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The map label for this gene is cysJ [H]

Identifier: 28198622

GI number: 28198622

Start: 878204

End: 880063

Strand: Reverse

Name: cysJ [H]

Synonym: PD0716

Alternate gene names: 28198622

Gene position: 880063-878204 (Counterclockwise)

Preceding gene: 28198625

Following gene: 77747655

Centisome position: 34.93

GC content: 55.43

Gene sequence:

>1860_bases
TTGCTCCGGCTCATCACAAGCATGACTGCCCCCACCTATTCCCTTACCGCAAGCCCCCTCCCCGAGGACCGTCGCTCCCT
GCTCATGGATCTCGTCAAGGGCCTGGACACACCGTCCCTATGGTGGCTATCCGGCTATGTCGCTGGACTGGCACAAAGCC
AACTCACATCCACATTAACAGCGGTACAAGAAGAAGATTCAGGGACCCAAGCCAAACAACGCCTCACCGTGCTCTACGGC
AGCCAGACCGGCAACGCACAGCGCATTGCTGAACAACTCGCCGCCGAAGCCGAAGCCGCAGGACTGGCAAGGCGCCTAAT
TAGAGCCGATGACTACACCCCTCGTGAACTAGCTGCAGAACGGCTGCTGTACATCGTCATCAGTACTCAGGGAGAAGGTG
ATCCACCAGATGACTCCATCGGCTTTGTGGAATTTCTGAATGAGCGACGCGCACCCAAATTACCCGAACTGAAATACGCC
GTCCTTGGCCTTGGAGACTCCAGTTACGCTGACTTCTGCGGCATCGCCCGCCGTCTGGACGCACGATTGCAAGCACTCGG
TGCACAACGGCTGCAACCATTAGGCGAAGCCGATGTGGATATCGATACAGTCGCCACGCCATGGCTGAAACAAGCGCTGC
ACCATGCCCGCGAGCAACTTAAAAACGCCACCGCCCCACTGACCAATGTCACACCGTTACGCGGCAATTCAGCTCTCCGT
GGATCCAATCAGACGTTCCAAGCTGAACTGTTCAAAAACCAACTGGTCAGCGGACGCGACTTTAAAGGTCCAGCGTTCCG
AACACATGGCCAGGCCTCGAAACGGGTCCACCACCTAGAGCTGTCGCTGGAAGGCAGCGGATTACACTACGAACCAGGCG
ACGCACTGGGCGTACATCACCGTAATCCCAATACGCTTGTAGATGCTGTCCTTACGACACTTAAACTTTCAGCTCACACC
GAAGTAACACAAGATAAGGAAACCTTGCCGCTGTCCGAGTGGCTCGCCACCCGACGCGAAGTGACCAGATTGTCGCGACC
ATTCTTGGCTGCGCATGCCGAACGATCCGGTGCTGAGGAACTGCGCGCGCTACTCACCCCAACCCAGACTGCCGGACTAG
CGACGCTACTCAACGATCATCAACTGATCGACGTATTGCGACGCTGGCCGGCGGATTGGGACCATACCGCACTGGTTGCA
GCATTACGCCCCCTCACACCACGGCTGTATTCGATTGCTTCCAGCCGCAAGCGTGTCGGCGAGGAAGTCCACATCACGAT
TGATGAACTGCACTATCAAGCACACGGCCACGAACACATTGGTGCAGCAAGCAGCTTTCTGGCTGGTTTGACAGAAGCAG
ATACCCTCCCTGTCTACATCCAACCCAATGAGCGCTTCCGTTTACCGGCGGATGCAACACGCGACATCATCATGATTGGC
TCTGGAACTGGAGTAGCACCATTCCGCGCTTTCGTCCAAGAACGCGCAGAAAGTGGCGCAACCGGGCGTAACTGGTTGTT
CTTCGGCGCACAACACTTCAATTGCGACTTTCTGTATCAGACCGAATGGCAACAAGCCTTACAACGCGGTGAACTGCACG
CATTGGATCTTGCATTCTCCCGTGACCACGCTGAAAAAATCTACGTACAGCACCGCTTATTAGCACAGGGGCAAGAGGTG
TACACCTGGCTCCAAAATGGCGCATATCTGTATGTCTGCGGCGCAATCAGCATGAGTAAAGACGTCCATACAACACTGCT
ACAAATCCTCGTCACACATGGCAACCTCACCAAAGAAGCCGCCGCAGAGACGTTGATTGGAATGCAACGCGAAGGACGCT
ATGCACGTGATGTGTATTAA

Upstream 100 bases:

>100_bases
GTGTAGCGAAATTAACCATGTTCTGAAATAAGCACGAAGCATAAGGGGATAACCTTCCTTCCTTTCCGCACCATTTCGCA
CCTCCCTAACATCTATTCGC

Downstream 100 bases:

