRLQYKRVIQKAGEVAGVRSIYKAGR
>Mature_220_residues
MQLDIESLCREAQEFSRIESSHPEPSLFGVTDGKAVGTYLEAKFCGYLHAKGYEFEAGNAANGLDFPSLNVDIKVTSIKQ
PQSSCPFRSIRQKIYGLGYCLLVFVYEKTDDPTTRTAILGMTDTVFVESEVTADFQMTKGLLEVLDADGNEDDLTSFIMA
RHLTTDEIELRQLAEEVMERRPVQGYLTISPALQWRLQYKRVIQKAGEVAGVRSIYKAGR

Specific function: Recognizes the double-stranded sequence TTCGAA and cleaves after T-2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 24664; Mature: 24664

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDIESLCREAQEFSRIESSHPEPSLFGVTDGKAVGTYLEAKFCGYLHAKGYEFEAGNA
CCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCCC
ANGLDFPSLNVDIKVTSIKQPQSSCPFRSIRQKIYGLGYCLLVFVYEKTDDPTTRTAILG
CCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHEEEEC
MTDTVFVESEVTADFQMTKGLLEVLDADGNEDDLTSFIMARHLTTDEIELRQLAEEVMER
CCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
RPVQGYLTISPALQWRLQYKRVIQKAGEVAGVRSIYKAGR
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLDIESLCREAQEFSRIESSHPEPSLFGVTDGKAVGTYLEAKFCGYLHAKGYEFEAGNA
CCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCCC
ANGLDFPSLNVDIKVTSIKQPQSSCPFRSIRQKIYGLGYCLLVFVYEKTDDPTTRTAILG
CCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHEEEEC
MTDTVFVESEVTADFQMTKGLLEVLDADGNEDDLTSFIMARHLTTDEIELRQLAEEVMER
CCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
RPVQGYLTISPALQWRLQYKRVIQKAGEVAGVRSIYKAGR
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8367311 [H]