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The map label for this gene is aroA [H]

Identifier: 28199240

GI number: 28199240

Start: 1576935

End: 1578299

Strand: Reverse

Name: aroA [H]

Synonym: PD1356

Alternate gene names: 28199240

Gene position: 1578299-1576935 (Counterclockwise)

Preceding gene: 28199241

Following gene: 28199239

Centisome position: 62.64

GC content: 54.36

Gene sequence:

>1365_bases
TTGTATTGTCGTCGCTCTCAGTTGAAGAAACCTAGTATGAGTCATAGAACGCATTACTATTGGATCGCACACCAGGGTAC
CCCACTGCATGGCGTCCTGAGTATCCCCGGCGATAAATCAATCTCTCATCGTGCAGTCATGTTTGCTGCCCTTGCGGATG
GCACGTCACGTATTGATGGCTTTCTTGAGGCGGAGGATACGCGCTCTACAGCAGCGATCTTGGCCCGATTGGGTGTGCGT
ATCGAAACTCCCTCATTCACGCAGCGCATCGTCCATGGTGTTGGTGTGGATGGACTCCAGGCATCGGATATTGCCCTGGA
TTGTGGCAATGCAGGCACTGGCATGCGCCTGCTCGCTGGTTTGCTGGTAGCGCAGCCTTTTGACAGCGTTTTAGTCGGAG
ATGCATCACTGTCCAAGCGACCGATGCGACGTGTGACGGATCCGCTGTCACAGATGGGCGCACGTATCGATACCAGCGAC
GATGGTACTCCACCGCTGCGTATTTACGGTGGTCAATTACTCCGCGGTATCGATTTTATCTCCCCAGTGGCCAGTGCTCA
GATCAAGTCAGCGGTGTTGCTGGCTGGATTGTATGCACGTAACGAAACGGTAGTGCGTGAACCGCACCCGACGCGTGATT
ACACCGAGCGTATGCTCACTGCGTTTGGTGTGGACATTGATGTTTCCACAGGGTGCGTGCGGTTGCGTGGTGGGCAACGG
TTATGTGCTACCAATATTACAATCCCGGCTGATTTTTCCTCAGCTGCGTTTTATCTGGTTGCAGCCAGCGTGATTCCTGG
CTCTGATATCACCCTGCGTGCTGTTGGACTCAATCCGCGTCGTATTGGTTTGTTAACTGTGTTGCGGCTGATGGGGGCAG
ATATTGTTGAATCCAATCGCCATGAACAGGGTGGTGAGCCGGTTGCTGACCTACGTGTGCGTTATGCGTCACTCCAGGGC
ACGCGTGTTCCTGAAGATTTGGTGCCGGATATGATTGACGAATTCCCTGCCTTGTTTGTCGCTGCAGCGGCAGCCGAAGG
TCAAACGGTAGTGAGTGGTGCGGCTGAACTACGCGTTAAAGAATCGGACCGGTTGGCTGCGATGGTGACAGGCTTGCGCG
TGCTTGGCGTTCAGGTGGATGAGACCGCCGACGGGGCAACGATTCATGGAGGGCCCATCGGTCATGGCACCATCAACAGC
CATGGCGATCATCGCATCGCCATGGCGTTTTCAATTGCAGGGCAGCTTTCCGTCAGTACAGTACGTATTGAAGATGTCGC
CAATGTTGCGACTTCTTTTCCAGACTATGAGACGTTAGCGCGCAGCGCTGGTTTCGGTCTTGAGGTGTACTGCGATCCAG
CATGA

Upstream 100 bases:

>100_bases
GCGGTGAAGGTCAAGATCCTCGGAGCCTATCCAATTGCCCTTCTATGATGCTTGAGTATTTCTGTCCAGGAGCGACACGC
ATTACCGCAGCCTCGGCGGT

Downstream 100 bases:

>100_bases
ATGAACGCCATTAATATCTGTACTGCCTTGTAAGCCATGCGATGGTTGTTCTCACTTAAAGAAGCCCAGCAGTTCTTTCG
TGTTTCTTTCACTTGTTCTT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MYCRRSQLKKPSMSHRTHYYWIAHQGTPLHGVLSIPGDKSISHRAVMFAALADGTSRIDGFLEAEDTRSTAAILARLGVR
IETPSFTQRIVHGVGVDGLQASDIALDCGNAGTGMRLLAGLLVAQPFDSVLVGDASLSKRPMRRVTDPLSQMGARIDTSD
DGTPPLRIYGGQLLRGIDFISPVASAQIKSAVLLAGLYARNETVVREPHPTRDYTERMLTAFGVDIDVSTGCVRLRGGQR
LCATNITIPADFSSAAFYLVAASVIPGSDITLRAVGLNPRRIGLLTVLRLMGADIVESNRHEQGGEPVADLRVRYASLQG
TRVPEDLVPDMIDEFPALFVAAAAAEGQTVVSGAAELRVKESDRLAAMVTGLRVLGVQVDETADGATIHGGPIGHGTINS
HGDHRIAMAFSIAGQLSVSTVRIEDVANVATSFPDYETLARSAGFGLEVYCDPA

Sequences:

>Translated_454_residues
MYCRRSQLKKPSMSHRTHYYWIAHQGTPLHGVLSIPGDKSISHRAVMFAALADGTSRIDGFLEAEDTRSTAAILARLGVR
IETPSFTQRIVHGVGVDGLQASDIALDCGNAGTGMRLLAGLLVAQPFDSVLVGDASLSKRPMRRVTDPLSQMGARIDTSD
DGTPPLRIYGGQLLRGIDFISPVASAQIKSAVLLAGLYARNETVVREPHPTRDYTERMLTAFGVDIDVSTGCVRLRGGQR
LCATNITIPADFSSAAFYLVAASVIPGSDITLRAVGLNPRRIGLLTVLRLMGADIVESNRHEQGGEPVADLRVRYASLQG
TRVPEDLVPDMIDEFPALFVAAAAAEGQTVVSGAAELRVKESDRLAAMVTGLRVLGVQVDETADGATIHGGPIGHGTINS
HGDHRIAMAFSIAGQLSVSTVRIEDVANVATSFPDYETLARSAGFGLEVYCDPA
>Mature_454_residues
MYCRRSQLKKPSMSHRTHYYWIAHQGTPLHGVLSIPGDKSISHRAVMFAALADGTSRIDGFLEAEDTRSTAAILARLGVR
IETPSFTQRIVHGVGVDGLQASDIALDCGNAGTGMRLLAGLLVAQPFDSVLVGDASLSKRPMRRVTDPLSQMGARIDTSD
DGTPPLRIYGGQLLRGIDFISPVASAQIKSAVLLAGLYARNETVVREPHPTRDYTERMLTAFGVDIDVSTGCVRLRGGQR
LCATNITIPADFSSAAFYLVAASVIPGSDITLRAVGLNPRRIGLLTVLRLMGADIVESNRHEQGGEPVADLRVRYASLQG
TRVPEDLVPDMIDEFPALFVAAAAAEGQTVVSGAAELRVKESDRLAAMVTGLRVLGVQVDETADGATIHGGPIGHGTINS
HGDHRIAMAFSIAGQLSVSTVRIEDVANVATSFPDYETLARSAGFGLEVYCDPA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=426, Percent_Identity=32.3943661971831, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320332, Length=438, Percent_Identity=28.5388127853881, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 48323; Mature: 48323

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYCRRSQLKKPSMSHRTHYYWIAHQGTPLHGVLSIPGDKSISHRAVMFAALADGTSRIDG
CCCCCHHCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHEEEEEEHHCCHHHHCC
FLEAEDTRSTAAILARLGVRIETPSFTQRIVHGVGVDGLQASDIALDCGNAGTGMRLLAG
EEECCCCHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHH
LLVAQPFDSVLVGDASLSKRPMRRVTDPLSQMGARIDTSDDGTPPLRIYGGQLLRGIDFI
HHHHCCCCCEEECCCHHCCCHHHHHHHHHHHHCCEECCCCCCCCCEEEECHHHHHHHHHH
SPVASAQIKSAVLLAGLYARNETVVREPHPTRDYTERMLTAFGVDIDVSTGCVRLRGGQR
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCEEECCCCEEEECCCCE
LCATNITIPADFSSAAFYLVAASVIPGSDITLRAVGLNPRRIGLLTVLRLMGADIVESNR
EEEECCEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHCCC
HEQGGEPVADLRVRYASLQGTRVPEDLVPDMIDEFPALFVAAAAAEGQTVVSGAAELRVK
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEC
ESDRLAAMVTGLRVLGVQVDETADGATIHGGPIGHGTINSHGDHRIAMAFSIAGQLSVST
CCCHHHHHHHHHHHEEEEEECCCCCCEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEE
VRIEDVANVATSFPDYETLARSAGFGLEVYCDPA
EEHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MYCRRSQLKKPSMSHRTHYYWIAHQGTPLHGVLSIPGDKSISHRAVMFAALADGTSRIDG
CCCCCHHCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHEEEEEEHHCCHHHHCC
FLEAEDTRSTAAILARLGVRIETPSFTQRIVHGVGVDGLQASDIALDCGNAGTGMRLLAG
EEECCCCHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHH
LLVAQPFDSVLVGDASLSKRPMRRVTDPLSQMGARIDTSDDGTPPLRIYGGQLLRGIDFI
HHHHCCCCCEEECCCHHCCCHHHHHHHHHHHHCCEECCCCCCCCCEEEECHHHHHHHHHH
SPVASAQIKSAVLLAGLYARNETVVREPHPTRDYTERMLTAFGVDIDVSTGCVRLRGGQR
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCEEECCCCEEEECCCCE
LCATNITIPADFSSAAFYLVAASVIPGSDITLRAVGLNPRRIGLLTVLRLMGADIVESNR
EEEECCEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHCCC
HEQGGEPVADLRVRYASLQGTRVPEDLVPDMIDEFPALFVAAAAAEGQTVVSGAAELRVK
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEC
ESDRLAAMVTGLRVLGVQVDETADGATIHGGPIGHGTINSHGDHRIAMAFSIAGQLSVST
CCCHHHHHHHHHHHEEEEEECCCCCCEECCCCCCCCCCCCCCCCEEEEEEEECCCEEEEE
VRIEDVANVATSFPDYETLARSAGFGLEVYCDPA
EEHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]