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The map label for this gene is gltX

Identifier: 62184823

GI number: 62184823

Start: 196465

End: 197982

Strand: Direct

Name: gltX

Synonym: CAB179

Alternate gene names: 62184823

Gene position: 196465-197982 (Clockwise)

Preceding gene: 62184822

Following gene: 62184824

Centisome position: 17.17

GC content: 44.01

Gene sequence:

>1518_bases
ATGGCTTGGGAAAACGTACGTGTTAGAGTTGCGCCGTCGCCTACAGGGGATCCCCACGTAGGGACAGCCTATATGGCTTT
GTTTAATGCAATCTTTGCAAAACGATTCAATGGGAAGATGATCCTAAGAATCGAAGATACGGATCAAACACGTAGCCGTG
ATGATTATGAAAAAAATATTTTCTCCGCACTGCAGTGGTGCGGGATTCAGTGGGATGAAGGACCCGATATAGGTGGCCCC
TACGGCCCCTATAGACAGTCGGAACGTACTGAAATCTATAGAAAATATGCTGAACTCCTTTTAAAAACAGACTACGCCTA
CAAGTGTTTTGCTACACCTAAAGAACTTGAGGAAATGCGAGCTGTAGCCACTACTCTAGGATATCGCGGAGGCTATGATC
GCAGGTACCGGTATCTTTCTTCTGAGGAGGTAGACGCTCGTACTCGAGAAGGACAACCATACACTATTCGTTTGAAAGTT
CCCCTTACCGGGGAATGCGTTCTTGACGATTACTGCAAGGGTAGGGTGGTTTTCCCCTGGGCAGACGTAGATGATCAGGT
TTTGATCAAATCCGATGGCTTCCCTACCTACCACTTTGCTAATGTCGTCGATGATCATCTCATGGGCATCACTCATGTCC
TGCGTGGAGAGGAGTGGCTGAGTTCGACTCCTAAGCACCTGCTTCTTTACCAAGCTTTTGGTTGGAAAGCTCCTACTTTC
CTCCACATGCCACTACTTCTTAATCCTGACGGGACAAAACTTTCTAAAAGAAAAAATCCTACGTCCATCTTCTATTACCG
AGACGCTGGTTACATCAAAGAAGCCTTCATGAACTTCCTTACCCTGATGGGGTACAGCATGGAGGGGGATGAGGAGATCT
ATTCTCTAGAGAAGCTTATCGCAAACTTCGACCCTCGACGTATTGGAAAGTCGGGAGCAGTTTTCGATACCCGAAAACTA
GATTGGATGAATAAGCATTACCTCACTCATGAAAGGTCGTCGGAAAGCCTATTAGCTAAACTAAAAGATTGGCTAATCAA
TGATGAGTTTTTCTTGAAAATTCTCCCACTCTGTCAGTCTCGCATTACCACTCTTGCCGAATTCATCGGATTTACCGGAT
TTTTCTTTTCCGTTCTTCCCGAATATTCAAAAGAGGAGCTGTTACCTACAACGATTTCTCAGGAGAAGGCGGCTATTCTT
CTTTATAGTTATGTCAAATACTTAGAAAAATCCGATCTATGGGTAAAAGACCAGTTTTATCAAGGGTCAAAATGGCTGTC
ATCAGCTTTCCAAGTACACCATAAGAAGGTCGTTATCCCTTTACTTTATGTAGCGATTACAGGAAAGAAGCAGGGATTAC
CTTTATTTGACTCTATGGAGTTATTAGGAAAACCTCGTACACGCGCCCGTTTGGTTCATGCGCAAAATCTCCTCGGAGGC
GTACCTAAGAAAATCCAAACGACCATCGATAAAGTTCTGAAAGAAGAAGATCTAGAAAGTAAGATTTTCGAGTTTTAA

Upstream 100 bases:

>100_bases
TCGTCTCTAATTTTGTTGAGACAACCTAGAGAAGGATTTCTCTTGAAGAAATAACTAGGTTCAAGGTATCAAGTCTCTTT
CGTCTTAGGATTAAATGAGA

Downstream 100 bases:

>100_bases
GACTTTTTACCTAGCCATCACTCACAAATGAGATTTTACCCAACAGAACTAGATGTTTTGTGGGTAAGAGGCATGGAAAA
TGGACTTGGCTACAGAAAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MAWENVRVRVAPSPTGDPHVGTAYMALFNAIFAKRFNGKMILRIEDTDQTRSRDDYEKNIFSALQWCGIQWDEGPDIGGP
YGPYRQSERTEIYRKYAELLLKTDYAYKCFATPKELEEMRAVATTLGYRGGYDRRYRYLSSEEVDARTREGQPYTIRLKV
PLTGECVLDDYCKGRVVFPWADVDDQVLIKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYQAFGWKAPTF
LHMPLLLNPDGTKLSKRKNPTSIFYYRDAGYIKEAFMNFLTLMGYSMEGDEEIYSLEKLIANFDPRRIGKSGAVFDTRKL
DWMNKHYLTHERSSESLLAKLKDWLINDEFFLKILPLCQSRITTLAEFIGFTGFFFSVLPEYSKEELLPTTISQEKAAIL
LYSYVKYLEKSDLWVKDQFYQGSKWLSSAFQVHHKKVVIPLLYVAITGKKQGLPLFDSMELLGKPRTRARLVHAQNLLGG
VPKKIQTTIDKVLKEEDLESKIFEF

Sequences:

>Translated_505_residues
MAWENVRVRVAPSPTGDPHVGTAYMALFNAIFAKRFNGKMILRIEDTDQTRSRDDYEKNIFSALQWCGIQWDEGPDIGGP
YGPYRQSERTEIYRKYAELLLKTDYAYKCFATPKELEEMRAVATTLGYRGGYDRRYRYLSSEEVDARTREGQPYTIRLKV
PLTGECVLDDYCKGRVVFPWADVDDQVLIKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYQAFGWKAPTF
LHMPLLLNPDGTKLSKRKNPTSIFYYRDAGYIKEAFMNFLTLMGYSMEGDEEIYSLEKLIANFDPRRIGKSGAVFDTRKL
DWMNKHYLTHERSSESLLAKLKDWLINDEFFLKILPLCQSRITTLAEFIGFTGFFFSVLPEYSKEELLPTTISQEKAAIL
LYSYVKYLEKSDLWVKDQFYQGSKWLSSAFQVHHKKVVIPLLYVAITGKKQGLPLFDSMELLGKPRTRARLVHAQNLLGG
VPKKIQTTIDKVLKEEDLESKIFEF
>Mature_504_residues
AWENVRVRVAPSPTGDPHVGTAYMALFNAIFAKRFNGKMILRIEDTDQTRSRDDYEKNIFSALQWCGIQWDEGPDIGGPY
GPYRQSERTEIYRKYAELLLKTDYAYKCFATPKELEEMRAVATTLGYRGGYDRRYRYLSSEEVDARTREGQPYTIRLKVP
LTGECVLDDYCKGRVVFPWADVDDQVLIKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYQAFGWKAPTFL
HMPLLLNPDGTKLSKRKNPTSIFYYRDAGYIKEAFMNFLTLMGYSMEGDEEIYSLEKLIANFDPRRIGKSGAVFDTRKLD
WMNKHYLTHERSSESLLAKLKDWLINDEFFLKILPLCQSRITTLAEFIGFTGFFFSVLPEYSKEELLPTTISQEKAAILL
YSYVKYLEKSDLWVKDQFYQGSKWLSSAFQVHHKKVVIPLLYVAITGKKQGLPLFDSMELLGKPRTRARLVHAQNLLGGV
PKKIQTTIDKVLKEEDLESKIFEF

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=37.5510204081633, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI62241042, Length=255, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=31.2757201646091, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI226510925, Length=296, Percent_Identity=30.7432432432432, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=33.4702258726899, Blast_Score=261, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6324540, Length=504, Percent_Identity=35.7142857142857, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6321192, Length=240, Percent_Identity=26.25, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=38.5858585858586, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24649466, Length=272, Percent_Identity=25.3676470588235, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHLAB (Q5L6T4)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219608.1
- ProteinModelPortal:   Q5L6T4
- SMR:   Q5L6T4
- GeneID:   3337734
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB179
- HOGENOM:   HBG628189
- OMA:   MEGVPIM
- PhylomeDB:   Q5L6T4
- ProtClustDB:   PRK01406
- BioCyc:   CABO218497:CAB179-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58456; Mature: 58325

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWENVRVRVAPSPTGDPHVGTAYMALFNAIFAKRFNGKMILRIEDTDQTRSRDDYEKNI
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
FSALQWCGIQWDEGPDIGGPYGPYRQSERTEIYRKYAELLLKTDYAYKCFATPKELEEMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
AVATTLGYRGGYDRRYRYLSSEEVDARTREGQPYTIRLKVPLTGECVLDDYCKGRVVFPW
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCEEEEEE
ADVDDQVLIKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYQAFGWKAPTF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCE
LHMPLLLNPDGTKLSKRKNPTSIFYYRDAGYIKEAFMNFLTLMGYSMEGDEEIYSLEKLI
EEECEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ANFDPRRIGKSGAVFDTRKLDWMNKHYLTHERSSESLLAKLKDWLINDEFFLKILPLCQS
HCCCHHHCCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RITTLAEFIGFTGFFFSVLPEYSKEELLPTTISQEKAAILLYSYVKYLEKSDLWVKDQFY
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHHHH
QGSKWLSSAFQVHHKKVVIPLLYVAITGKKQGLPLFDSMELLGKPRTRARLVHAQNLLGG
CCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
VPKKIQTTIDKVLKEEDLESKIFEF
CCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AWENVRVRVAPSPTGDPHVGTAYMALFNAIFAKRFNGKMILRIEDTDQTRSRDDYEKNI
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
FSALQWCGIQWDEGPDIGGPYGPYRQSERTEIYRKYAELLLKTDYAYKCFATPKELEEMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
AVATTLGYRGGYDRRYRYLSSEEVDARTREGQPYTIRLKVPLTGECVLDDYCKGRVVFPW
HHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCEEEEEE
ADVDDQVLIKSDGFPTYHFANVVDDHLMGITHVLRGEEWLSSTPKHLLLYQAFGWKAPTF
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCCCE
LHMPLLLNPDGTKLSKRKNPTSIFYYRDAGYIKEAFMNFLTLMGYSMEGDEEIYSLEKLI
EEECEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ANFDPRRIGKSGAVFDTRKLDWMNKHYLTHERSSESLLAKLKDWLINDEFFLKILPLCQS
HCCCHHHCCCCCCEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RITTLAEFIGFTGFFFSVLPEYSKEELLPTTISQEKAAILLYSYVKYLEKSDLWVKDQFY
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHHHH
QGSKWLSSAFQVHHKKVVIPLLYVAITGKKQGLPLFDSMELLGKPRTRARLVHAQNLLGG
CCHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
VPKKIQTTIDKVLKEEDLESKIFEF
CCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA