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The map label for this gene is tpiA

Identifier: 62184937

GI number: 62184937

Start: 355057

End: 355821

Strand: Direct

Name: tpiA

Synonym: CAB305

Alternate gene names: 62184937

Gene position: 355057-355821 (Clockwise)

Preceding gene: 62184932

Following gene: 62184938

Centisome position: 31.03

GC content: 43.92

Gene sequence:

>765_bases
ATGGAACGTAAGCGTTATATTTTCGGTAATTGGAAAATGCATAAGACAGCTAAAGAGGCTAAAGATTATTTGTCTGTTTT
CTGCCCTCTCCTTGAAGAAGTTGCTCCTGTATCTTGTGTGGGTATCACCCCCGCATTCACAGCGTTACATGCTTGCTGTG
AATCTATAAAATTTTTTCACAGTCCTCTGTGGCTGGGAGCGCAAAATGTACATCAGGATACCTCGGGAGCCTTTACAGGT
GAAATTTCCTTGCCTATGCTCAAAGAATTTGATGTCAATTTTGTACTTTTAGGTCACTCCGAATGCCGTCATATTTTTCA
TGAAGAAGATACGACCATTGCGCTTAAGGTTGGTGCCGCAGCTCGTGAAGGCATTATTCCCGTTCTGTGTATCGGAGAAA
CTCTAGAAGTTAGAGAAAAAGGCGCGACGGAAGCTATGTTATCTAACCAGTTGATGTTGGGGCTTGCGCAACTTCCGGAA
ACTGCCTCTGTAATCATTGCTTATGAACCCGTATGGGCAATTGGTACGGGTAAAGTAGCTTCAGCGGTTGATGTGCAAGA
GGCACATGCCTTTTGTCGAGAGGTGCTTGCGAATATATTTTCCAAAGAGAAAGCTGAGGAGATTTCCATTCTTTATGGGG
GATCTGTAAAGGCAGATAATGCTGAAGGATTTGCTCGTTGTCCTGATGTAGACGGGTTGTTAGTAGGGGGCGCTTCCTTA
GATCCTAAAGTTTTTGCCGATGTTGTCGCGAATTTTCATCGTTAG

Upstream 100 bases:

>100_bases
ATTATTTTAAAATAAAGCACCTTCCTAGTAAAAAACTAGCAAGAATCGTGAAATCATGTCTATAGTGGGAGATGAACGAG
CTAACAAATAGAGGACGTAG

Downstream 100 bases:

>100_bases
CTAGTAAAAATTATATTAGCTTTTTGGCCATGTTGTGTGAAATATTTTCATAGAATTTACACCAAGGTTTTGTTTGAGAA
CAGTGTTCCAAATCAATAAA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MERKRYIFGNWKMHKTAKEAKDYLSVFCPLLEEVAPVSCVGITPAFTALHACCESIKFFHSPLWLGAQNVHQDTSGAFTG
EISLPMLKEFDVNFVLLGHSECRHIFHEEDTTIALKVGAAAREGIIPVLCIGETLEVREKGATEAMLSNQLMLGLAQLPE
TASVIIAYEPVWAIGTGKVASAVDVQEAHAFCREVLANIFSKEKAEEISILYGGSVKADNAEGFARCPDVDGLLVGGASL
DPKVFADVVANFHR

Sequences:

>Translated_254_residues
MERKRYIFGNWKMHKTAKEAKDYLSVFCPLLEEVAPVSCVGITPAFTALHACCESIKFFHSPLWLGAQNVHQDTSGAFTG
EISLPMLKEFDVNFVLLGHSECRHIFHEEDTTIALKVGAAAREGIIPVLCIGETLEVREKGATEAMLSNQLMLGLAQLPE
TASVIIAYEPVWAIGTGKVASAVDVQEAHAFCREVLANIFSKEKAEEISILYGGSVKADNAEGFARCPDVDGLLVGGASL
DPKVFADVVANFHR
>Mature_254_residues
MERKRYIFGNWKMHKTAKEAKDYLSVFCPLLEEVAPVSCVGITPAFTALHACCESIKFFHSPLWLGAQNVHQDTSGAFTG
EISLPMLKEFDVNFVLLGHSECRHIFHEEDTTIALKVGAAAREGIIPVLCIGETLEVREKGATEAMLSNQLMLGLAQLPE
TASVIIAYEPVWAIGTGKVASAVDVQEAHAFCREVLANIFSKEKAEEISILYGGSVKADNAEGFARCPDVDGLLVGGASL
DPKVFADVVANFHR

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=254, Percent_Identity=38.5826771653543, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI226529917, Length=254, Percent_Identity=38.5826771653543, Blast_Score=157, Evalue=6e-39,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=39.7590361445783, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=43.8524590163934, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=40.2390438247012, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28572008, Length=255, Percent_Identity=41.5686274509804, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI28572006, Length=255, Percent_Identity=41.5686274509804, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI28572004, Length=254, Percent_Identity=43.3070866141732, Blast_Score=181, Evalue=5e-46,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CHLAB (Q5L6H0)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219722.1
- HSSP:   P36204
- ProteinModelPortal:   Q5L6H0
- SMR:   Q5L6H0
- GeneID:   3338220
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB305
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q5L6H0
- ProtClustDB:   PRK00042
- BioCyc:   CABO218497:CAB305-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27609; Mature: 27609

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 99-99 ACT_SITE 169-169 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKRYIFGNWKMHKTAKEAKDYLSVFCPLLEEVAPVSCVGITPAFTALHACCESIKFFH
CCCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
SPLWLGAQNVHQDTSGAFTGEISLPMLKEFDVNFVLLGHSECRHIFHEEDTTIALKVGAA
CCHHCCCHHHCCCCCCCEEEEECCCHHHHCCCCEEEECCHHHHHHHCCCCCEEEEEECCH
AREGIIPVLCIGETLEVREKGATEAMLSNQLMLGLAQLPETASVIIAYEPVWAIGTGKVA
HHCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCHH
SAVDVQEAHAFCREVLANIFSKEKAEEISILYGGSVKADNAEGFARCPDVDGLLVGGASL
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCC
DPKVFADVVANFHR
CHHHHHHHHHHCCC
>Mature Secondary Structure
MERKRYIFGNWKMHKTAKEAKDYLSVFCPLLEEVAPVSCVGITPAFTALHACCESIKFFH
CCCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHH
SPLWLGAQNVHQDTSGAFTGEISLPMLKEFDVNFVLLGHSECRHIFHEEDTTIALKVGAA
CCHHCCCHHHCCCCCCCEEEEECCCHHHHCCCCEEEECCHHHHHHHCCCCCEEEEEECCH
AREGIIPVLCIGETLEVREKGATEAMLSNQLMLGLAQLPETASVIIAYEPVWAIGTGKVA
HHCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCHH
SAVDVQEAHAFCREVLANIFSKEKAEEISILYGGSVKADNAEGFARCPDVDGLLVGGASL
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCC
DPKVFADVVANFHR
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA