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The map label for this gene is ribB [H]

Identifier: 28572849

GI number: 28572849

Start: 801930

End: 802577

Strand: Direct

Name: ribB [H]

Synonym: TW708

Alternate gene names: 28572849

Gene position: 801930-802577 (Clockwise)

Preceding gene: 161501887

Following gene: 28572850

Centisome position: 86.61

GC content: 48.15

Gene sequence:

>648_bases
GTGCGACATATCCCCGATGCCCTGAATGCCCTTAGGGCCGGCAGGCCGGTTGTCGTCTTAGACGATAAAAATCGAGAAAA
CGAAGCGGATGTTATTATGGCCGCTCAGTTCGCAACACAAGAATGGGTTGCGTGGATTATCCGACATACAAGCGGGTTCC
TATGCGTACCTCTGTCAAATCAGAGGGCGGATAAGCTCCAATTACCTAGTATGGTAGCCGCAAATGAGGACCGTTTCAAA
ACAGCATACACAATAAGTGTTGATGCCGCAGACGCAGCAATAACAACCGGAATTTCTGCTGCAGACCGAAGCCTTACTAT
CAGGACGCTTGCCGCTCGGGACTCTTCTGCAAAACATCTTGTAAGGCCCGGGCATATACTACCTTTGAGAGCCAGAGACG
GTGGCCTTCTTGAGCGGCGCGGACACACCGAGGCGGCTATCGAATTGATGCGGCAAGCTCAACTTGAGCCAGTCGCTGTA
ATTGCAGAACTCGTATCAGATACGGGTGAGCTTTTAAGAGGCCGCGCGCTCGACGAGTTTATTCGTAAAAATAATCTAGT
TCTTGTAGAAATAAGTCAAATAATTGAATATATGAAAATGTCTGATCTAAAGGACATAAGTATGAATCTAAATCGCGACT
GCCTCTGA

Upstream 100 bases:

>100_bases
TTGTAAATCTTGAGACCGACGTCCTGGCAAGACATGTGCGCAGGTTCATGTCGCTTGTAAATTCATTTTCCTGAGGTATT
TTCGTAAGAATAGGCAGTTT

Downstream 100 bases:

>100_bases
GCGAGGGCTAAAAAGTATGATGGGGAATATGGATAAGGGTCTAGATTCGCAAAAACAAGAAACTGCTCGCAGCTGCAGAT
TTGAGGTTGAAACAAATCTT

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MRHIPDALNALRAGRPVVVLDDKNRENEADVIMAAQFATQEWVAWIIRHTSGFLCVPLSNQRADKLQLPSMVAANEDRFK
TAYTISVDAADAAITTGISAADRSLTIRTLAARDSSAKHLVRPGHILPLRARDGGLLERRGHTEAAIELMRQAQLEPVAV
IAELVSDTGELLRGRALDEFIRKNNLVLVEISQIIEYMKMSDLKDISMNLNRDCL

Sequences:

>Translated_215_residues
MRHIPDALNALRAGRPVVVLDDKNRENEADVIMAAQFATQEWVAWIIRHTSGFLCVPLSNQRADKLQLPSMVAANEDRFK
TAYTISVDAADAAITTGISAADRSLTIRTLAARDSSAKHLVRPGHILPLRARDGGLLERRGHTEAAIELMRQAQLEPVAV
IAELVSDTGELLRGRALDEFIRKNNLVLVEISQIIEYMKMSDLKDISMNLNRDCL
>Mature_215_residues
MRHIPDALNALRAGRPVVVLDDKNRENEADVIMAAQFATQEWVAWIIRHTSGFLCVPLSNQRADKLQLPSMVAANEDRFK
TAYTISVDAADAAITTGISAADRSLTIRTLAARDSSAKHLVRPGHILPLRARDGGLLERRGHTEAAIELMRQAQLEPVAV
IAELVSDTGELLRGRALDEFIRKNNLVLVEISQIIEYMKMSDLKDISMNLNRDCL

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=43.7810945273632, Blast_Score=174, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320695, Length=204, Percent_Identity=45.0980392156863, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 23848; Mature: 23848

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHIPDALNALRAGRPVVVLDDKNRENEADVIMAAQFATQEWVAWIIRHTSGFLCVPLSN
CCCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEECCC
QRADKLQLPSMVAANEDRFKTAYTISVDAADAAITTGISAADRSLTIRTLAARDSSAKHL
CCCCHHCCCHHHHCCCCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEEECCCCCCCCC
VRPGHILPLRARDGGLLERRGHTEAAIELMRQAQLEPVAVIAELVSDTGELLRGRALDEF
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IRKNNLVLVEISQIIEYMKMSDLKDISMNLNRDCL
HHHCCEEEEEHHHHHHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure
MRHIPDALNALRAGRPVVVLDDKNRENEADVIMAAQFATQEWVAWIIRHTSGFLCVPLSN
CCCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEEEEECCC
QRADKLQLPSMVAANEDRFKTAYTISVDAADAAITTGISAADRSLTIRTLAARDSSAKHL
CCCCHHCCCHHHHCCCCCCEEEEEEEECCCCHHHHCCCCCCCCEEEEEEEECCCCCCCCC
VRPGHILPLRARDGGLLERRGHTEAAIELMRQAQLEPVAVIAELVSDTGELLRGRALDEF
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IRKNNLVLVEISQIIEYMKMSDLKDISMNLNRDCL
HHHCCEEEEEHHHHHHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA