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The map label for this gene is gcvH

Identifier: 28572800

GI number: 28572800

Start: 750975

End: 751340

Strand: Reverse

Name: gcvH

Synonym: TW658

Alternate gene names: 28572800

Gene position: 751340-750975 (Counterclockwise)

Preceding gene: 28572801

Following gene: 28572799

Centisome position: 81.14

GC content: 42.62

Gene sequence:

>366_bases
GTGTTCGATGAAAGTGACCTGGTCTACAGCAAGGAACATGAGTGGGTTTTTATCGATGATGATATTGCCTGGGTTGGTAT
TACAAAATATGCCGTGAAAAAACTTGGCGATATTGTCTACATTGACCTTCCATCGCAAGATGCTCTTATAGTTCAGGGTG
AGTGTGTTGGGGAAATTGAGTCAACTAAATCCGTCAGCGAAATATACTCGCCTGTTTCCGGAAGGGTTATAGCAGTTAAT
GAGGATGTTATTTCGTCCCCCGGATTATTGAACTCAGATTCCTCTGTGTGGCTTTTCAAAGCTGAGTGTGAAAATATTCC
AGAATTGATGGACTGGGGCCAGTACAGCGAATACACGAAAGAATAG

Upstream 100 bases:

>100_bases
ATGACGCCATGTAAAGTTGTTCCCATGCCGTTTTATAGACGTTGAAGGTAAAGTACTAGTTCGAGTGTAATTTAGAGACG
GCGGGATTTTTGGAGGCAGA

Downstream 100 bases:

>100_bases
CCCCCTCCAGTCTCGGCTGGGCACTTGAAGATGTCAGATTTGCTGGATGCTTTGCACCTCTTTCTGATACATCGTTAGCC
CAACCCCTTCAACCAGTAAC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 121

Protein sequence:

>121_residues
MFDESDLVYSKEHEWVFIDDDIAWVGITKYAVKKLGDIVYIDLPSQDALIVQGECVGEIESTKSVSEIYSPVSGRVIAVN
EDVISSPGLLNSDSSVWLFKAECENIPELMDWGQYSEYTKE

Sequences:

>Translated_121_residues
MFDESDLVYSKEHEWVFIDDDIAWVGITKYAVKKLGDIVYIDLPSQDALIVQGECVGEIESTKSVSEIYSPVSGRVIAVN
EDVISSPGLLNSDSSVWLFKAECENIPELMDWGQYSEYTKE
>Mature_121_residues
MFDESDLVYSKEHEWVFIDDDIAWVGITKYAVKKLGDIVYIDLPSQDALIVQGECVGEIESTKSVSEIYSPVSGRVIAVN
EDVISSPGLLNSDSSVWLFKAECENIPELMDWGQYSEYTKE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=37.6068376068376, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=37.6068376068376, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789271, Length=108, Percent_Identity=42.5925925925926, Blast_Score=85, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551294, Length=125, Percent_Identity=35.2, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=122, Percent_Identity=36.0655737704918, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319272, Length=116, Percent_Identity=42.2413793103448, Blast_Score=89, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=41.3793103448276, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_TROW8 (P64216)

Other databases:

- EMBL:   BX251412
- RefSeq:   NP_789580.1
- ProteinModelPortal:   P64216
- SMR:   P64216
- STRING:   P64216
- GeneID:   1064859
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW658
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   VIGITAY
- ProtClustDB:   CLSK230181
- BioCyc:   TWHI218496:TW0625-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13618; Mature: 13618

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDESDLVYSKEHEWVFIDDDIAWVGITKYAVKKLGDIVYIDLPSQDALIVQGECVGEIE
CCCCCCCEEECCCCEEEECCCEEEEEHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCC
STKSVSEIYSPVSGRVIAVNEDVISSPGLLNSDSSVWLFKAECENIPELMDWGQYSEYTK
CHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCC
E
C
>Mature Secondary Structure
MFDESDLVYSKEHEWVFIDDDIAWVGITKYAVKKLGDIVYIDLPSQDALIVQGECVGEIE
CCCCCCCEEECCCCEEEECCCEEEEEHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCC
STKSVSEIYSPVSGRVIAVNEDVISSPGLLNSDSSVWLFKAECENIPELMDWGQYSEYTK
CHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174