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The map label for this gene is clpB

Identifier: 28572899

GI number: 28572899

Start: 854662

End: 856761

Strand: Reverse

Name: clpB

Synonym: TW758

Alternate gene names: 28572899

Gene position: 856761-854662 (Counterclockwise)

Preceding gene: 28572900

Following gene: 28572898

Centisome position: 92.53

GC content: 46.71

Gene sequence:

>2100_bases
ATGTCTGAATCACAGTCTGAATTAGACAAGTACGGCACGAACCTCACAAAGACTGCGGCAACAAACAAGCTCGATCCGGT
GATAGGCCGCGATGCAGAGATCCGGCGGGTGTGTCAGATTCTCTCCAGACGAACTAAGAATAACCCCGTTCTGATAGGGT
CAGCAGGTGTTGGTAAAACTGCTATAGTTGAGGGCCTTGCGCAGCGTATTGTTGCAGGGGATGTTCCTGAGAGTTTGCGG
AACAAGGAAATAGTTAGTCTCGATTTATCGGCCCTGCTTGCTGGTGCATCTTACAGGGGAGAGTTCGAGAAACGTGTCAA
AAATCTTTTGGAAGAGATACAAAAGTCTAAGGTAATAATCTTCATAGATGAGGTTCACACACTTATGAGTGCCGGCGCAG
CAGAAGGTGCAATTGCGGCGGGTAATATGCTAAAACCCCTTTTGGCTCGAGGGGAACTCAGGCTTATTGGCGCGACCACT
CTGGATGAATATCGTGAGCATTTGGAAAAAGACCCTGCACTGGAAAGACGATTCCAGCAAGTGTTTGTTGGTGAGCCAAA
TCTTGAAGACTGCATAGCGATTATGCGGGGCTTGAAAGAGCGTTACGAGGCTCATCATAAAGTGAGTATATCCGACACAG
CACTTGTGACTGCCGTTGAGTTGTCGAGTCGATATATAACGGGCAGGCAACTTCCTGATAAGGCAATCGACTTGCTCGAC
GAGGCAGCCAGTTCATTGCGCATGGAAATTGATTCATCACCGGTTGAGCTTGATCAGCTTCGTAGGGAGGTTGATCGGTT
GCGACTCGAGGAGTTGGCATTAAAGAGTGAGAATGACCCTACGAGTGAACAACGTCTAAGATCTATAACTAACCAACTTT
CTGGCAAGCAAGAAATGTTGAATACATTACAGGCAAGCTGGAACTCCGAGAGGGCGCGTCTGAATCAGATTGGCGCTTTG
AAGGAGCAGATTGATATTGCGAAACAATCTGCAGATATTGCCCAGCGCGAGGGTCGCCTTGAAGATGCATCACGACTTCT
TTATGCAACAATTCCACAATTGCAGAAAAATTTGGCGGAGAAGTTGGAAGAACAGGACTCTGCCCCGCTTGTCAGTGACC
AGGTAATGCCGGAAGATATTGCGTCTGTTGTTGAGGGATGGACAGGTATTCCTGTAAAAAAGCTTATGCAAAAAGATGCC
AAACAGCTGCTGCATCTTGAGGAGGATTTGTCCAAATCTGTTATAGCCCAGAAGGTAGCCATAGGTGTTATTGCGGATGC
AGTACGGCGTTCGCGCGCGGGACTGTCGGATCCAAACCGACCTTCCGGAACTTTTTTATTTCTCGGCCCAACGGGGGTTG
GAAAAACACAACTGGTTAAAGCTTTAGCATCTTTGTTATACGATGGTGAAATTGTAAGAATTGACATGTCGGAGTATTCA
GAAAAATTCTCTATCTCACGTCTTATTGGCGCTCCCCCGGGGTATATCGGTCATGAAAGCGCTGGACAGCTTACTGAGTC
TGTTAGGCGGCGGCCATATTCAGTTGTATTGTTTGATGAAGCAGAAAAAGCGCATCCTGAGGTTTTTGATATCCTACTGC
AGGTTCTGGATGAAGGACGCCTAACAGACTCTCATGGTCGCACTGTTGACTTTAGAAACACGATTATAGTTTTAACTTCT
AATATAGGCTCGCGTTACCTATCAGACATTTCCCTTGAGGCTACTACAGCGCACGAGTATGTTAATGATGAGGTTCGCAG
AACCTTTCGTCCAGAATTTTTGAACAGACTCGATGAAATTGTTATTTTTGAGCCATTGTCGCAAAGTGATATTTGTCAAA
TTGTTGACCTGAATATAGAATCCCTGAATAAGCGGATCAAGGATAGGCGGATTGTTGTGACAGTCAGTGAGGATTTAAGG
CGTTGGCTGTCAAAGAGCGGCTATGACGTGATTTACGGCGCGCGGCCATTGAGGAGGCTTATTCAGCGTGAGATAGAAGA
TAGACTTGCAAAGTTGATTATAGAGGGTCTTATACATGACGGACAGACTGCGTCTTTTGACCTGTCTGGGGGCGCTGTGA
ATGCCCAGGTGTGCTCCTGA

Upstream 100 bases:

>100_bases
CGTCAGGGAAGAATTTTTGCGGCGGGTCAGGAGATAGTAACTTTAGAATACGGCACAAGGATAAAGCGGGGAAAGGCCCT
TATTGTGTGGCGGGAGTTTC

Downstream 100 bases:

>100_bases
GTAATCTGCGTCGCGTATCAGATGCTTCCGGAAAGGGCCGAAATGCATTGCGTTTGTTCTGCCCTCGCGGGCGTTTTTTC
AGAAATACTGCCTGCATTGC

Product: heat shock protein ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MSESQSELDKYGTNLTKTAATNKLDPVIGRDAEIRRVCQILSRRTKNNPVLIGSAGVGKTAIVEGLAQRIVAGDVPESLR
NKEIVSLDLSALLAGASYRGEFEKRVKNLLEEIQKSKVIIFIDEVHTLMSAGAAEGAIAAGNMLKPLLARGELRLIGATT
LDEYREHLEKDPALERRFQQVFVGEPNLEDCIAIMRGLKERYEAHHKVSISDTALVTAVELSSRYITGRQLPDKAIDLLD
EAASSLRMEIDSSPVELDQLRREVDRLRLEELALKSENDPTSEQRLRSITNQLSGKQEMLNTLQASWNSERARLNQIGAL
KEQIDIAKQSADIAQREGRLEDASRLLYATIPQLQKNLAEKLEEQDSAPLVSDQVMPEDIASVVEGWTGIPVKKLMQKDA
KQLLHLEEDLSKSVIAQKVAIGVIADAVRRSRAGLSDPNRPSGTFLFLGPTGVGKTQLVKALASLLYDGEIVRIDMSEYS
EKFSISRLIGAPPGYIGHESAGQLTESVRRRPYSVVLFDEAEKAHPEVFDILLQVLDEGRLTDSHGRTVDFRNTIIVLTS
NIGSRYLSDISLEATTAHEYVNDEVRRTFRPEFLNRLDEIVIFEPLSQSDICQIVDLNIESLNKRIKDRRIVVTVSEDLR
RWLSKSGYDVIYGARPLRRLIQREIEDRLAKLIIEGLIHDGQTASFDLSGGAVNAQVCS

Sequences:

>Translated_699_residues
MSESQSELDKYGTNLTKTAATNKLDPVIGRDAEIRRVCQILSRRTKNNPVLIGSAGVGKTAIVEGLAQRIVAGDVPESLR
NKEIVSLDLSALLAGASYRGEFEKRVKNLLEEIQKSKVIIFIDEVHTLMSAGAAEGAIAAGNMLKPLLARGELRLIGATT
LDEYREHLEKDPALERRFQQVFVGEPNLEDCIAIMRGLKERYEAHHKVSISDTALVTAVELSSRYITGRQLPDKAIDLLD
EAASSLRMEIDSSPVELDQLRREVDRLRLEELALKSENDPTSEQRLRSITNQLSGKQEMLNTLQASWNSERARLNQIGAL
KEQIDIAKQSADIAQREGRLEDASRLLYATIPQLQKNLAEKLEEQDSAPLVSDQVMPEDIASVVEGWTGIPVKKLMQKDA
KQLLHLEEDLSKSVIAQKVAIGVIADAVRRSRAGLSDPNRPSGTFLFLGPTGVGKTQLVKALASLLYDGEIVRIDMSEYS
EKFSISRLIGAPPGYIGHESAGQLTESVRRRPYSVVLFDEAEKAHPEVFDILLQVLDEGRLTDSHGRTVDFRNTIIVLTS
NIGSRYLSDISLEATTAHEYVNDEVRRTFRPEFLNRLDEIVIFEPLSQSDICQIVDLNIESLNKRIKDRRIVVTVSEDLR
RWLSKSGYDVIYGARPLRRLIQREIEDRLAKLIIEGLIHDGQTASFDLSGGAVNAQVCS
>Mature_698_residues
SESQSELDKYGTNLTKTAATNKLDPVIGRDAEIRRVCQILSRRTKNNPVLIGSAGVGKTAIVEGLAQRIVAGDVPESLRN
KEIVSLDLSALLAGASYRGEFEKRVKNLLEEIQKSKVIIFIDEVHTLMSAGAAEGAIAAGNMLKPLLARGELRLIGATTL
DEYREHLEKDPALERRFQQVFVGEPNLEDCIAIMRGLKERYEAHHKVSISDTALVTAVELSSRYITGRQLPDKAIDLLDE
AASSLRMEIDSSPVELDQLRREVDRLRLEELALKSENDPTSEQRLRSITNQLSGKQEMLNTLQASWNSERARLNQIGALK
EQIDIAKQSADIAQREGRLEDASRLLYATIPQLQKNLAEKLEEQDSAPLVSDQVMPEDIASVVEGWTGIPVKKLMQKDAK
QLLHLEEDLSKSVIAQKVAIGVIADAVRRSRAGLSDPNRPSGTFLFLGPTGVGKTQLVKALASLLYDGEIVRIDMSEYSE
KFSISRLIGAPPGYIGHESAGQLTESVRRRPYSVVLFDEAEKAHPEVFDILLQVLDEGRLTDSHGRTVDFRNTIIVLTSN
IGSRYLSDISLEATTAHEYVNDEVRRTFRPEFLNRLDEIVIFEPLSQSDICQIVDLNIESLNKRIKDRRIVVTVSEDLRR
WLSKSGYDVIYGARPLRRLIQREIEDRLAKLIIEGLIHDGQTASFDLSGGAVNAQVCS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=311, Percent_Identity=34.7266881028939, Blast_Score=199, Evalue=1e-50,
Organism=Escherichia coli, GI1788943, Length=690, Percent_Identity=54.4927536231884, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=243, Percent_Identity=51.440329218107, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6320464, Length=689, Percent_Identity=52.1044992743106, Blast_Score=704, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=704, Percent_Identity=42.3295454545455, Blast_Score=556, Evalue=1e-159,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_TROWT (Q83FI1)

Other databases:

- EMBL:   AE014184
- RefSeq:   NP_787874.1
- ProteinModelPortal:   Q83FI1
- SMR:   Q83FI1
- STRING:   Q83FI1
- GeneID:   1482191
- GenomeReviews:   AE014184_GR
- KEGG:   twh:TWT746
- eggNOG:   COG0542
- HOGENOM:   HBG413133
- OMA:   HISNALN
- ProtClustDB:   CLSK229454
- BioCyc:   TWHI203267:TW746-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- PRINTS:   PR00300
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 77783; Mature: 77651

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESQSELDKYGTNLTKTAATNKLDPVIGRDAEIRRVCQILSRRTKNNPVLIGSAGVGKT
CCCCHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHH
AIVEGLAQRIVAGDVPESLRNKEIVSLDLSALLAGASYRGEFEKRVKNLLEEIQKSKVII
HHHHHHHHHHHCCCCCHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEE
FIDEVHTLMSAGAAEGAIAAGNMLKPLLARGELRLIGATTLDEYREHLEKDPALERRFQQ
EHHHHHHHHHCCCCCCCHHCCHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHH
VFVGEPNLEDCIAIMRGLKERYEAHHKVSISDTALVTAVELSSRYITGRQLPDKAIDLLD
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EAASSLRMEIDSSPVELDQLRREVDRLRLEELALKSENDPTSEQRLRSITNQLSGKQEML
HHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHH
NTLQASWNSERARLNQIGALKEQIDIAKQSADIAQREGRLEDASRLLYATIPQLQKNLAE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KLEEQDSAPLVSDQVMPEDIASVVEGWTGIPVKKLMQKDAKQLLHLEEDLSKSVIAQKVA
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVIADAVRRSRAGLSDPNRPSGTFLFLGPTGVGKTQLVKALASLLYDGEIVRIDMSEYS
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHH
EKFSISRLIGAPPGYIGHESAGQLTESVRRRPYSVVLFDEAEKAHPEVFDILLQVLDEGR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
LTDSHGRTVDFRNTIIVLTSNIGSRYLSDISLEATTAHEYVNDEVRRTFRPEFLNRLDEI
CCCCCCCEEEECCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHE
VIFEPLSQSDICQIVDLNIESLNKRIKDRRIVVTVSEDLRRWLSKSGYDVIYGARPLRRL
EEECCCCCCCCHHHEECCHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCEEEECCHHHHHH
IQREIEDRLAKLIIEGLIHDGQTASFDLSGGAVNAQVCS
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEECC
>Mature Secondary Structure 
SESQSELDKYGTNLTKTAATNKLDPVIGRDAEIRRVCQILSRRTKNNPVLIGSAGVGKT
CCCHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHH
AIVEGLAQRIVAGDVPESLRNKEIVSLDLSALLAGASYRGEFEKRVKNLLEEIQKSKVII
HHHHHHHHHHHCCCCCHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEE
FIDEVHTLMSAGAAEGAIAAGNMLKPLLARGELRLIGATTLDEYREHLEKDPALERRFQQ
EHHHHHHHHHCCCCCCCHHCCHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHH
VFVGEPNLEDCIAIMRGLKERYEAHHKVSISDTALVTAVELSSRYITGRQLPDKAIDLLD
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHH
EAASSLRMEIDSSPVELDQLRREVDRLRLEELALKSENDPTSEQRLRSITNQLSGKQEML
HHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHH
NTLQASWNSERARLNQIGALKEQIDIAKQSADIAQREGRLEDASRLLYATIPQLQKNLAE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
KLEEQDSAPLVSDQVMPEDIASVVEGWTGIPVKKLMQKDAKQLLHLEEDLSKSVIAQKVA
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVIADAVRRSRAGLSDPNRPSGTFLFLGPTGVGKTQLVKALASLLYDGEIVRIDMSEYS
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHH
EKFSISRLIGAPPGYIGHESAGQLTESVRRRPYSVVLFDEAEKAHPEVFDILLQVLDEGR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
LTDSHGRTVDFRNTIIVLTSNIGSRYLSDISLEATTAHEYVNDEVRRTFRPEFLNRLDEI
CCCCCCCEEEECCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHE
VIFEPLSQSDICQIVDLNIESLNKRIKDRRIVVTVSEDLRRWLSKSGYDVIYGARPLRRL
EEECCCCCCCCHHHEECCHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCEEEECCHHHHHH
IQREIEDRLAKLIIEGLIHDGQTASFDLSGGAVNAQVCS
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12902375