>100_bases
TAGGCGCAACAACAGCCTGACTCGTATCGTGGCCATGCGTCGCTACAATCCCAACGACACATGGCGACCATGTCGCCCCA
AACATCCTTGTCCATCCTCC

Product: NADPH-sulfite reductase flavoprotein subunit

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MLRLITSMTAPTYSLTASPLPEDRRSLLMDLVKGLDTPSLWWLSGYVAGLAQSQLTSTLTAVQEEDSGTQAKQRLTVLYG
SQTGNAQRIAEQLAAEAEAAGLARRLIRADDYTPRELAAERLLYIVISTQGEGDPPDDSIGFVEFLNERRAPKLPELKYA
VLGLGDSSYADFCGIARRLDARLQALGAQRLQPLGEADVDIDTVATPWLKQALHHAREQLKNATAPLTNVTPLRGNSALR
GSNQTFQAELFKNQLVSGRDFKGPAFRTHGQASKRVHHLELSLEGSGLHYEPGDALGVHHRNPNTLVDAVLTTLKLSAHT
EVTQDKETLPLSEWLATRREVTRLSRPFLAAHAERSGAEELRALLTPTQTAGLATLLNDHQLIDVLRRWPADWDHTALVA
ALRPLTPRLYSIASSRKRVGEEVHITIDELHYQAHGHEHIGAASSFLAGLTEADTLPVYIQPNERFRLPADATRDIIMIG
SGTGVAPFRAFVQERAESGATGRNWLFFGAQHFNCDFLYQTEWQQALQRGELHALDLAFSRDHAEKIYVQHRLLAQGQEV
YTWLQNGAYLYVCGAISMSKDVHTTLLQILVTHGNLTKEAAAETLIGMQREGRYARDVY

Sequences:

>Translated_619_residues
MLRLITSMTAPTYSLTASPLPEDRRSLLMDLVKGLDTPSLWWLSGYVAGLAQSQLTSTLTAVQEEDSGTQAKQRLTVLYG
SQTGNAQRIAEQLAAEAEAAGLARRLIRADDYTPRELAAERLLYIVISTQGEGDPPDDSIGFVEFLNERRAPKLPELKYA
VLGLGDSSYADFCGIARRLDARLQALGAQRLQPLGEADVDIDTVATPWLKQALHHAREQLKNATAPLTNVTPLRGNSALR
GSNQTFQAELFKNQLVSGRDFKGPAFRTHGQASKRVHHLELSLEGSGLHYEPGDALGVHHRNPNTLVDAVLTTLKLSAHT
EVTQDKETLPLSEWLATRREVTRLSRPFLAAHAERSGAEELRALLTPTQTAGLATLLNDHQLIDVLRRWPADWDHTALVA
ALRPLTPRLYSIASSRKRVGEEVHITIDELHYQAHGHEHIGAASSFLAGLTEADTLPVYIQPNERFRLPADATRDIIMIG
SGTGVAPFRAFVQERAESGATGRNWLFFGAQHFNCDFLYQTEWQQALQRGELHALDLAFSRDHAEKIYVQHRLLAQGQEV
YTWLQNGAYLYVCGAISMSKDVHTTLLQILVTHGNLTKEAAAETLIGMQREGRYARDVY
>Mature_619_residues
MLRLITSMTAPTYSLTASPLPEDRRSLLMDLVKGLDTPSLWWLSGYVAGLAQSQLTSTLTAVQEEDSGTQAKQRLTVLYG
SQTGNAQRIAEQLAAEAEAAGLARRLIRADDYTPRELAAERLLYIVISTQGEGDPPDDSIGFVEFLNERRAPKLPELKYA
VLGLGDSSYADFCGIARRLDARLQALGAQRLQPLGEADVDIDTVATPWLKQALHHAREQLKNATAPLTNVTPLRGNSALR
GSNQTFQAELFKNQLVSGRDFKGPAFRTHGQASKRVHHLELSLEGSGLHYEPGDALGVHHRNPNTLVDAVLTTLKLSAHT
EVTQDKETLPLSEWLATRREVTRLSRPFLAAHAERSGAEELRALLTPTQTAGLATLLNDHQLIDVLRRWPADWDHTALVA
ALRPLTPRLYSIASSRKRVGEEVHITIDELHYQAHGHEHIGAASSFLAGLTEADTLPVYIQPNERFRLPADATRDIIMIG
SGTGVAPFRAFVQERAESGATGRNWLFFGAQHFNCDFLYQTEWQQALQRGELHALDLAFSRDHAEKIYVQHRLLAQGQEV
YTWLQNGAYLYVCGAISMSKDVHTTLLQILVTHGNLTKEAAAETLIGMQREGRYARDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=612, Percent_Identity=29.9019607843137, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI127139033, Length=628, Percent_Identity=28.6624203821656, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI7657393, Length=617, Percent_Identity=30.6320907617504, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI221316709, Length=617, Percent_Identity=30.6320907617504, Blast_Score=192, Evalue=1e-48,
Organism=Homo sapiens, GI221316705, Length=626, Percent_Identity=30.1916932907348, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI221316707, Length=545, Percent_Identity=30.8256880733945, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI40254422, Length=658, Percent_Identity=27.8115501519757, Blast_Score=171, Evalue=3e-42,
Organism=Homo sapiens, GI10835173, Length=653, Percent_Identity=24.6554364471669, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI169790958, Length=415, Percent_Identity=28.6746987951807, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI169790956, Length=415, Percent_Identity=28.6746987951807, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI50726981, Length=167, Percent_Identity=30.5389221556886, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789123, Length=620, Percent_Identity=38.8709677419355, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1790181, Length=116, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789154, Length=145, Percent_Identity=28.2758620689655, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554134, Length=669, Percent_Identity=26.457399103139, Blast_Score=188, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17566446, Length=580, Percent_Identity=27.0689655172414, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17531441, Length=140, Percent_Identity=40, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=42.4, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321832, Length=641, Percent_Identity=27.7691107644306, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321143, Length=378, Percent_Identity=27.5132275132275, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325305, Length=640, Percent_Identity=23.75, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322597, Length=182, Percent_Identity=35.1648351648352, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137192, Length=617, Percent_Identity=29.6596434359805, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24583543, Length=668, Percent_Identity=27.2455089820359, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI78706876, Length=668, Percent_Identity=27.2455089820359, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI78706872, Length=668, Percent_Identity=27.2455089820359, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24582192, Length=524, Percent_Identity=28.2442748091603, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24660907, Length=579, Percent_Identity=29.706390328152, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24660903, Length=579, Percent_Identity=29.706390328152, Blast_Score=162, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 68540; Mature: 68540

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLITSMTAPTYSLTASPLPEDRRSLLMDLVKGLDTPSLWWLSGYVAGLAQSQLTSTLT
CCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVQEEDSGTQAKQRLTVLYGSQTGNAQRIAEQLAAEAEAAGLARRLIRADDYTPRELAAE
HHHHCCCCHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
RLLYIVISTQGEGDPPDDSIGFVEFLNERRAPKLPELKYAVLGLGDSSYADFCGIARRLD
EEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ARLQALGAQRLQPLGEADVDIDTVATPWLKQALHHAREQLKNATAPLTNVTPLRGNSALR
HHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GSNQTFQAELFKNQLVSGRDFKGPAFRTHGQASKRVHHLELSLEGSGLHYEPGDALGVHH
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCEEEEEEEEECCCCEECCCCCCCCCC
RNPNTLVDAVLTTLKLSAHTEVTQDKETLPLSEWLATRREVTRLSRPFLAAHAERSGAEE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCHHH
LRALLTPTQTAGLATLLNDHQLIDVLRRWPADWDHTALVAALRPLTPRLYSIASSRKRVG
HHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
EEVHITIDELHYQAHGHEHIGAASSFLAGLTEADTLPVYIQPNERFRLPADATRDIIMIG
CEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCCCCEEEEE
SGTGVAPFRAFVQERAESGATGRNWLFFGAQHFNCDFLYQTEWQQALQRGELHALDLAFS
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHCCCEEEEEEHHH
RDHAEKIYVQHRLLAQGQEVYTWLQNGAYLYVCGAISMSKDVHTTLLQILVTHGNLTKEA
HHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHH
AAETLIGMQREGRYARDVY
HHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MLRLITSMTAPTYSLTASPLPEDRRSLLMDLVKGLDTPSLWWLSGYVAGLAQSQLTSTLT
CCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVQEEDSGTQAKQRLTVLYGSQTGNAQRIAEQLAAEAEAAGLARRLIRADDYTPRELAAE
HHHHCCCCHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
RLLYIVISTQGEGDPPDDSIGFVEFLNERRAPKLPELKYAVLGLGDSSYADFCGIARRLD
EEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ARLQALGAQRLQPLGEADVDIDTVATPWLKQALHHAREQLKNATAPLTNVTPLRGNSALR
HHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GSNQTFQAELFKNQLVSGRDFKGPAFRTHGQASKRVHHLELSLEGSGLHYEPGDALGVHH
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCEEEEEEEEECCCCEECCCCCCCCCC
RNPNTLVDAVLTTLKLSAHTEVTQDKETLPLSEWLATRREVTRLSRPFLAAHAERSGAEE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCHHH
LRALLTPTQTAGLATLLNDHQLIDVLRRWPADWDHTALVAALRPLTPRLYSIASSRKRVG
HHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
EEVHITIDELHYQAHGHEHIGAASSFLAGLTEADTLPVYIQPNERFRLPADATRDIIMIG
CEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCCCCEEEEE
SGTGVAPFRAFVQERAESGATGRNWLFFGAQHFNCDFLYQTEWQQALQRGELHALDLAFS
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHHHCCCEEEEEEHHH
RDHAEKIYVQHRLLAQGQEVYTWLQNGAYLYVCGAISMSKDVHTTLLQILVTHGNLTKEA
HHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHH
AAETLIGMQREGRYARDVY
HHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA