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The map label for this gene is cspR [H]

Identifier: 62184725

GI number: 62184725

Start: 97712

End: 98182

Strand: Direct

Name: cspR [H]

Synonym: CAB081

Alternate gene names: 62184725

Gene position: 97712-98182 (Clockwise)

Preceding gene: 62184724

Following gene: 62184727

Centisome position: 8.54

GC content: 42.68

Gene sequence:

>471_bases
ATGAAGGTAGTTCTTTATCATCCTGATATTCCCCAAAACACGGGGAATATAGGAAGGACGTGTATAGCTCTAGGTGCTGA
ATTGATTTTAGTGAGGCCCCTAGGGTTTTCTTTATTAGATAAATTTGTTAAACGCGCGGGGATGGACTACTGGGATAAGG
TAAATCTGTCCGTTGTGGACTCTTTAGATGAAGTGCTATTCGGGGTCGATGAAGATAAGATTTTCTGTCTTTCGACCAAA
GGCGCCCAATACTACGGGGACGTGGCTCTTCCTATGGATGGTGTGTACGTATTCGGCTCTGAATCCAAAGGTTTGCCAGA
AGAAGTGTTAAAAAAATACTACAACCACACGTATTACCTGCCTATGCAACCTGGAATCAGGTCTTTAAATTTGGCAACCA
CCGTGGGTGTAGTACTCTTTGAAGCAGTTCGACAAAATAATCAAAGTACGTGTATCCGTACTGGGAATTAA

Upstream 100 bases:

>100_bases
AATTGCCTCCAAACTCTTTGTTAATTTTCGAAATTAATTTAATTGAAACTACAGAAGATACTGTAGCATTACCAGACACA
GAGGACAAAAACACAGCTTC

Downstream 100 bases:

>100_bases
GCGTGCTGATTAATCAATTTAATTAATGCATCCTTATCTTTCAATCCAACCGTACGGTCTACTTCTTTGCCATCTTTGAA
CAAAATCAAAGTTGGGATTG

Product: putative rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKVVLYHPDIPQNTGNIGRTCIALGAELILVRPLGFSLLDKFVKRAGMDYWDKVNLSVVDSLDEVLFGVDEDKIFCLSTK
GAQYYGDVALPMDGVYVFGSESKGLPEEVLKKYYNHTYYLPMQPGIRSLNLATTVGVVLFEAVRQNNQSTCIRTGN

Sequences:

>Translated_156_residues
MKVVLYHPDIPQNTGNIGRTCIALGAELILVRPLGFSLLDKFVKRAGMDYWDKVNLSVVDSLDEVLFGVDEDKIFCLSTK
GAQYYGDVALPMDGVYVFGSESKGLPEEVLKKYYNHTYYLPMQPGIRSLNLATTVGVVLFEAVRQNNQSTCIRTGN
>Mature_156_residues
MKVVLYHPDIPQNTGNIGRTCIALGAELILVRPLGFSLLDKFVKRAGMDYWDKVNLSVVDSLDEVLFGVDEDKIFCLSTK
GAQYYGDVALPMDGVYVFGSESKGLPEEVLKKYYNHTYYLPMQPGIRSLNLATTVGVVLFEAVRQNNQSTCIRTGN

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=147, Percent_Identity=38.0952380952381, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17359; Mature: 17359

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLYHPDIPQNTGNIGRTCIALGAELILVRPLGFSLLDKFVKRAGMDYWDKVNLSVVD
CEEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCHHHCCHHHHH
SLDEVLFGVDEDKIFCLSTKGAQYYGDVALPMDGVYVFGSESKGLPEEVLKKYYNHTYYL
HHHHHHHCCCCCCEEEEECCCCCEECCEECCCCCEEEECCCCCCCHHHHHHHHHCCEEEE
PMQPGIRSLNLATTVGVVLFEAVRQNNQSTCIRTGN
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MKVVLYHPDIPQNTGNIGRTCIALGAELILVRPLGFSLLDKFVKRAGMDYWDKVNLSVVD
CEEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCHHHCCHHHHH
SLDEVLFGVDEDKIFCLSTKGAQYYGDVALPMDGVYVFGSESKGLPEEVLKKYYNHTYYL
HHHHHHHCCCCCCEEEEECCCCCEECCEECCCCCEEEECCCCCCCHHHHHHHHHCCEEEE
PMQPGIRSLNLATTVGVVLFEAVRQNNQSTCIRTGN
ECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]