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The map label for this gene is rpoA

Identifier: 62184758

GI number: 62184758

Start: 119032

End: 120162

Strand: Direct

Name: rpoA

Synonym: CAB113

Alternate gene names: 62184758

Gene position: 119032-120162 (Clockwise)

Preceding gene: 62184757

Following gene: 62184759

Centisome position: 10.4

GC content: 37.14

Gene sequence:

>1131_bases
ATGTCGGATTGTTCACAAAATTTACTTTACGATAAATTTGAATTGCCAGAGTCAGTCAAAATGATGGCTGTAGAAGGTAG
CGGGGGATCAGTTGATAAGCAGGCGAGTTTCATAGCAGAGCCTTTAGAAAGAGGTATGGGTCATACTTTAGGTAATGCTT
TGAGAAGAGCTTTGCTCATAGGTTTAGAAGCTCCTGCAATTATTTCTTTCTCTATGACTGGTGTGCTTCATGAGTACATG
GCAATTAACGGAATTATAGAAGATGTAACAAATATTATCTTGAATTTGAAGGGAGCTTTATTAAAGAAGTATCCTTTCCA
AGACAGTGAAAATGGCCGTTGTACTCAATTATTAAAGTCTAAAGTTTCTATAGACGCTTCTGATTTAGCTGCCTGTGGTG
GTCAGAAAGCTGTTACATTGGCTGATTTGTTGCAAGAAGGCGGGTTTGAATCCGTGAACCCCGATTATGTGATTTTCACT
GTGACACAGCCTATGCAATTAGATATTACTTTAAGAGTTGCTTTTGGCAGAGGTTATACTACATCTGAAAGAATTGTCCT
TGAGGATAAGGGTGTAAATGAAATTGTTTTAGACGCAGCTTTCTCACCCGTTGTCTTGGTAAATTACTTTGTAGAAGATA
CTCGTGTCGGTCAAGATACAGATTTCGATCGTTTGATTTTGCATGTAGAAACAGATGGTAGAGTATCCCCTAAGGAAGCT
TTAGCCTTTTCTACACAAATTTTAACCAAGCATTTCTCCATTTTTGAAAAAATGGATGAAAAGAAGATTGTCTTTGAAGA
AGCCATTTCTCTCGAAAAAGAAAATAAAGACGATATTCTTCATAAGTTAGTTTTAGGTATTAACGAGATCGAGTTATCTG
TAAGATCCACAAACTGTTTGTCTAATGCAAACATTGAAACCATAGGGGAATTGGTGATTATGCCAGAGCCTCGCTTGCTA
CAGTTCAGAAACTTTGGTAAAAAGTCTCTCTGTGAGATTAAAAACAAGTTAAAAGAAATGAAGTTAGAATTGGGCATGGA
TCTTAGTCAGTTCGGCGTTGGTTTGGACAACGTAAAAGAAAAAATGAAGTGGTATGCCGATAAGATTCGGTCTAAAAACG
GTAAGGGATAA

Upstream 100 bases:

>100_bases
GTTTAGTTGTTTCTGTCATCCGTGACGAAACTCCTGTTCCTCACAATGGTTGTCGGCCAAGAAAAAGGCGCAGAGTGTAG
TTTTAGGGGAGGAGAAAGGG

Downstream 100 bases:

>100_bases
TTAAGTCTATGCAACACGCTAGAAAGAAATTTAGAGTTGGTCGTACTTCTGCGCATAATCGTTGTATGTTAGCTAACATG
TTAAAATCTCTAATCCACCA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSDCSQNLLYDKFELPESVKMMAVEGSGGSVDKQASFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFSMTGVLHEYM
AINGIIEDVTNIILNLKGALLKKYPFQDSENGRCTQLLKSKVSIDASDLAACGGQKAVTLADLLQEGGFESVNPDYVIFT
VTQPMQLDITLRVAFGRGYTTSERIVLEDKGVNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFDRLILHVETDGRVSPKEA
LAFSTQILTKHFSIFEKMDEKKIVFEEAISLEKENKDDILHKLVLGINEIELSVRSTNCLSNANIETIGELVIMPEPRLL
QFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKEKMKWYADKIRSKNGKG

Sequences:

>Translated_376_residues
MSDCSQNLLYDKFELPESVKMMAVEGSGGSVDKQASFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFSMTGVLHEYM
AINGIIEDVTNIILNLKGALLKKYPFQDSENGRCTQLLKSKVSIDASDLAACGGQKAVTLADLLQEGGFESVNPDYVIFT
VTQPMQLDITLRVAFGRGYTTSERIVLEDKGVNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFDRLILHVETDGRVSPKEA
LAFSTQILTKHFSIFEKMDEKKIVFEEAISLEKENKDDILHKLVLGINEIELSVRSTNCLSNANIETIGELVIMPEPRLL
QFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKEKMKWYADKIRSKNGKG
>Mature_375_residues
SDCSQNLLYDKFELPESVKMMAVEGSGGSVDKQASFIAEPLERGMGHTLGNALRRALLIGLEAPAIISFSMTGVLHEYMA
INGIIEDVTNIILNLKGALLKKYPFQDSENGRCTQLLKSKVSIDASDLAACGGQKAVTLADLLQEGGFESVNPDYVIFTV
TQPMQLDITLRVAFGRGYTTSERIVLEDKGVNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFDRLILHVETDGRVSPKEAL
AFSTQILTKHFSIFEKMDEKKIVFEEAISLEKENKDDILHKLVLGINEIELSVRSTNCLSNANIETIGELVIMPEPRLLQ
FRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKEKMKWYADKIRSKNGKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=37.5776397515528, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CHLAB (Q5L6Z9)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_219543.1
- ProteinModelPortal:   Q5L6Z9
- GeneID:   3338100
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB113
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   CABO218497:CAB113-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 41766; Mature: 41635

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCSQNLLYDKFELPESVKMMAVEGSGGSVDKQASFIAEPLERGMGHTLGNALRRALLI
CCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLEAPAIISFSMTGVLHEYMAINGIIEDVTNIILNLKGALLKKYPFQDSENGRCTQLLKS
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KVSIDASDLAACGGQKAVTLADLLQEGGFESVNPDYVIFTVTQPMQLDITLRVAFGRGYT
HHCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEEECCCCC
TSERIVLEDKGVNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFDRLILHVETDGRVSPKEA
CCCEEEEECCCCCEEEHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHH
LAFSTQILTKHFSIFEKMDEKKIVFEEAISLEKENKDDILHKLVLGINEIELSVRSTNCL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCC
SNANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKE
CCCCHHHHCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
KMKWYADKIRSKNGKG
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDCSQNLLYDKFELPESVKMMAVEGSGGSVDKQASFIAEPLERGMGHTLGNALRRALLI
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GLEAPAIISFSMTGVLHEYMAINGIIEDVTNIILNLKGALLKKYPFQDSENGRCTQLLKS
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KVSIDASDLAACGGQKAVTLADLLQEGGFESVNPDYVIFTVTQPMQLDITLRVAFGRGYT
HHCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEEECCCCC
TSERIVLEDKGVNEIVLDAAFSPVVLVNYFVEDTRVGQDTDFDRLILHVETDGRVSPKEA
CCCEEEEECCCCCEEEHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHH
LAFSTQILTKHFSIFEKMDEKKIVFEEAISLEKENKDDILHKLVLGINEIELSVRSTNCL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCC
SNANIETIGELVIMPEPRLLQFRNFGKKSLCEIKNKLKEMKLELGMDLSQFGVGLDNVKE
CCCCHHHHCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
KMKWYADKIRSKNGKG
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA