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The map label for this gene is gcvT [H]

Identifier: 28572801

GI number: 28572801

Start: 751396

End: 752466

Strand: Reverse

Name: gcvT [H]

Synonym: TW659

Alternate gene names: 28572801

Gene position: 752466-751396 (Counterclockwise)

Preceding gene: 28572802

Following gene: 28572800

Centisome position: 81.27

GC content: 46.78

Gene sequence:

>1071_bases
ATGGCCATGACTAGGGTATCTCCGCTTGATAACGAGCATAAAGCGCTTGGCGCGATTTTCACCTGTTTCGCGGGCTATAA
GTTGCCAGTGCGGTACAAGTCTGATATTGCGGAACACACTGCTGTTCGCCAGGGTTGTGGAATATTTGATCTGTCACATA
TGGCAGAAATATTTGTTTCAGGTGTGAACGCAGGCCTGGAATTGGATATTGCTCTCACAGGGCACTTCTCTGATATGACT
TGTGGGCGCGCAAAATACACGCTTATTTTGAATGAACAGGGCGGCATAGAGGATGATCTGATTGTCTACCGAATTGATGA
TAAAAATTACATGGTTGTTGCAAATGCGATAAATCGCAAGAAGGTGTTTTCTCTCTTGCGAGATAGAGTGCATACATCAG
ATATCAAGGATGAGACTGATTCGATATCTCTTGTAGCTGTGCAGGGCCCGGAATCTGAGAGTCTAATAAGAGATCTATTT
CATGAATCCGGTAATTTACGCTATTTCTCCCACCGGTGTTATCCACACGGCGGAACGGATCGTTCCGAGAAATTAAGTTG
TTCAATTGTTGCCCGAACGGGATACACAGGGGAAGATGGCTTTGAAATCTTTACCCCAAACGATAGCGTGGTATCTATTT
GGCGAGCCCTCATAGAGCGAGGTGCGACTCCTTGCGGACTCGCTGCTCGCAATACCCTTCGAATAGAGGCGGGTATGCCT
TTGTACGGCCACGAACTTCGGGCGGATTTAAATCCAGTGCAGGCAGGACTCGAGAGATTTATTTCGCCTCCGTGTATCGG
TTGTGACAGAATAAATCATTCAGGTGATTTTCCCTTACTCGTTGGCTTATCTATCGACGGGCGCCGTGCTGCCCGTGAGG
GATATACCATTTACAGCAGCTCTAATATCCCCGTTGGGACTGTTACAAGTGGATGTTTCTCTCCAACCTTGGGCTGCTCA
ATCGCTATGGCTTATGTTAAAGCTAAAGCGCGCAGTGCGCAACTTTATATTGATATTAGAGGCTCCATGACGCCATGTAA
AGTTGTTCCCATGCCGTTTTATAGACGTTGA

Upstream 100 bases:

>100_bases
GGTGCAGTCCAATCTCACGATACTCTTCAGTCTCACACTTGTACCCCAGTCTATCCGTATCTTTCAGGAGTTCAACAGAC
ATGTAGTACCCTAGTCAAGG

Downstream 100 bases:

>100_bases
AGGTAAAGTACTAGTTCGAGTGTAATTTAGAGACGGCGGGATTTTTGGAGGCAGAGTGTTCGATGAAAGTGACCTGGTCT
ACAGCAAGGAACATGAGTGG

Product: aminomethyltransferase gcvT

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAMTRVSPLDNEHKALGAIFTCFAGYKLPVRYKSDIAEHTAVRQGCGIFDLSHMAEIFVSGVNAGLELDIALTGHFSDMT
CGRAKYTLILNEQGGIEDDLIVYRIDDKNYMVVANAINRKKVFSLLRDRVHTSDIKDETDSISLVAVQGPESESLIRDLF
HESGNLRYFSHRCYPHGGTDRSEKLSCSIVARTGYTGEDGFEIFTPNDSVVSIWRALIERGATPCGLAARNTLRIEAGMP
LYGHELRADLNPVQAGLERFISPPCIGCDRINHSGDFPLLVGLSIDGRRAAREGYTIYSSSNIPVGTVTSGCFSPTLGCS
IAMAYVKAKARSAQLYIDIRGSMTPCKVVPMPFYRR

Sequences:

>Translated_356_residues
MAMTRVSPLDNEHKALGAIFTCFAGYKLPVRYKSDIAEHTAVRQGCGIFDLSHMAEIFVSGVNAGLELDIALTGHFSDMT
CGRAKYTLILNEQGGIEDDLIVYRIDDKNYMVVANAINRKKVFSLLRDRVHTSDIKDETDSISLVAVQGPESESLIRDLF
HESGNLRYFSHRCYPHGGTDRSEKLSCSIVARTGYTGEDGFEIFTPNDSVVSIWRALIERGATPCGLAARNTLRIEAGMP
LYGHELRADLNPVQAGLERFISPPCIGCDRINHSGDFPLLVGLSIDGRRAAREGYTIYSSSNIPVGTVTSGCFSPTLGCS
IAMAYVKAKARSAQLYIDIRGSMTPCKVVPMPFYRR
>Mature_355_residues
AMTRVSPLDNEHKALGAIFTCFAGYKLPVRYKSDIAEHTAVRQGCGIFDLSHMAEIFVSGVNAGLELDIALTGHFSDMTC
GRAKYTLILNEQGGIEDDLIVYRIDDKNYMVVANAINRKKVFSLLRDRVHTSDIKDETDSISLVAVQGPESESLIRDLFH
ESGNLRYFSHRCYPHGGTDRSEKLSCSIVARTGYTGEDGFEIFTPNDSVVSIWRALIERGATPCGLAARNTLRIEAGMPL
YGHELRADLNPVQAGLERFISPPCIGCDRINHSGDFPLLVGLSIDGRRAAREGYTIYSSSNIPVGTVTSGCFSPTLGCSI
AMAYVKAKARSAQLYIDIRGSMTPCKVVPMPFYRR

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=381, Percent_Identity=30.9711286089239, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI257796258, Length=352, Percent_Identity=31.8181818181818, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI257796256, Length=313, Percent_Identity=30.0319488817891, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI257796254, Length=376, Percent_Identity=26.8617021276596, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI197927446, Length=255, Percent_Identity=24.7058823529412, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI21361378, Length=255, Percent_Identity=24.7058823529412, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI24797151, Length=339, Percent_Identity=23.8938053097345, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789272, Length=368, Percent_Identity=34.5108695652174, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17560118, Length=382, Percent_Identity=30.3664921465969, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32563613, Length=405, Percent_Identity=22.2222222222222, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320222, Length=376, Percent_Identity=30.8510638297872, Blast_Score=160, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129441, Length=376, Percent_Identity=32.4468085106383, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI20130091, Length=248, Percent_Identity=24.5967741935484, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571104, Length=293, Percent_Identity=23.2081911262799, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39134; Mature: 39003

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTRVSPLDNEHKALGAIFTCFAGYKLPVRYKSDIAEHTAVRQGCGIFDLSHMAEIFVS
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCEECHHHHHHHHHH
GVNAGLELDIALTGHFSDMTCGRAKYTLILNEQGGIEDDLIVYRIDDKNYMVVANAINRK
CCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEEECCCHH
KVFSLLRDRVHTSDIKDETDSISLVAVQGPESESLIRDLFHESGNLRYFSHRCYPHGGTD
HHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCC
RSEKLSCSIVARTGYTGEDGFEIFTPNDSVVSIWRALIERGATPCGLAARNTLRIEAGMP
CCCEEEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
LYGHELRADLNPVQAGLERFISPPCIGCDRINHSGDFPLLVGLSIDGRRAAREGYTIYSS
CCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCEEEEEC
SNIPVGTVTSGCFSPTLGCSIAMAYVKAKARSAQLYIDIRGSMTPCKVVPMPFYRR
CCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCC
>Mature Secondary Structure 
AMTRVSPLDNEHKALGAIFTCFAGYKLPVRYKSDIAEHTAVRQGCGIFDLSHMAEIFVS
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCEECHHHHHHHHHH
GVNAGLELDIALTGHFSDMTCGRAKYTLILNEQGGIEDDLIVYRIDDKNYMVVANAINRK
CCCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEEECCCHH
KVFSLLRDRVHTSDIKDETDSISLVAVQGPESESLIRDLFHESGNLRYFSHRCYPHGGTD
HHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCC
RSEKLSCSIVARTGYTGEDGFEIFTPNDSVVSIWRALIERGATPCGLAARNTLRIEAGMP
CCCEEEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
LYGHELRADLNPVQAGLERFISPPCIGCDRINHSGDFPLLVGLSIDGRRAAREGYTIYSS
CCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHCCCEEEEEC
SNIPVGTVTSGCFSPTLGCSIAMAYVKAKARSAQLYIDIRGSMTPCKVVPMPFYRR
CCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA