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The map label for this gene is ksgA

Identifier: 62184932

GI number: 62184932

Start: 349533

End: 350369

Strand: Direct

Name: ksgA

Synonym: CAB300

Alternate gene names: 62184932

Gene position: 349533-350369 (Clockwise)

Preceding gene: 62184931

Following gene: 62184937

Centisome position: 30.54

GC content: 42.29

Gene sequence:

>837_bases
TTGTCACGCAGTTCTCCTGAACAACTTAGCCGCTTTTTAGCTGAAGTTCATGGCCGTCCGAAAAAAGGCCTGTCACAGAA
CTTTTTGATAGATGGGAATATTCTAAGGAAGATCCTTGCTGTTTCTTGTGTACAGGCTGGGGATTGGGTCTTGGAAATCG
GCCCCGGATTTGGAGCTCTTACCGAAGTCCTCGTGAATCAAGGAGCTCATGTCGTTGCTTTAGAAAAGGATTCTATGTTG
GAGGAAACATTAAAGCAACTTCCCATACATCTAGAAATCACAGATGCTTGTAAATATCCTTTATCACAACTTCAAGATCA
GGGCTGGCAAGGAAAAGGACGGGTTGTTGCTAACCTGCCCTATCATATTACCACACCTTTATTAAGAAAATTATTTTTAG
AAGCTCCTAACCAGTGGAAAACGGTTACGGTCATGATCCAAGATGAAGTCGCGCGACGTATCACAGCGCAACCCGGAGGT
AAAGAATACGGATCATTAACTATCTTTTTACAATTTTTTGTTGATGTGCACTACGCTTTTAAAGTGAGCCCAGGATGCTT
TTTACCTAAACCGCAGGTAGCCTCCGCGGTTGTTCATATGACAGTAAAAGAGAACTTTCCTCTTGAAGAACCTCTCCGTA
CGAAATTCTTTTCTTTAACTCGAGCTGCATTTGGGCAGAGACGAAAACTCTTAGCGAATGCTCTTAAAGATCTTTATCCT
AAAGAACGTGTTTTTGAGGCTTTAAGCCAACTACATTTTTCTGACAAAACTCGTCCAGAGACCCTGTCTCTCGATGACTA
TCTCAAACTCTTTTATCTTCTCTCCTTGCCTGCCTAA

Upstream 100 bases:

>100_bases
AAAAGAAATTGCGTGCGCGCTACGGCTTTGACTCATCGATGATTGCTAAGTTGCTTTCTGAAGAAGCTCCACCTCTATTT
TCATTACTTTAGGAGATCAT

Downstream 100 bases:

>100_bases
GTGCACCTCAGGTTAGTGTTAAAGCAGACTTTTTCTATACAAGAGAAAATATAAATTAAATGCAGAAGATTTTAATTTAT
CTTTTTTATAGGAGATTTTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSRSSPEQLSRFLAEVHGRPKKGLSQNFLIDGNILRKILAVSCVQAGDWVLEIGPGFGALTEVLVNQGAHVVALEKDSML
EETLKQLPIHLEITDACKYPLSQLQDQGWQGKGRVVANLPYHITTPLLRKLFLEAPNQWKTVTVMIQDEVARRITAQPGG
KEYGSLTIFLQFFVDVHYAFKVSPGCFLPKPQVASAVVHMTVKENFPLEEPLRTKFFSLTRAAFGQRRKLLANALKDLYP
KERVFEALSQLHFSDKTRPETLSLDDYLKLFYLLSLPA

Sequences:

>Translated_278_residues
MSRSSPEQLSRFLAEVHGRPKKGLSQNFLIDGNILRKILAVSCVQAGDWVLEIGPGFGALTEVLVNQGAHVVALEKDSML
EETLKQLPIHLEITDACKYPLSQLQDQGWQGKGRVVANLPYHITTPLLRKLFLEAPNQWKTVTVMIQDEVARRITAQPGG
KEYGSLTIFLQFFVDVHYAFKVSPGCFLPKPQVASAVVHMTVKENFPLEEPLRTKFFSLTRAAFGQRRKLLANALKDLYP
KERVFEALSQLHFSDKTRPETLSLDDYLKLFYLLSLPA
>Mature_277_residues
SRSSPEQLSRFLAEVHGRPKKGLSQNFLIDGNILRKILAVSCVQAGDWVLEIGPGFGALTEVLVNQGAHVVALEKDSMLE
ETLKQLPIHLEITDACKYPLSQLQDQGWQGKGRVVANLPYHITTPLLRKLFLEAPNQWKTVTVMIQDEVARRITAQPGGK
EYGSLTIFLQFFVDVHYAFKVSPGCFLPKPQVASAVVHMTVKENFPLEEPLRTKFFSLTRAAFGQRRKLLANALKDLYPK
ERVFEALSQLHFSDKTRPETLSLDDYLKLFYLLSLPA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=276, Percent_Identity=27.8985507246377, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI156415992, Length=274, Percent_Identity=27.7372262773723, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=31.2977099236641, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25141369, Length=286, Percent_Identity=30.0699300699301, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25146882, Length=207, Percent_Identity=30.9178743961353, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=213, Percent_Identity=27.6995305164319, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358017, Length=308, Percent_Identity=27.9220779220779, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357273, Length=281, Percent_Identity=29.1814946619217, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CHLAB (Q5L6H5)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219717.1
- HSSP:   P06992
- ProteinModelPortal:   Q5L6H5
- SMR:   Q5L6H5
- GeneID:   3337437
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB300
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   CABO218497:CAB300-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31334; Mature: 31203

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 95-95 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSSPEQLSRFLAEVHGRPKKGLSQNFLIDGNILRKILAVSCVQAGDWVLEIGPGFGAL
CCCCCHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHH
TEVLVNQGAHVVALEKDSMLEETLKQLPIHLEITDACKYPLSQLQDQGWQGKGRVVANLP
HHHHHCCCCEEEEECCHHHHHHHHHHCCEEEEEHHHHHCCHHHHHCCCCCCCCCEEEECC
YHITTPLLRKLFLEAPNQWKTVTVMIQDEVARRITAQPGGKEYGSLTIFLQFFVDVHYAF
CHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHE
KVSPGCFLPKPQVASAVVHMTVKENFPLEEPLRTKFFSLTRAAFGQRRKLLANALKDLYP
EECCCCCCCCHHHHHHHHEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KERVFEALSQLHFSDKTRPETLSLDDYLKLFYLLSLPA
HHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRSSPEQLSRFLAEVHGRPKKGLSQNFLIDGNILRKILAVSCVQAGDWVLEIGPGFGAL
CCCCHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEECCCHHHH
TEVLVNQGAHVVALEKDSMLEETLKQLPIHLEITDACKYPLSQLQDQGWQGKGRVVANLP
HHHHHCCCCEEEEECCHHHHHHHHHHCCEEEEEHHHHHCCHHHHHCCCCCCCCCEEEECC
YHITTPLLRKLFLEAPNQWKTVTVMIQDEVARRITAQPGGKEYGSLTIFLQFFVDVHYAF
CHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHE
KVSPGCFLPKPQVASAVVHMTVKENFPLEEPLRTKFFSLTRAAFGQRRKLLANALKDLYP
EECCCCCCCCHHHHHHHHEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KERVFEALSQLHFSDKTRPETLSLDDYLKLFYLLSLPA
HHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA