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The map label for this gene is xthA [H]

Identifier: 28198769

GI number: 28198769

Start: 1074703

End: 1075491

Strand: Reverse

Name: xthA [H]

Synonym: PD0864

Alternate gene names: 28198769

Gene position: 1075491-1074703 (Counterclockwise)

Preceding gene: 28198770

Following gene: 28198768

Centisome position: 42.68

GC content: 53.87

Gene sequence:

>789_bases
ATGCGTATTGCAAGTTGGAACGTTAATTCACTCAATGTACGTTTGTCTCAGTTGCAGCAGTGGCTGGTGGAGGCTGCGCC
TGATGTGCTTGGCCTTCAGGAAACGAAGTTGGAGGACCATCAGTTTCCAGAAGCAGCGTTGGCGGCATTGGGCTATGTCA
GTGTGTATGCGGGGCAGAAGACGTATAACGGTGTCGCACTTCTGGCACGCGTGGCGTTGGAGGATGTGCAAGTCGGTATC
CCGGAGTTCGAGGATGTGCAGCGACGGGTGATCGCTGCAACTGTGGATGGGATACGCGTTGTCAATCTATACGTGGTTAA
TGGTCAAGAGGTTGGGACAGACAAGTACGCTTACAAATTAAGCTGGTTAGATGCGGTGTACCACTGGCTGGCCGAGGAGC
TGCAACGCTATCCCCGCCTTGTGGTTCTGGGTGATTTCAATATTGCTCCTGATGCCCGGGACGTCTACGATCCGCAGGTA
TGGAATGAGGATCATATTCTCACTTCGACCACGGAACGTGCGGCACTGGATAAGCTGTTAGGACTGGGGTTGCATGATGG
TTTTCGCTTGCATCACCAGGATGCGGGGATATTCAGTTGGTGGGATTACCGGCAAGCTAGTTTCCGGCGCAATAGGGGCT
TACGTATTGATCTCACGCTTGTTTCGGAGGCGTTGCGCGCGCAGGCGGTGGCTTCTGGAATCGACCGCGTGCCTAGAGGC
TGGGAACGACCCAGTGATCATGCTCCAGCGTGGATTGAATTAGCTTTGGCGGTGTCTTCATCACCTTAG

Upstream 100 bases:

>100_bases
AGTAAGGGTTACTGCGCCTAGGGTTGTAGGTAGGCCAAGTAGATAAGAATTACCTGGGATGTGGGCTGTTTTTATATGAA
ATCAATTTTTTAATATGAAA

Downstream 100 bases:

>100_bases
GCGTTCGGTGTTGAGAGACCGCCCGAAAACGTCAATGGGAGGTGTGTTATCTCACGCAATAACGACCACGTTTTGATGTC
ATGCAAGTGCGGTAAGTACG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQKTYNGVALLARVALEDVQVGI
PEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQV
WNEDHILTSTTERAALDKLLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVPRG
WERPSDHAPAWIELALAVSSSP

Sequences:

>Translated_262_residues
MRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQKTYNGVALLARVALEDVQVGI
PEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQV
WNEDHILTSTTERAALDKLLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVPRG
WERPSDHAPAWIELALAVSSSP
>Mature_262_residues
MRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQKTYNGVALLARVALEDVQVGI
PEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQV
WNEDHILTSTTERAALDKLLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVPRG
WERPSDHAPAWIELALAVSSSP

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=32.1969696969697, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=32.1969696969697, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=32.1969696969697, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=39.0151515151515, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=28.8973384030418, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=30.6513409961686, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=30.6513409961686, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29483; Mature: 29483

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQK
CEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHEECCCC
TYNGVALLARVALEDVQVGIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHEEHH
SWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQVWNEDHILTSTTERAALDKLL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHH
GLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVPRG
HCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
WERPSDHAPAWIELALAVSSSP
CCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQK
CEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHEECCCC
TYNGVALLARVALEDVQVGIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHEEHH
SWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQVWNEDHILTSTTERAALDKLL
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHH
GLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVPRG
HCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
WERPSDHAPAWIELALAVSSSP
CCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]