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The map label for this gene is rnhB

Identifier: 28198242

GI number: 28198242

Start: 413390

End: 414094

Strand: Reverse

Name: rnhB

Synonym: PD0321

Alternate gene names: 28198242

Gene position: 414094-413390 (Counterclockwise)

Preceding gene: 28198243

Following gene: 28198240

Centisome position: 16.43

GC content: 53.33

Gene sequence:

>705_bases
ATGGTCTCCAAGTTTGATACACAACATCTCCTCAATAGCAACACGGCACTTGTCGCTGGCGTCGACGAAGCCGGACGTGG
CCCCCTCGCCGGACCAGTCGTCGTTGCAGCAGTCGTATTCGATCCCTCACAACCACACATCAACGGCCTTAACGATTCCA
AACAATTGTCCCCTGCCTGCCGTGAACGTCTCTACGCTCACATCGTTGAGCGCGCCTTGGCCTATAAAGTAGTCATGATC
GACAGCACACAGATCGATACACTGAATATTTACCAAGCCACCATGCTCGGTATGCGCTTAGCGGTAGAAGGCGTCGCTCA
CGTTGCGAAATCCGCGAGAATTGATGGCAACCGTCTCCCTAAAAACCTCCCCTGCCCGGCTGAAGCTCTAGTCGGCGGTG
ACGCACGTGATCCCACAATCATGGCCGCTTCCATACTGGCCAAAGTCACCCGCGATCGCCATATGGTTGAACTGCATCTG
CAATATCCACATTACGGCTTCGACAAACACAAAGGGTACGGAACACCGGCACACCTGGCCGCTTTAGCAGAGCATGGCCC
ATGTCTAGAGCACCGCCAAAGCTTCGCTCCTGTGCGCAGAATGCTGACCCCTAAAGCCATCCATGCAAGACAATCAGCAC
ATCAACACAATGAGAACTCACCTACTAAAGCAGCCTTTAACATGCTCATAGAACGCGATGATTGA

Upstream 100 bases:

>100_bases
CACGCGCAGCCGAAGCAATCGCCGAACTATTACAACAGAGATAGACTGCAACACTCGGGGACACTGCACCACGAATCAGC
ACCACACACAGCAGGTAACA

Downstream 100 bases:

>100_bases
GAGCAACATCGAATAACAAGTAACTCTTCCGAAGTCACTGAAGATCACAGTGAATGCTTTCCGAGCACATAACAGACTTG
CATTCCCCCACACATTCAAC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MVSKFDTQHLLNSNTALVAGVDEAGRGPLAGPVVVAAVVFDPSQPHINGLNDSKQLSPACRERLYAHIVERALAYKVVMI
DSTQIDTLNIYQATMLGMRLAVEGVAHVAKSARIDGNRLPKNLPCPAEALVGGDARDPTIMAASILAKVTRDRHMVELHL
QYPHYGFDKHKGYGTPAHLAALAEHGPCLEHRQSFAPVRRMLTPKAIHARQSAHQHNENSPTKAAFNMLIERDD

Sequences:

>Translated_234_residues
MVSKFDTQHLLNSNTALVAGVDEAGRGPLAGPVVVAAVVFDPSQPHINGLNDSKQLSPACRERLYAHIVERALAYKVVMI
DSTQIDTLNIYQATMLGMRLAVEGVAHVAKSARIDGNRLPKNLPCPAEALVGGDARDPTIMAASILAKVTRDRHMVELHL
QYPHYGFDKHKGYGTPAHLAALAEHGPCLEHRQSFAPVRRMLTPKAIHARQSAHQHNENSPTKAAFNMLIERDD
>Mature_234_residues
MVSKFDTQHLLNSNTALVAGVDEAGRGPLAGPVVVAAVVFDPSQPHINGLNDSKQLSPACRERLYAHIVERALAYKVVMI
DSTQIDTLNIYQATMLGMRLAVEGVAHVAKSARIDGNRLPKNLPCPAEALVGGDARDPTIMAASILAKVTRDRHMVELHL
QYPHYGFDKHKGYGTPAHLAALAEHGPCLEHRQSFAPVRRMLTPKAIHARQSAHQHNENSPTKAAFNMLIERDD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=189, Percent_Identity=56.0846560846561, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_XYLF2 (B2I7N7)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829039.1
- ProteinModelPortal:   B2I7N7
- SMR:   B2I7N7
- GeneID:   6204146
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0317
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25552; Mature: 25552

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKFDTQHLLNSNTALVAGVDEAGRGPLAGPVVVAAVVFDPSQPHINGLNDSKQLSPAC
CCCCCCHHHHHCCCCEEEEECCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHCCHHH
RERLYAHIVERALAYKVVMIDSTQIDTLNIYQATMLGMRLAVEGVAHVAKSARIDGNRLP
HHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KNLPCPAEALVGGDARDPTIMAASILAKVTRDRHMVELHLQYPHYGFDKHKGYGTPAHLA
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
ALAEHGPCLEHRQSFAPVRRMLTPKAIHARQSAHQHNENSPTKAAFNMLIERDD
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSKFDTQHLLNSNTALVAGVDEAGRGPLAGPVVVAAVVFDPSQPHINGLNDSKQLSPAC
CCCCCCHHHHHCCCCEEEEECCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCHHCCHHH
RERLYAHIVERALAYKVVMIDSTQIDTLNIYQATMLGMRLAVEGVAHVAKSARIDGNRLP
HHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KNLPCPAEALVGGDARDPTIMAASILAKVTRDRHMVELHLQYPHYGFDKHKGYGTPAHLA
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
ALAEHGPCLEHRQSFAPVRRMLTPKAIHARQSAHQHNENSPTKAAFNMLIERDD
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA