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The map label for this gene is rho [H]

Identifier: 62184774

GI number: 62184774

Start: 137441

End: 138835

Strand: Direct

Name: rho [H]

Synonym: CAB129

Alternate gene names: 62184774

Gene position: 137441-138835 (Clockwise)

Preceding gene: 62184773

Following gene: 161501896

Centisome position: 12.01

GC content: 40.65

Gene sequence:

>1395_bases
ATGAAAGAAGAACGTTCTTCAGAAGTCTTGCCAAAGGTCAAAGAGAATAGAAAACATGCGTGTCCTGGGTTGCAAGAGAA
GTCTTTTGCGGGAGAATGTGCAGTAGTTGCGGATGTAGTTAATGAAAATCAACCTGTAACTATTACAAAAATTGCCAAAT
TGCAAAGGATGGGAATTGAAGAGCTTAATGTATTGGCTCGTCAATATGGAGTGAAGAACATCGGGTCTCTCACTAAATCT
CAAGTGGTATTTGAGATCGTTAAAGCAAAGTCTGAACGTTCAGATGAGCTTTTAATCGGAGAGGGAGTTTTGGAAGTTCT
TCCTGATGGGTTTGGCTTTTTAAGATCACCTACCTATAACTATCTCCCTTCTGCTGAAGATATTTATGTTTCCCCAGCTC
AAATCCGCAGATTTGATTTAAAGAAGGGAGACACAATTATAGGCACAATACGTTCTCCAAAAGAGAAAGAAAAGTATTTT
GCTTTATTAAAGGTAGATAAGATCAATGGATCTACTCCAGACAAAGCTAAGGAACGAGTCTTATTCGAAAACTTGACCCC
TCTATACCCAAATGAAAGAATTGTTATGGAAATGGGGAAGGAGCACCTCGCTGAGCGAGTGTTAGATCTTACGGCACCCA
TAGGGAAAGGACAAAGGGGACTTATTGTAGCGCCTCCACGTTCTGGAAAGACAGTAATTTTACAGAGCATAGCGCACGCT
ATTGCAGTCAACAACCCGGATATCGTTCTCATTGTTTTGTTAATTGACGAGCGCCCAGAAGAAGTGACCGATATGATTCG
ACAGGTGCGCGGTGAAGTTGTGGCTTCTACGTTTGATGAGCAGCCTGAAAGACATATTCAAGTTGCAGAAATGGTTATAG
AAAAAGCTCGACGTTTAGTTGAACACGGAAAGGATGTCGTGATCCTTCTTGATTCTATTACACGTTTAGCGCGAGCTTAC
AATACCGTGCAACCACATTCTGGAAAGATTTTAACAGGCGGCGTAGACGCGAGTGCTCTACATAAGCCTAAGAGATTTTT
CGGAGCAGCAAGAAATATCGAAGGCGGCGGATCATTAACCATCTTAGCTACTGCGTTGATAGATACCGGTTCTAGAATGG
ATGAAGTCATTTTTGAAGAGTTCAAAGGTACAGGAAATATGGAACTGGTATTAGACCGTCGTCTTTCTGATCGAAGAACC
TACCCAGCAATTGATCTTATTAAGAGTGGGACAAGAAAAGAAGAATTACTTTATCACCCTAGTGAATTGGAAAAAGTTTA
CCTTTTCCGACAGGCAATTGCAGATCTTACTGCGATTGACGCTATGCATCTGCTATTAGGTAGATTAAAAAAGACAAATA
GTAACGCAGAATTTTTACTTTCTTTAAAAGAATAG

Upstream 100 bases:

>100_bases
AAAAAATGATGCATGCCGATATTGTTATCGAAAATAACGGTACTAAAGAAGAATTACGTCATAAAGTTGAAGAATATTTT
TACGCTTTAAAGGGAGCACT

Downstream 100 bases:

>100_bases
GCTGAAAGGTCACACTACTTCTATTTTTTAACACCATCTACATGTTAGCTGAGAATCCTGAAGCCTACAGTCTTAACCAT
AAGACTGTAGATCTTGTTCT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKEERSSEVLPKVKENRKHACPGLQEKSFAGECAVVADVVNENQPVTITKIAKLQRMGIEELNVLARQYGVKNIGSLTKS
QVVFEIVKAKSERSDELLIGEGVLEVLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYF
ALLKVDKINGSTPDKAKERVLFENLTPLYPNERIVMEMGKEHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLVEHGKDVVILLDSITRLARAY
NTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRT
YPAIDLIKSGTRKEELLYHPSELEKVYLFRQAIADLTAIDAMHLLLGRLKKTNSNAEFLLSLKE

Sequences:

>Translated_464_residues
MKEERSSEVLPKVKENRKHACPGLQEKSFAGECAVVADVVNENQPVTITKIAKLQRMGIEELNVLARQYGVKNIGSLTKS
QVVFEIVKAKSERSDELLIGEGVLEVLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYF
ALLKVDKINGSTPDKAKERVLFENLTPLYPNERIVMEMGKEHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLVEHGKDVVILLDSITRLARAY
NTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRT
YPAIDLIKSGTRKEELLYHPSELEKVYLFRQAIADLTAIDAMHLLLGRLKKTNSNAEFLLSLKE
>Mature_464_residues
MKEERSSEVLPKVKENRKHACPGLQEKSFAGECAVVADVVNENQPVTITKIAKLQRMGIEELNVLARQYGVKNIGSLTKS
QVVFEIVKAKSERSDELLIGEGVLEVLPDGFGFLRSPTYNYLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYF
ALLKVDKINGSTPDKAKERVLFENLTPLYPNERIVMEMGKEHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLVEHGKDVVILLDSITRLARAY
NTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRT
YPAIDLIKSGTRKEELLYHPSELEKVYLFRQAIADLTAIDAMHLLLGRLKKTNSNAEFLLSLKE

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=61.5384615384615, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1788251, Length=277, Percent_Identity=28.158844765343, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 51733; Mature: 51733

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEERSSEVLPKVKENRKHACPGLQEKSFAGECAVVADVVNENQPVTITKIAKLQRMGIE
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCHH
ELNVLARQYGVKNIGSLTKSQVVFEIVKAKSERSDELLIGEGVLEVLPDGFGFLRSPTYN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCHHHCCCCCC
YLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYFALLKVDKINGSTPDKAKERV
CCCCCCCEEECHHHHHHEECCCCCEEEEECCCCHHHHHEEEEEEEEECCCCCCHHHHHHH
LFENLTPLYPNERIVMEMGKEHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHH
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLV
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLT
HCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHHHHHHHCCCCCCCCEE
ILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRTYPAIDLIKSGTRKEELLYHP
EEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHEECH
SELEKVYLFRQAIADLTAIDAMHLLLGRLKKTNSNAEFLLSLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure
MKEERSSEVLPKVKENRKHACPGLQEKSFAGECAVVADVVNENQPVTITKIAKLQRMGIE
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCHH
ELNVLARQYGVKNIGSLTKSQVVFEIVKAKSERSDELLIGEGVLEVLPDGFGFLRSPTYN
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCHHHCCCCCC
YLPSAEDIYVSPAQIRRFDLKKGDTIIGTIRSPKEKEKYFALLKVDKINGSTPDKAKERV
CCCCCCCEEECHHHHHHEECCCCCEEEEECCCCHHHHHEEEEEEEEECCCCCCHHHHHHH
LFENLTPLYPNERIVMEMGKEHLAERVLDLTAPIGKGQRGLIVAPPRSGKTVILQSIAHA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHH
IAVNNPDIVLIVLLIDERPEEVTDMIRQVRGEVVASTFDEQPERHIQVAEMVIEKARRLV
HHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EHGKDVVILLDSITRLARAYNTVQPHSGKILTGGVDASALHKPKRFFGAARNIEGGGSLT
HCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCHHHHHHHCCCCCCCCEE
ILATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLSDRRTYPAIDLIKSGTRKEELLYHP
EEHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHEECH
SELEKVYLFRQAIADLTAIDAMHLLLGRLKKTNSNAEFLLSLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]